MCQOPTIONS
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MCQOPTIONS
This section includes 197 Mcqs, each offering curated multiple-choice questions to sharpen your Advanced Biotech knowledge and support exam preparation. Choose a topic below to get started.
| 51. |
Plant breeders have an advantage over animal breeders in reproducing a desired type offspring because the plant breeders can employ |
| A. | Gene mutations |
| B. | Hybridization |
| C. | Clonal propagation |
| D. | Selection |
| Answer» D. Selection | |
| 52. |
Match the following marker genes in group 1 with suitable selecting agent in group 2 |
| A. | P-1, Q-2, R-4, S-3 |
| B. | P-3, Q-2, R-4, S-1 |
| C. | P-2, Q-3, R-4, S-1 |
| D. | P-3, Q-1, R-4, S-2 |
| Answer» E. | |
| 53. |
Gynogenesis is a process of development of haploid plants |
| A. | from a fertilized cell of female gametophyte |
| B. | from an unfertilized cell of female gametophyte |
| C. | from isolated pollen grains |
| D. | by selective elimination of chromosomes following distant hybridization |
| Answer» C. from isolated pollen grains | |
| 54. |
Oils rich in PUFA are NOT desirable for bio-diesel production because |
| A. | they form epoxides in presence of oxygen |
| B. | they do not form epoxides in presence of oxygen |
| C. | they have high ignition temperature |
| D. | they solidify at low temperature |
| Answer» B. they do not form epoxides in presence of oxygen | |
| 55. |
Plant secondary metabolites |
| A. | help to increase the growth rate of plant |
| B. | help in plant reproduction processes |
| C. | provide defense mechanisms against microbial attack |
| D. | make the plant susceptible to unfavorable conditions |
| Answer» D. make the plant susceptible to unfavorable conditions | |
| 56. |
For amplificatio of GC rich sequences by polymerase chain reacstion, identify the reagent that binds and stabilizes AT sequences and destabilizes GC regions. |
| A. | Tetramethyl ammonium chloride |
| B. | Betaine |
| C. | 7-deaza-2 -deoxyguanosine triphosphate |
| D. | Sodium dodecyl sulphate |
| Answer» C. 7-deaza-2 -deoxyguanosine triphosphate | |
| 57. |
Protein binding regions of DNA are identified by one of the following techniques |
| A. | finger printing |
| B. | foot printing |
| C. | southern blotting |
| D. | western blotting |
| Answer» C. southern blotting | |
| 58. |
To promote attachment and spreading of anchorage-dependent animal cells, the surface of the culture vessel needs to be coated with |
| A. | trypsin |
| B. | collagen |
| C. | pronase |
| D. | polyglycol |
| Answer» C. pronase | |
| 59. |
When present in tissue culture medium, gibberellin |
| A. | helps to break dormancy of buds and bulbs |
| B. | promotes dormancy development in buds and bulbs |
| C. | is regarded as plant growth inhibitor |
| D. | prevents normal recognition of auxin molecule |
| Answer» B. promotes dormancy development in buds and bulbs | |
| 60. |
A thermostable DNA polymerase that can carry out both reverse transcription reaction and polymerizations has been isolated from |
| A. | Thermococcus litoralis |
| B. | Thermus aquaticus |
| C. | Thermotoga maritima |
| D. | Thermus thermophilus |
| Answer» E. | |
| 61. |
Meristems escape virus invasion because |
| A. | vascular system is absent in the meristem |
| B. | of low metabolic activity in the meristem |
| C. | the virus inactivating system has low activity in the meristem |
| D. | of low endogenous auxin level |
| Answer» B. of low metabolic activity in the meristem | |
| 62. |
If Con A sepharose column was used for the purification of enzyme, then separation would be based on |
| A. | molecular exclusion |
| B. | affinity binding |
| C. | ion exchange |
| D. | hydrophobic interaction |
| Answer» C. ion exchange | |
| 63. |
What techniques would you recommend for confirming the molecular weight of the purified enzyme? |
| A. | P, Q |
| B. | Q, S |
| C. | R, S |
| D. | P, S |
| Answer» C. R, S | |
| 64. |
Which of the following would result in somaclonal variation in micropropagated plants? |
| A. | P, Q |
| B. | Q, R |
| C. | P, S |
| D. | Q, S |
| Answer» C. P, S | |
| 65. |
The mobility of DNA in agarose gel electrophoresis is solely based on its |
| A. | charge |
| B. | conformation |
| C. | size |
| D. | none of these |
| Answer» D. none of these | |
| 66. |
The transplastomic plants bear no risk for gene transfer through pollens as |
| A. | the pollens degenerate before fertilization |
| B. | the transformed mitochondrial DNA is lost during pollen maturation |
| C. | the transformed chloroplast DNA is lost during pollen maturation |
| D. | the transformed genomic DNA are inherited maternally |
| Answer» E. | |
| 67. |
Cells of meristemoid are best described as |
| A. | differentiated and non dividing |
| B. | dedifferentiated and dividing |
| C. | differentiated and dividing |
| D. | dedifferentiated and non dividing |
| Answer» D. dedifferentiated and non dividing | |
| 68. |
Identical sized RNA transcript is detected by Northern blot analysis of UDP glucuronosyl transferase obtained from human liver and kidney. Microarray analysis of the same samples shows equal spot intensity, ohereas Western blot detects a 55 kDa strong band in liver, but a very faint band in kidney of same size. The regulation of UDP glucuronosyl transferase is |
| A. | transcriptionally controlled |
| B. | post-transcriptionally controlled |
| C. | translationally controlled |
| D. | post-translationally controlled |
| Answer» C. translationally controlled | |
| 69. |
Though the right border (RB) and left border (LB) of T-DNA are identical, the DNA transfer is specific for the DNA left of the RB (the T-DNA), rather than for the DNA left of the LB because |
| A. | the sequence context at the RB defines the direction of transfer |
| B. | the sequence context at the LB defines the direction of transfer |
| C. | the nuclear location sequence (NLS) of VirD2 protein drives the excised T-strand |
| D. | the endonuclease activity of VirD2 protein allows nicking at RB |
| Answer» B. the sequence context at the LB defines the direction of transfer | |
| 70. |
Somatic embryo from cotyledon explant would develop in the following sequential stages, |
| A. | cotyledonary heart globular torpedo |
| B. | globular torpedo heart cotyledonary |
| C. | globular heart torpedo cotyledonary |
| D. | cotyledonary globular heart torpedo |
| Answer» D. cotyledonary globular heart torpedo | |
| 71. |
A linear DNA fragment is 100% labeled at one end and has 3 restriction sites for EcoRI. If it is partially digested by EcoRI so that all possible fragments are produced, how many of these fragments will be labeled and how many will not be labeled ? |
| A. | 4 labeled; 6 unlabeled |
| B. | 4 labeled; 4 unlabeled |
| C. | 3 labeled; 5 unlabeled |
| D. | 3 labeled; 3 unlabeled |
| Answer» D. 3 labeled; 3 unlabeled | |
| 72. |
Which of the following fluorescent probes is used to monitor the progress of ampli fication in Real time PCR ? |
| A. | SYBR green |
| B. | Rhodamine |
| C. | FITC |
| D. | Cyan blue |
| Answer» B. Rhodamine | |
| 73. |
Agrobacterium based transformation of protoplasts obtained from dicots is based on the fact that |
| A. | These exhibit strong chromosomal structures |
| B. | These have two cotyledons |
| C. | These exhibit strong wound response |
| D. | These have long tap root system |
| Answer» D. These have long tap root system | |
| 74. |
For the sequence of ds DNA given below, identify the set of primers required to amplity this DNA by PCR |
| A. | 5 GGTTGTA and 5 GACTCCA |
| B. | 5 CTGAGGT and 5 CCCAACAT |
| C. | 5 ACTCAGT and 5 ATGTTGG |
| D. | None of these |
| Answer» B. 5 CTGAGGT and 5 CCCAACAT | |
| 75. |
The restriction endonuclease HaeIII recognizes the sequence GG CC and the point of cleavage is given by the arrow. If you want to clone a piece of DNA in a plasmid digested by HaeIII, what will be restriction enzyme of choice ? |
| A. | SmaI (CC GGG) |
| B. | NotI(GC GGCCGC) |
| C. | SalmIII (GG CC) |
| D. | PstI (CTGCA G) |
| Answer» D. PstI (CTGCA G) | |
| 76. |
Tobacco leaf discs are transfected with Agrobacterium tumefaciens strain containing binary vector (GUS as reporter gene) with selectable marker neo (kanamycin resistant gene) and then regenerated to plants. The plants are kanamycin resistant but leaf tissues are negative to GUS assay. The explanations are |
| A. | The plants are transformed for both genes by GUS gene is turned off |
| B. | The plants are transformed for only neo gene not the GUS gene |
| C. | The plants are not transformed at all, but the development of kanamycin resistance is due to somaclonal variation |
| D. | All of these |
| Answer» D. All of these | |
| 77. |
The culture fluids of 1000 to 5000 colonies of hybridoma are screened for monoclonal antibody by |
| A. | P, Q |
| B. | Q, R |
| C. | R, S |
| D. | Q, S |
| Answer» B. Q, R | |
| 78. |
Expression of hundreds of different genes in DNA microarray technology is monitored by using |
| A. | Radioactive probe |
| B. | Visible chromogenic probe |
| C. | UV absorbing probe |
| D. | Fluorescent probe |
| Answer» E. | |
| 79. |
Which of the following types of cancer will be observed in such transformed cells ? |
| A. | Adenoma |
| B. | Melanoma |
| C. | Sarcoma |
| D. | Hepatoma |
| Answer» D. Hepatoma | |
| 80. |
Western blot analysis of c-myc expression of such transformed cells last for |
| A. | transiently |
| B. | upto five generations |
| C. | upto 10 generations |
| D. | more than 100 generations |
| Answer» D. more than 100 generations | |
| 81. |
The DNA smear obtained on Southern blot is due to |
| A. | head to head concatamer of viral DNA |
| B. | head to tail concatamer of viral DNA |
| C. | tail to tail concatamer of viral DNA |
| D. | random integration of viral DNA |
| Answer» C. tail to tail concatamer of viral DNA | |
| 82. |
Expression in poor amount and in inactive form of cDNA of a euharyotic protein in Escherichia coli using its expression vector is due to |
| A. | P, Q |
| B. | Q, R |
| C. | Q, S |
| D. | P, S |
| Answer» E. | |
| 83. |
The following are useful to introduce genes into crop plants except |
| A. | Ti plasmid |
| B. | Particle gun |
| C. | breeding |
| D. | auxin |
| Answer» E. | |
| 84. |
For protoplast fusion to be successful in plant cells |
| A. | fusion agents other than polyethylene glycol should be used |
| B. | cell wall of the two strains of cells should not be damaged |
| C. | DNA between the two cells should be compatible |
| D. | osmolarity of the medium is not important |
| Answer» D. osmolarity of the medium is not important | |
| 85. |
You have cut the genome of a double-stranded viral genome with a restriction endonuclease and electrophoresed the products on an agarose gel. You observe only one band on the gel, equivalent to the size of the genome. This is because |
| A. | there are no introns in the genome |
| B. | the introns contain the recognition sites and have already been spliced out |
| C. | all of restriction fragments are too small to detect |
| D. | restriction endonucleases do not cut RNA, and this virus has an RNA genome |
| Answer» E. | |
| 86. |
All of the following are true about DNA microarray technology except |
| A. | an electron microscope is used to gather data from the arrays |
| B. | the technology is used to assess transcription from multiple genes simultaneously |
| C. | the technology works best for organisms whose genome is completely sequenced |
| D. | the technology is derived from computer chip manufacture |
| Answer» B. the technology is used to assess transcription from multiple genes simultaneously | |
| 87. |
To generate the minimum number of subclones needed for sequencing, what should be the size of the insert in these subclones ? |
| A. | 1000 bp |
| B. | 500 bp |
| C. | 250 bp |
| D. | 2000 bp |
| Answer» C. 250 bp | |
| 88. |
Which one of the following will be the most appropriate restriction enzyme for this subcloning? |
| A. | 8-bp cutter |
| B. | 6-bp cutter |
| C. | 3-bp cutter |
| D. | 5-bp cutter |
| Answer» D. 5-bp cutter | |
| 89. |
In order to identify the person who committed a crime, forensic experts will need to extract DNA |
| A. | cut the DNA and hybridize with specific micro-satellite probes |
| B. | cut the DNA and subclone the fragments |
| C. | determine the sequence of the subclones |
| D. | (b) followed by (c) |
| Answer» B. cut the DNA and subclone the fragments | |
| 90. |
All of the following produced by animal cells in culture and help the cells adhere to the culture dish except |
| A. | glycoproteins |
| B. | collagen |
| C. | phospholipase A |
| D. | hyaluronic acid |
| Answer» D. hyaluronic acid | |
| 91. |
What is the primary purpose of neomycin in creating mice with knock-outs in gene X? |
| A. | neomycin selects for the survival of embryonic stem cells (ES) that have incorporated the mutant gene X anywhere in the genome |
| B. | neomycin selects for the survival of ES cells that have incorporated the mutant gene in the place of the wild-type gene |
| C. | neomycin prevents Candida infection during ES cell culture that does not have gene X |
| D. | neomycin makes the gene X knock - out mice resistant to Candida infection |
| Answer» B. neomycin selects for the survival of ES cells that have incorporated the mutant gene in the place of the wild-type gene | |
| 92. |
If bacterial cells are transformed with a mixture of linear and circular molecules resulting from a ligation reaction designed to produce a recombinant molecule |
| A. | no recombinant molecule will ever be detected |
| B. | both linear and circular molecules will replicate equally well |
| C. | none of the plasmids will express the antibiotic resistance gene located on the plasmid |
| D. | the circular molecules will be amplified by the cells |
| Answer» E. | |
| 93. |
The restriction endonuclease Eco 52I recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco 52I ? |
| A. | EcoRI (G/AATTC) |
| B. | XmaIII (C/GGCGG) |
| C. | SmaI (CCC/GGG) |
| D. | SacII (CCGC/GG) |
| Answer» C. SmaI (CCC/GGG) | |
| 94. |
In baculovirus expression vector foreign genes are expressed from the promoter of |
| A. | Polyhedron gene |
| B. | Bacteriophage T7 gene |
| C. | E. coli lacZ gene |
| D. | Yeast phosphoglycerate kinase gene |
| Answer» B. Bacteriophage T7 gene | |
| 95. |
Plant secondary metabolites production in suspension culture is mainly targeted for |
| A. | Obtaining metabolites in aseptic condition |
| B. | Enhanced in vitro production of desired metabolite |
| C. | Enhanced production of all metabolites |
| D. | Obtaining new metabolites |
| Answer» C. Enhanced production of all metabolites | |
| 96. |
T4 Polynucleotide kinase is used for |
| A. | Labelling 3 ends of DNA |
| B. | Labelling 5 ends of DNA |
| C. | Creating blunt ends of DNA |
| D. | Dephosphorylation of DNA |
| Answer» C. Creating blunt ends of DNA | |
| 97. |
Cells deficient in hypoxanthine guanine phosphoribosyl transferase (HPRT) enzyme rely on |
| A. | Synthesis of purine deoxynucleotides by salvage pathway |
| B. | Synthesis of purine deoxynucleotides by de novo pathway |
| C. | Supply of hypoxanthine in the culture medium |
| D. | Supply of thymidine in the culture medium |
| Answer» C. Supply of hypoxanthine in the culture medium | |
| 98. |
Enzyme used in cycle sequencing of DNA is |
| A. | T7 DNA polymerase |
| B. | T4 DNA polymerase |
| C. | Klenow DNA polymerase |
| D. | Taq DNA polymerase |
| Answer» E. | |
| 99. |
The length of each boarder sequence in Ti -plasmid is about |
| A. | 25 million base pairs |
| B. | 200 kilo base pairs |
| C. | 25 kilo base pairs |
| D. | 25 base pairs |
| Answer» E. | |
| 100. |
Hormone pairs required for a callus to differentiate are |
| A. | Auxin and cytokinin |
| B. | Auxin and gibberellin |
| C. | Ethylene and gibberellin |
| D. | Cytokinin and gibberellin |
| Answer» B. Auxin and gibberellin | |