MCQOPTIONS
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MCQOPTIONS
This section includes 21 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the statement is incorrect for jumping PCR? |
| A. | It is used in the case when the DNA fragment is degraded |
| B. | In this type of PCR, the molecules are not long enough to span between the two primer sites |
| C. | At the end of first round of synthesis, the extension of the molecule from the primer site to the end of the fragmented molecule takes place |
| D. | It doesn’t leads to the formation of chimeric product |
| Answer» E. | |
| 2. |
Errors can be introduced because of many reasons such as polymerase error or because of heterogeneity. Which of the statement holds true? |
| A. | The error caused because of polymerase is biased for the first position in the codon |
| B. | The error caused because of polymerase is evenly distributed on all the positions in the codon |
| C. | The error caused by sequence heterogeneity is mainly because of first position in the codon |
| D. | The error caused by sequence heterogeneity is evenly distributed on all the codon positions |
| Answer» C. The error caused by sequence heterogeneity is mainly because of first position in the codon | |
| 3. |
Which of the statement is correct for misincorporation? |
| A. | If direct sequencing has carried this misincorporation is a great problem |
| B. | The erroneous molecules give strong signals than genuine molecules in case of misincorporation |
| C. | If the misincorporation in cloned PCR products it is a problem |
| D. | Even if the error is induced at an early stage it is not incorporated in many sequences |
| Answer» D. Even if the error is induced at an early stage it is not incorporated in many sequences | |
| 4. |
Heterogeneity can also arise if DNA is damaged before amplification. Which of the following doesn’t cause DNA damage? |
| A. | Amination of bases |
| B. | Chemical cross-linking between the strands |
| C. | Chemical cross-linking within the strands |
| D. | Slowing down the polymerase |
| Answer» B. Chemical cross-linking between the strands | |
| 5. |
If the template DNA belongs to several individual rather than single one, this type of heterogeneity is known as ____________ |
| A. | Heterzygosity |
| B. | Product heterogeneity |
| C. | Population heterogeneity |
| D. | Template heterogeneity |
| Answer» D. Template heterogeneity | |
| 6. |
During amplification, there are chances of having a product of a mixture of different sequences. There are various ways to detect it. Which of the statement is true in regard to it? |
| A. | Direct sequencing can’t be used in the case if the template DNA is heterozygous at the locus |
| B. | Direct sequencing can be used if the template DNA is heterozygous at the locus |
| C. | If cloning is done before sequencing, then it is detected via using only a single clone for sequencing |
| D. | In the case several recombinants are used, it can’t go undetected |
| Answer» C. If cloning is done before sequencing, then it is detected via using only a single clone for sequencing | |
| 7. |
There are basically two types of contamination, laboratory and external. If a PCR product is found to be contaminated by bacteria. It comes under laboratory contamination. |
| A. | True |
| B. | False |
| Answer» C. | |
| 8. |
How can the specificity of primer annealing be increased? |
| A. | Use of short primers |
| B. | Raising temperature |
| C. | Adjusting the concentration of sodium ions |
| D. | Using polymerase with proof reading activity |
| Answer» C. Adjusting the concentration of sodium ions | |
| 9. |
Which of the following conditions don’t contribute to wrong annealing to primer? |
| A. | Chance complementarity |
| B. | Conditions of annealing |
| C. | The original sequence of the primers |
| D. | Both the conditions of annealing and the original sequence don’t play any role |
| Answer» E. | |
| 10. |
WHICH_OF_THE_STATEMENT_IS_CORRECT_FOR_MISINCORPORATION??$ |
| A. | If direct sequencing is carried this misincorporation is a great problem |
| B. | The erroneous molecules give strong signals than genuine molecules in case of misincorporation |
| C. | If the misincorporation in cloned PCR products it is a problem |
| D. | Even if the error is induced at an early stage it is not incorporated in many sequences |
| Answer» D. Even if the error is induced at an early stage it is not incorporated in many sequences | |
| 11. |
Which of the statement is incorrect for jumping PCR?$ |
| A. | It is used in the case when the DNA fragment is degraded |
| B. | In this type of PCR, the molecules are not long enough to span between the two primer sites |
| C. | At the end of first round of synthesis, the extension of the molecule from the primer site to the end of the fragmented molecule takes place |
| D. | It doesn’t leads to the formation of chimeric product |
| Answer» E. | |
| 12. |
Errors can be introduced because of many reasons such as polymerase error or because of heterogeneity. Which of the statement holds true?$ |
| A. | The error caused because of polymerase is biased for the first position in the codon |
| B. | The error caused because of polymerase is evenly distributed on all the positions in the codon |
| C. | The error caused by sequence heterogeneity is mainly because of first position in the codon |
| D. | The error caused by sequence heterogeneity is evenly distributed on all the codon positions |
| Answer» C. The error caused by sequence heterogeneity is mainly because of first position in the codon | |
| 13. |
If cytosine is deaminated, which of the base is formed? |
| A. | Thymine |
| B. | Guanine |
| C. | Adenine |
| D. | Uracil |
| Answer» E. | |
| 14. |
Heterogeneity can also arise if DNA is damaged before amplification. Which of the following doesn’t cause DNA damage?# |
| A. | Amination of bases |
| B. | Chemical cross-linking between the strands |
| C. | Chemical cross-linking within the strands |
| D. | Slowing down the polymerase |
| Answer» B. Chemical cross-linking between the strands | |
| 15. |
If the template DNA belongs to several individual rather than single one, this type of heterogeneity is known as: |
| A. | Heterzygosity |
| B. | Product heterogeneity |
| C. | Population heterogeneity |
| D. | Template heterogeneity |
| Answer» D. Template heterogeneity | |
| 16. |
During amplification, there are chances of having a product of mixture of different sequences. There are various ways to detect it. Which of the statement is true in regard to it? |
| A. | Direct sequencing can’t be used in the case if the template DNA is heterozygous at the locus |
| B. | Direct sequencing can be used if the template DNA is heterozygous at the locus |
| C. | If cloning is done before sequencing, then it is detected via using only a single clone for sequencing |
| D. | In the case several recombinants are used, it can’t go undetected |
| Answer» C. If cloning is done before sequencing, then it is detected via using only a single clone for sequencing | |
| 17. |
Which can be used as a precaution in order to minimize contamination? |
| A. | Careful use and design of pipettes |
| B. | Placing the pre-PCR and post-PCR stages in same rooms |
| C. | Extracting the DNA along with surface layers |
| D. | Use of primers carefully is not very important |
| Answer» B. Placing the pre-PCR and post-PCR stages in same rooms | |
| 18. |
There are basically two types of contamination, laboratory and external. If a PCR product is found to be contaminated by bacteria. It comes under laboratory contamination. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» C. | |
| 19. |
How can specificity of primer annealing be increased? |
| A. | Use of short primers |
| B. | Raising temperature |
| C. | Adjusting the concentration of sodium ions |
| D. | Using polymerase with proof reading activity |
| Answer» C. Adjusting the concentration of sodium ions | |
| 20. |
Which of the following conditions don’t contribute to wrong annealing to primer?$ |
| A. | Chance complementarity |
| B. | Conditions of annealing |
| C. | The original sequence of the primers |
| D. | Both the conditions of annealing and the original sequence don’t play any role |
| Answer» E. | |
| 21. |
Which of the statement holds for long-range PCR and in its relation? |
| A. | It is the PCR in which longer templates are used |
| B. | DNA polymerases which don’t have proof-reading activity give larger products |
| C. | DNA polymerases’ processivity is not a measure to have larger products |
| D. | It is PCR in which a mixture of enzymes is used to have larger products |
| Answer» E. | |