MCQOPTIONS
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MCQOPTIONS
This section includes 17 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
What are the effects of increasing concentration of reaction components? |
| A. | It increases chances of ligation in both intramolecular and intermolecular reactions |
| B. | It increases chances of ligation only in intermolecular and no effect on intramolecular |
| C. | It decreases chances of ligation in intramolecular and increases in that of intermolecular |
| D. | It decreases chances of ligation in both types of reaction |
| Answer» C. It decreases chances of ligation in intramolecular and increases in that of intermolecular | |
| 2. |
What is the kinetics of the intramolecular and intermolecular ligation reactions? |
| A. | Second order kinetics for intramolecular and first order for intermolecular |
| B. | First order kinetics for intramolecular and second order for intermolecular |
| C. | Both are first order |
| D. | Both are second order |
| Answer» C. Both are first order | |
| 3. |
Ligation reaction can be both intramolecular and intermolecular in nature. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
In the case of blunt-end ligation, blunt ends can be generated by ___________ |
| A. | simply the action of restriction endonuclease which gives straight ends |
| B. | the polishing of staggered ends |
| C. | both the action of restriction endonuclease which gives straight ends and polishing of staggered ends |
| D. | by the action of restriction endonuclease which gives staggered ends |
| Answer» D. by the action of restriction endonuclease which gives staggered ends | |
| 5. |
If only one bond is broken in the sugar-phosphate backbone, it is called as ___________ |
| A. | gap |
| B. | nick |
| C. | break |
| D. | leakage |
| Answer» C. break | |
| 6. |
Ligation is defined as ______________ |
| A. | Alignment of only double stranded DNA molecules at the ends and the formation of phosphodiester bonds between both the strands |
| B. | Alignment of either of the double or single stranded DNA molecules and formation of glycosidic bonds between both the strands |
| C. | Alignment of either of the double or single stranded DNA molecules and the formation of phosphodiester bonds. The bond can be between either one or both the strands |
| D. | Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands |
| Answer» D. Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands | |
| 7. |
WHAT_IS_THE_KINETICS_OF_THE_INTRAMOLECULAR_AND_INTERMOLECULAR_LIGATION_REACTIONS??$ |
| A. | Second order kinetics for intramolecular and first order for intermolecular |
| B. | First order kinetics for intramolecular and second order for intermolecular |
| C. | Both are first order |
| D. | Both are second order |
| Answer» C. Both are first order | |
| 8. |
What are the effects of increasing concentration of reaction components?$ |
| A. | It increases chances of ligation in both intramolecular and intermolecular reactions |
| B. | It increases chances of ligation only in intermolecular and no effect on intramolecular |
| C. | It decreases chances of ligation in intramolecular and increase in that of intermolecular |
| D. | It decreases chances of ligation in both types of reaction |
| Answer» C. It decreases chances of ligation in intramolecular and increase in that of intermolecular | |
| 9. |
If a ligation reaction is being carried out and recircularization is observed, which type of reaction is being carried out? |
| A. | Intramolecular |
| B. | Intermolecular |
| C. | Both observe recircularization equally |
| D. | Recircurlization is not possible in any of the case |
| Answer» B. Intermolecular | |
| 10. |
Ligation reaction can be both intramolecular and intermolecular in nature. True or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 11. |
Why sticky ended ligations are carried out at temperatures lower than room temperature? |
| A. | It is so because the vibrational and kinetic energy of the molecules at room temperature is lower than that of the energy required to break the bonds holding the ends |
| B. | The energy required to break the bonds holding the ends is very less than that of the kinetic and vibrational energy at room temperature |
| C. | The enzyme carrying out ligation is unstable at low temperature |
| D. | The sticky ends created, don’t just religate at low temperature |
| Answer» B. The energy required to break the bonds holding the ends is very less than that of the kinetic and vibrational energy at room temperature | |
| 12. |
The sticky ends are held together by which type of bonds? |
| A. | Hydrogen bond |
| B. | Covalent bond |
| C. | Ionic bond |
| D. | Van-der-waal forces |
| Answer» B. Covalent bond | |
| 13. |
The ligation reaction is more efficient in which case? |
| A. | Blunt end ligation |
| B. | Sticky end ligation |
| C. | Both have same efficiency |
| D. | Depends on the reaction conditions |
| Answer» C. Both have same efficiency | |
| 14. |
In the case of blunt-end ligation, blunt ends can be generated by: |
| A. | simply the action of restriction endonuclease which gives straight ends |
| B. | the polishing of staggered ends |
| C. | both the action of restriction endonuclease which give straight ends and polishing of staggered ends |
| D. | by the action of restriction endonuclease which gives staggered ends |
| Answer» D. by the action of restriction endonuclease which gives staggered ends | |
| 15. |
How many categories of ligation reaction are there on the basis of ends created? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 16. |
If only one bond is broken in the sugar-phosphate backbone, it is called as: |
| A. | gap |
| B. | nick |
| C. | break |
| D. | leakage |
| Answer» C. break | |
| 17. |
Ligation is defined as: |
| A. | Alignment of only double stranded DNA molecules at the ends and the formation of phosphodiester bonds between both the strands |
| B. | Alignment of either of the double or single stranded DNA molecules and formation of glycosidic bonds between both the strands |
| C. | Alignment of either of the double or single stranded DNA molecules and then formation of phosphodiester bonds. The bond can be between either one or both the strands |
| D. | Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands |
| Answer» D. Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands | |