MCQOPTIONS
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MCQOPTIONS
| 1. |
Why sticky ended ligations are carried out at temperatures lower than room temperature? |
| A. | It is so because the vibrational and kinetic energy of the molecules at room temperature is lower than that of the energy required to break the bonds holding the ends |
| B. | The energy required to break the bonds holding the ends is very less than that of the kinetic and vibrational energy at room temperature |
| C. | The enzyme carrying out ligation is unstable at low temperature |
| D. | The sticky ends created, don’t just religate at low temperature |
| Answer» B. The energy required to break the bonds holding the ends is very less than that of the kinetic and vibrational energy at room temperature | |