Sequences that can function as origins of replication are called as ___________

autonomously replicating sequences

Sequences that can function as origins of replication are called as:

autonomously replicating sequences

Luciferase genes are also used at times for detection. Choose the correct statement for them.

Enzymes such as beta-galactosidase requires substrate X-gluc to produce light

They are obtained from fire flies only

The detection requires provision of substrate which produces light

Enzymes such as beta-galactosidase requires substrate X-gluc to produce light

Lucifearse genes are preferred over fluorescent proteins

GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.

The absorbed blue light produces green light which can be detected further

It stands for Green Fluorescent Protein

It is obtained from a bio-luminescent jellyfish and produces a protein aequorin which emits blue light

The blue light is produced because of binding of sodium ions

The absorbed blue light produces green light which can be detected further

Promoter-probe vectors are used often. Choose the correct statement for these vectors.

It contains a reporter gene which contains its promoter along

They are used for identifying sequences that can function as promoter in vivo

It contains a reporter gene which contains its promoter along

The reporter gene is having a cloning site

After insertion of DNA into the cloning site, selection of plasmids is carried out by blue white screening

16 + Mcqs in Reporter Genes in Genetic Engineering Page 1 McqOptions

1.	Protein-protein interactions such as in electron transport lead to activation of the reporter gene.
A.	True
B.	False
Answer» C.

Discussion

2.	_____ should enter the cell in the case of activation of the reporter gene for two hybrid system.
A.	Bait protein
B.	Prey protein
C.	Both bait and prey protein
D.	Either one of them
Answer» D. Either one of them

Discussion

3.	For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein.
A.	prey
B.	bait
C.	binding
D.	activation
Answer» C. binding

Discussion

4.	Sequences that can function as origins of replication are called as ___________
A.	partial replicating sequences
B.	self replicating sequences
C.	autonomously replicating sequences
D.	modified replicating sequences
Answer» D. modified replicating sequences

Discussion

5.	In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened.
A.	True
B.	False
Answer» B. False

Discussion

6.	______SHOULD_ENTER_THE_CELL_IN_THE_CASE_OF_ACTIVATION_OF_REPORTER_GENE_FOR_TWO_HYBRID_SYSTEM.?$
A.	Bait protein
B.	Prey protein
C.	Both bait and prey protein
D.	Either one of them
Answer» D. Either one of them

Discussion

7.	Protein-protein interactions such as in electron transport lead to activation of reporter gene. Is the given statement true or false?$
A.	True
B.	False
Answer» C.

Discussion

8.	The transcription domain is ________ if some of the bait and prey proteins are non-specific in nature?
A.	deactivated
B.	activated
C.	destroyed
D.	may be activated or not
Answer» E.

Discussion

9.	For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein?
A.	prey
B.	bait
C.	binding
D.	activation
Answer» C. binding

Discussion

10.	In two hybrid screening system, the activator binds through _______ domain to a sequence upstream of the gene under its control, and ________ domain stimulates transcription.
A.	DNA binding, activation
B.	Activation, DNA binding
C.	Activation, transcription
D.	DNA binding, transcription
Answer» B. Activation, DNA binding

Discussion

11.	Screening is often carried out for a sequence that interacts with a protein for which already a clone is present. It is carried out in which host?
A.	Bacterial host
B.	Fungal host
C.	Parasitic host
D.	Yeast host
Answer» E.

Discussion

12.	Sequences that can function as origins of replication are called as:
A.	partial replicating sequences
B.	self replicating sequences
C.	autonomously replicating sequences
D.	modified replicating sequences
Answer» D. modified replicating sequences

Discussion

13.	In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened. Is the given statement true or false?
A.	True
B.	False
Answer» B. False

Discussion

14.	Luciferase genes are also used at times for detection. Choose the correct statement for them.
A.	They are obtained from fire flies only
B.	The detection requires provision of substrate which produces light
C.	Enzymes such as beta-galactosidase requires substrate X-gluc to produce light
D.	Lucifearse genes are preferred over fluorescent proteins
Answer» C. Enzymes such as beta-galactosidase requires substrate X-gluc to produce light

Discussion

15.	GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.
A.	It stands for Green Fluorescent Protein
B.	It is obtained from a bio-luminescent jellyfish and produces a protein aequorin which emits blue light
C.	The blue light is produced because of binding of sodium ions
D.	The absorbed blue light produces green light which can be detected further
Answer» D. The absorbed blue light produces green light which can be detected further

Discussion

16.	Promoter-probe vectors are used often. Choose the correct statement for these vectors.
A.	They are used for identifying sequences that can function as promoter in vivo
B.	It contains a reporter gene which contains its promoter along
C.	The reporter gene is having a cloning site
D.	After insertion of DNA into the cloning site, selection of plasmids is carried out by blue white screening
Answer» B. It contains a reporter gene which contains its promoter along

Discussion

Explore topic-wise MCQs in Genetic Engineering.

Protein-protein interactions such as in electron transport lead to activation of the reporter gene.

_____ should enter the cell in the case of activation of the reporter gene for two hybrid system.

For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein.

Sequences that can function as origins of replication are called as ___________

In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened.

______SHOULD_ENTER_THE_CELL_IN_THE_CASE_OF_ACTIVATION_OF_REPORTER_GENE_FOR_TWO_HYBRID_SYSTEM.?$

Protein-protein interactions such as in electron transport lead to activation of reporter gene. Is the given statement true or false?$

The transcription domain is ________ if some of the bait and prey proteins are non-specific in nature?

For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein?

In two hybrid screening system, the activator binds through _ domain to a sequence upstream of the gene under its control, and __ domain stimulates transcription.

Screening is often carried out for a sequence that interacts with a protein for which already a clone is present. It is carried out in which host?

Sequences that can function as origins of replication are called as:

In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened. Is the given statement true or false?

Luciferase genes are also used at times for detection. Choose the correct statement for them.

GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.

Promoter-probe vectors are used often. Choose the correct statement for these vectors.

Explore topic-wise MCQs in Genetic Engineering.

Protein-protein interactions such as in electron transport lead to activation of the reporter gene.

_____ should enter the cell in the case of activation of the reporter gene for two hybrid system.

For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein.

Sequences that can function as origins of replication are called as ___________

In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened.

______SHOULD_ENTER_THE_CELL_IN_THE_CASE_OF_ACTIVATION_OF_REPORTER_GENE_FOR_TWO_HYBRID_SYSTEM.?$

Protein-protein interactions such as in electron transport lead to activation of reporter gene. Is the given statement true or false?$

The transcription domain is ________ if some of the bait and prey proteins are non-specific in nature?

For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein?

In two hybrid screening system, the activator binds through _______ domain to a sequence upstream of the gene under its control, and ________ domain stimulates transcription.

Screening is often carried out for a sequence that interacts with a protein for which already a clone is present. It is carried out in which host?

Sequences that can function as origins of replication are called as:

In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened. Is the given statement true or false?

Luciferase genes are also used at times for detection. Choose the correct statement for them.

GFP is one of a marker which is used for screening libraries in hosts other than E. coli. Choose the incorrect statement for GFP.

Promoter-probe vectors are used often. Choose the correct statement for these vectors.

In two hybrid screening system, the activator binds through _ domain to a sequence upstream of the gene under its control, and __ domain stimulates transcription.