MCQOPTIONS
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MCQOPTIONS
This section includes 163 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Mutant strains of Saccharomyces cervevisiae in which endogenous DNA are deleted are called as _________ |
| A. | rho0 |
| B. | synthetic rho |
| C. | rho+ |
| D. | rho- |
| Answer» B. synthetic rho | |
| 2. |
Schizosaccharomyces pombe is yeast and it reproduces by __________ |
| A. | budding |
| B. | fissing |
| C. | either budding or fissing |
| D. | a method of reproduction other than budding or fissing |
| Answer» C. either budding or fissing | |
| 3. |
Protoplast transformation is less time consuming than lithium acetate for transformation. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» C. May be True or False | |
| 4. |
Splicing of the transcripts is necessary for efficient expression. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 5. |
In ___________ cells, virus replication doesn’t take place and viral DNA ___________ |
| A. | non-permissive, is also not expressed |
| B. | permissive, is also not expressed |
| C. | non-permissive, can be expressed |
| D. | non-permissive, is always expressed |
| Answer» D. non-permissive, is always expressed | |
| 6. |
SV40 is a virus and it produces how many transcripts? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 7. |
DEAE-dextran is used for introduction of DNA. It is a modified _____ and is ______ |
| A. | polysaccharide, negatively |
| B. | polysaccharide, positively |
| C. | monosaccharide, positively |
| D. | monosaccharide, negatively |
| Answer» C. monosaccharide, positively | |
| 8. |
Many mammalian cells contain Thymidine Kinase, the mammalian enzyme uses the analogue _____ than does the viral enzyme. |
| A. | more efficiently |
| B. | less efficiently |
| C. | with same efficiency |
| D. | either with same or more efficiency |
| Answer» C. with same efficiency | |
| 9. |
Only the _____ part of Drosophila is transgenic and the rest is not. This is known as ______ |
| A. | germline cell, mosaic |
| B. | nurse cells, mosaic |
| C. | germline cells, hybrid |
| D. | nurse cells, hybrid |
| Answer» B. nurse cells, mosaic | |
| 10. |
Vaccinia virus is a commonly used virus and it is a member of pox virus family. It has a large genome of _____________ and can accept at least ____________ of foreign DNA. |
| A. | 200kb, 25kb |
| B. | 1000kb, 200kb |
| C. | 500kb, 50kb |
| D. | 800kb, 100kb |
| Answer» B. 1000kb, 200kb | |
| 11. |
Which of the following compounds control ripening in tomatoes? |
| A. | Auxin |
| B. | Cytokinin |
| C. | Ethylene |
| D. | Jasmonic acid |
| Answer» D. Jasmonic acid | |
| 12. |
Tetracycline binding repressor protein when fused to transcription activator protein from herpes simple virus, then it is called as transactivator protein (tTA). |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 13. |
Resistance to bleomycin (zeocin) is used as a selectable marker for mammalian cultured cells and its function is ____________ |
| A. | DNA damaging agent |
| B. | DNA synthesis promoter |
| C. | Inhibiting RNA synthesis |
| D. | Activating RNA synthesis |
| Answer» B. DNA synthesis promoter | |
| 14. |
Centromere is the most important component of the human artificial chromosome. It is constructed from tandemly repeated copies of 171 bp of ______ DNA sequence. |
| A. | alpha satellite |
| B. | beta satellite |
| C. | satellite 2 |
| D. | satellite 1 |
| Answer» B. beta satellite | |
| 15. |
DNA can be injected into Caenorhabditis elegans by biolistic transformation. The injected DNA forms arrays of extrochromosomal copies which are stable in nature. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» C. May be True or False | |
| 16. |
Resistance to methotrexate, which inhibits the enzyme dihydrofolate reductase (DHFR) is used as a selectable marker. This enzyme is involved in the synthesis of _____ carbon units and is required for _____ biosynthesis. |
| A. | two, nucleoside |
| B. | two, nucleotide |
| C. | one, nucleotide |
| D. | one, nucleoside |
| Answer» E. | |
| 17. |
Integration of DNA into the Chlamydomonas genome is via homologous recombination. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» C. May be True or False | |
| 18. |
Mitochondrial genome encodes tRNAs ___________ and polypeptides involved in _______ |
| A. | mRNAs, oxidative phosphorylation |
| B. | rRNAs, oxidative phosphorylation |
| C. | rRNAs, reductive phosphorylation |
| D. | mRNAs, reductive phosphorylation |
| Answer» C. rRNAs, reductive phosphorylation | |
| 19. |
LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector. |
| A. | eukaryotic plasmid |
| B. | prokaryotic plasmid |
| C. | either eukaryotic or prokaryotic plasmid |
| D. | yeast plasmid |
| Answer» C. either eukaryotic or prokaryotic plasmid | |
| 20. |
Glucorticoid-responsive element is ______ promoter from ______ of mouse mammory tumour virus (MMTV). |
| A. | inducible, short terminal repeat region |
| B. | inducible, long terminal repeat region |
| C. | constitutive, short terminal repeat region |
| D. | constitutive, long terminal repeat region |
| Answer» C. constitutive, short terminal repeat region | |
| 21. |
Barstar is _________ |
| A. | RNAse |
| B. | RNAse inhibitor |
| C. | DNAse |
| D. | DNAse inhibitor |
| Answer» C. DNAse | |
| 22. |
There are a group of promoters from the genes for metallothioneins. These are a group of proteins rich in ______ residues. |
| A. | cysteine |
| B. | methionine |
| C. | either cysteine or methionine |
| D. | cysteine and methionine both |
| Answer» B. methionine | |
| 23. |
If YAC DNA is digested with BamHI and ECoRI, how many fragments are generated? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» D. 4 | |
| 24. |
For the generation of recombinant baculovirus, recombinants can be selected by ____________ |
| A. | blue-white screening |
| B. | antibiotic resistance |
| C. | either antibiotic resistance or blue white screening |
| D. | radioactivity |
| Answer» B. antibiotic resistance | |
| 25. |
Constitutive promoters are majorly obtained from ________ |
| A. | fungi |
| B. | bacteria |
| C. | mammalian cells |
| D. | viral cells |
| Answer» E. | |
| 26. |
Autographa californica nuclear polyhedrois virus (AcNPV) is commonly used bacilovirus for infecting cultured cells. The virus is ________ and _______ |
| A. | single stranded, linear |
| B. | double stranded, linear |
| C. | double stranded, circular |
| D. | single stranded, circular |
| Answer» D. single stranded, circular | |
| 27. |
Secretion of overexpressed proteins by fusion to a secretion signal can be useful because it helps in bringing about correct _______ and _____ protein folding. |
| A. | glycosylation, enhance |
| B. | glycosylation, not affect |
| C. | phosphorylation, enhance |
| D. | phosphorylation, not affect |
| Answer» B. glycosylation, not affect | |
| 28. |
Which of the following are advantageous to be used for expression of eukaryotic cells? |
| A. | Prokaryotic systems |
| B. | Yeast cells |
| C. | Fungi cells |
| D. | Algae cells |
| Answer» C. Fungi cells | |
| 29. |
The leu1+ marker encodes which enzyme? |
| A. | orotidine-5’-phosphate decarboxylase |
| B. | beta isopropylmalate dehydrogenase |
| C. | orotidine-5’-phosphate decarboxylase or beta isopropylmalate dehydrogenase |
| D. | orotidine-5’-phosphate decarboxylase & beta isopropylmalate dehydrogenase |
| Answer» C. orotidine-5’-phosphate decarboxylase or beta isopropylmalate dehydrogenase | |
| 30. |
Wild-type Drosophila flies are _____ to ethanol supplied in food. |
| A. | resistant |
| B. | non-resistant |
| C. | resistant at low concentration and non-resistant at higher concentration |
| D. | resistant at higher concentrations and non-resistant at lower concentration |
| Answer» B. non-resistant | |
| 31. |
Presence of wild-type DNA onto the HGPRT– in the presence of _______ led to DNA uptake and stable transformation. |
| A. | lithium acetate |
| B. | calcium phosphate |
| C. | sodium chloride |
| D. | aluminum sulphate |
| Answer» C. sodium chloride | |
| 32. |
If transfer of DNA from Agrobacterium to plants is done via incubation of explanted material and the vector containing DNA of interest and then selection is done via selectable marker then this method is called as _________ |
| A. | transformation |
| B. | co-cultivation |
| C. | co-transformation |
| D. | floral dipping |
| Answer» C. co-transformation | |
| 33. |
Allotopic gene expression is the case when the presence of a normal gene in an organelle is not a problem in expression. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 34. |
Transfer of T DNA depends on a set of genes called as ______ if they are present on ________ |
| A. | vir, chromosome |
| B. | chv, tumour inducing plasmid |
| C. | chv, chromosome |
| D. | vir, whether they are present on tumour inducing plasmid or chromosome |
| Answer» D. vir, whether they are present on tumour inducing plasmid or chromosome | |
| 35. |
______ integration systems are used for the transfer of DNA in Drosophila and it is composed of ______ |
| A. | Artificial, P elements |
| B. | Artificial, S elements |
| C. | Natural, P elements |
| D. | Natural, S elements |
| Answer» D. Natural, S elements | |
| 36. |
Expression of tTA gene is ________ by doxycycline which is _______ analogue. |
| A. | activated, tetracycline |
| B. | inactivated, chloramphenicol |
| C. | activated, chloramphenicol |
| D. | inactivated, tetracycline |
| Answer» E. | |
| 37. |
Chlamyodomonas genome is _______ rich. |
| A. | AT |
| B. | GC |
| C. | Only A |
| D. | Only C |
| Answer» C. Only A | |
| 38. |
Choose the incorrect statement in respect to MACs. |
| A. | They are mammalian or human artificial chromosomes |
| B. | Large stretches of DNA (in excess of 100 kb) can be inserted |
| C. | A selectable marker is required |
| D. | It can be maintained for long term |
| Answer» D. It can be maintained for long term | |
| 39. |
The gene of interest is inserted into transfer vector and then further into viral genome leading to the formation of modified virus. How can plaques from modified virus differentiated from plaques from wild-type virus? |
| A. | Chemically |
| B. | Visually |
| C. | On the basis of magnetic properties |
| D. | On the basis of longeitivity of plaques |
| Answer» C. On the basis of magnetic properties | |
| 40. |
For transformation of the chloroplast of higher plants, a vector is used which ______ in the chloroplast. |
| A. | doesn’t replicates |
| B. | replicates |
| C. | may or may not replicate |
| D. | replicates under certain specified conditions |
| Answer» B. replicates | |
| 41. |
The amount of expression of DNA inserted into a vector _______ on promoter and ______ on chromosomal location. |
| A. | depends only on, not depends |
| B. | depends, depends also |
| C. | doesn’t depends, but depends |
| D. | neither depends, nor depends |
| Answer» C. doesn’t depends, but depends | |
| 42. |
Bacterial aadA gene is responsible for conferring resistance to _________ |
| A. | spectinomycin |
| B. | streptomycin |
| C. | ampicillin |
| D. | spectomycin and streptomycin |
| Answer» E. | |
| 43. |
Promoters such as GAL1 can be used to direct synthesis of either unmodified or fusion proteins. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 44. |
Where do P elements integrate into the genome? |
| A. | At specifically defined sites |
| B. | Randomly |
| C. | Only at the ends of the genome |
| D. | Integration depends on reaction conditions |
| Answer» C. Only at the ends of the genome | |
| 45. |
The recombinant virus can be propagated in E.coli and this is referred to as bacmid. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 46. |
GAL1 is a controllable promoter and is obtained from genes for galactose metabolism. It is known for encoding ______ |
| A. | UDP-galactose-4-empirase |
| B. | Galactokinase |
| C. | Both UDP-galactose-4-empirase and galactokinase |
| D. | Glucokinase |
| Answer» C. Both UDP-galactose-4-empirase and galactokinase | |
| 47. |
Cis-acting sites should be present in the vector for ________ |
| A. | replication |
| B. | selecting recombinants by acting as a marker |
| C. | transposition |
| D. | providing high copy number |
| Answer» D. providing high copy number | |
| 48. |
ES cells are used in order to ensure that insertion is done at the required chromosomal location and it is called as ____________ |
| A. | gene targeting |
| B. | knocking out |
| C. | knocking in |
| D. | gene disruption |
| Answer» B. knocking out | |
| 49. |
Embryonic stem cells are also used for generation of transgenic organisms. They are obtained from _______ of a developing ________ |
| A. | trophoectoderm, gastrula |
| B. | trophoectoderm, blastula |
| C. | inner cell mass, blastula |
| D. | inner cell mass, gastrula |
| Answer» D. inner cell mass, gastrula | |
| 50. |
Promoters can be divided basically into how many types? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |