AT_TIMES,_A_SPECIFIC_FRAGMENT_OF_THE_MOLECULES_OF_DNA_WHICH_ARE_ANALYZED_NEEDS_TO_BE_SEPARATED._ONE_OF_THE_METHODS_IS_DIGESTING_THE_GELS_SIMPLY._WHICH_OF_THE_FOLLOWING_STATEMENT_IS_NOT_CORRECT_IN_WITH_RESPECT_TO_IT??$

The gelling temperature has an important role to play

For higher gelling temperatures, digestion of DNA is done via addition of agarase or some chaotropic agents

For lower gelling temperatures, simply slicing out of target DNA is done which is followed by melting

The DNA can be extracted via addition of sodium or any positively charged group

If the amount of agarose added is more, the molecular under analysis should have following characteristsic?

size has no relation with the amount of agarose

the amount of molecules under analysis matters

Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels?

TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking

They are obtained via polymerization between acrylamide and bis-acrylamide

The components added for initiating polymerization are ammonium persulphate and TEMED

It is casted in horizontal and flat trays

TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking

Gel matrices are generally of two types Agarose and Polyacrylamide. Which of the statements is correct with respect to agarose gels?

Agarose is a polysaccharide obtained from fungus

Agarose is a polysaccharide which is obtained from red algae

Agarose is a polysaccharide obtained from fungus

It is composed of glucose residues

It is obtained via dissolving in it water by boiling in water and then cooling it

How is the size of molecules under analysis measured?

There is no exact criterion for doing so

By measuring the distance moved through a ruler

By measuring the amount of visualising dye used

By running a standard molecule, whose size is known in parallel

There is no exact criterion for doing so

Which one of the following will travel fastest through the gel if the amount of DNA present is same in all?

Supercoiled and circular will move at same speed and faster than nicked

The charge present on the DNA backbone is negative. The force required to accelerate the molecules towards anode is directly proportional to number of:

both phosphate group and sugar molecules

Gel electrophoresis separates nucleic acid molecules based on:

nature of the molecules i.e. whether DNA or RNA

chemical properties of the nucleic acids

10 + Mcqs in Gel Electrophoresis Oligonucleotide Microarrays in Genetic Engineering Page 1 McqOptions

1.	AT_TIMES,_A_SPECIFIC_FRAGMENT_OF_THE_MOLECULES_OF_DNA_WHICH_ARE_ANALYZED_NEEDS_TO_BE_SEPARATED._ONE_OF_THE_METHODS_IS_DIGESTING_THE_GELS_SIMPLY._WHICH_OF_THE_FOLLOWING_STATEMENT_IS_NOT_CORRECT_IN_WITH_RESPECT_TO_IT??$
A.	The gelling temperature has an important role to play
B.	For higher gelling temperatures, digestion of DNA is done via addition of agarase or some chaotropic agents
C.	For lower gelling temperatures, simply slicing out of target DNA is done which is followed by melting
D.	The DNA can be extracted via addition of sodium or any positively charged group
Answer» E.

Discussion

2.	In the case of electrophoresis of single stranded DNA or RNA, which type of gels are used?
A.	Renaturing
B.	Denaturing
C.	The routine agarose gel
D.	The routine polyacrylamide gel
Answer» C. The routine agarose gel

Discussion

3.	If the amount of agarose added is more, the molecular under analysis should have following characteristsic?
A.	small size
B.	large size
C.	size has no relation with the amount of agarose
D.	the amount of molecules under analysis matters
Answer» B. large size

Discussion

4.	Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels?
A.	They are obtained via polymerization between acrylamide and bis-acrylamide
B.	The components added for initiating polymerization are ammonium persulphate and TEMED
C.	It is casted in horizontal and flat trays
D.	TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking
Answer» D. TEMED catalyses the formation of free radicals from persulphate ions which leads to initiation of cross-linking

Discussion

5.	Gel matrices are generally of two types Agarose and Polyacrylamide. Which of the statements is correct with respect to agarose gels?
A.	Agarose is a polysaccharide which is obtained from red algae
B.	Agarose is a polysaccharide obtained from fungus
C.	It is composed of glucose residues
D.	It is obtained via dissolving in it water by boiling in water and then cooling it
Answer» B. Agarose is a polysaccharide obtained from fungus

Discussion

6.	How is the size of molecules under analysis measured?
A.	By measuring the distance moved through a ruler
B.	By measuring the amount of visualising dye used
C.	By running a standard molecule, whose size is known in parallel
D.	There is no exact criterion for doing so
Answer» D. There is no exact criterion for doing so

Discussion

7.	Which one of the following will travel fastest through the gel if the amount of DNA present is same in all?
A.	Circular
B.	Supercoiled
C.	Nicked
D.	Supercoiled and circular will move at same speed and faster than nicked
Answer» C. Nicked

Discussion

8.	Force is defined as mass per unit acceleration. As the number of phosphate molecules increase, the charge also increases which increases the force required. The acceleration is dependent of the size of the molecules. The given statement is true or false?
A.	True
B.	False
Answer» C.

Discussion

9.	The charge present on the DNA backbone is negative. The force required to accelerate the molecules towards anode is directly proportional to number of:
A.	sugar molecules
B.	nitrogenous bases
C.	phosphate groups
D.	both phosphate group and sugar molecules
Answer» D. both phosphate group and sugar molecules

Discussion

10.	Gel electrophoresis separates nucleic acid molecules based on:
A.	charge on molecules
B.	size of the molecules
C.	nature of the molecules i.e. whether DNA or RNA
D.	chemical properties of the nucleic acids
Answer» C. nature of the molecules i.e. whether DNA or RNA

Discussion

Explore topic-wise MCQs in Genetic Engineering.

AT_TIMES,_A_SPECIFIC_FRAGMENT_OF_THE_MOLECULES_OF_DNA_WHICH_ARE_ANALYZED_NEEDS_TO_BE_SEPARATED._ONE_OF_THE_METHODS_IS_DIGESTING_THE_GELS_SIMPLY._WHICH_OF_THE_FOLLOWING_STATEMENT_IS_NOT_CORRECT_IN_WITH_RESPECT_TO_IT??$

In the case of electrophoresis of single stranded DNA or RNA, which type of gels are used?

If the amount of agarose added is more, the molecular under analysis should have following characteristsic?

Polyacrylamide gels are the other types of gels which are commonly used. Which of the following statement is not correct with respect to these types of gels?

Gel matrices are generally of two types Agarose and Polyacrylamide. Which of the statements is correct with respect to agarose gels?

How is the size of molecules under analysis measured?

Which one of the following will travel fastest through the gel if the amount of DNA present is same in all?

Force is defined as mass per unit acceleration. As the number of phosphate molecules increase, the charge also increases which increases the force required. The acceleration is dependent of the size of the molecules. The given statement is true or false?

The charge present on the DNA backbone is negative. The force required to accelerate the molecules towards anode is directly proportional to number of:

Gel electrophoresis separates nucleic acid molecules based on: