The restriction endonuclease HaeIII recognizes the sequence GG CC and the point of cleavage is given by the arrow. If you want to clone a piece of DNA in a plasmid digested by HaeIII, what will be restriction enzyme of choice ?

The restriction endonuclease HaeIII recognizes the sequence GG CC an

1.	The restriction endonuclease HaeIII recognizes the sequence GG CC and the point of cleavage is given by the arrow. If you want to clone a piece of DNA in a plasmid digested by HaeIII, what will be restriction enzyme of choice ?
A.	SmaI (CC GGG)
B.	NotI(GC GGCCGC)
C.	SalmIII (GG CC)
D.	PstI (CTGCA G)
Answer» D. PstI (CTGCA G)

Discussion

No Comment Found

Related MCQs

Which one of the following is not a requirement for a PCR reaction ?
To PCR amply the sequence ATCTTCTACG...............AAGCTTGCGG TAGAAGATGC...............TTCGAACGCC the required primers are
In nature, Agrobacterium tumefaciens mediated infection of plant cells leads to P. crown gall disease in plants Q. hairy root disease in plants R. transfer of T-DNA into the plant chromosome S. transfer of Ri-plasmid into the plant cell
The number of clones to represent this fragment in genomic library with a probability of 95% are
A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRIindividually. Restriction digestion yielded following fragments. Plasmid + HindIII 1200bp and3800bp Plasmid + BamHI 5000bp Plasmid + EcoRI 2500bp The number of sites for EcoRI, BamHI-andHindIIIpresent on this plasmid are
Match the herbicides in Group I with the target enzymesin Group II. Group I Group II P. Glyphosate 1. Nitrilase Q. Bromoxynil 2. Acetolactatesyn thetase R. Sulphonylureas 3. Dehalogenase S. Dalapon 4. 5-Enolpyruvylshikimate 3-phosphate synthase
Determine the correctness or otherwise of the following Assertion (a) and Reason (r). Assertion : The production of secondary metabolites in plant cell cultures is enhanced by the addition of elicitors. Reason : Elicitors induce the expression of enzymes responsibie for the biosynthesis of secondary metabolites.
A single base pair of DNA weighs 1.1 10 21 grains. How many picomoles of a plasmid vector of length 2750 bp are contained in 1 ug of purified DNA?
Which one of the following is NOT a protoplast fusion inducing agent?
Protein-protein interactions are studied by P. DNA foot printing Q. Yeast two hybrid system R. Ligase chain reaction S. Mass spectrometry

The restriction endonuclease HaeIII recognizes the sequence GG CC and the point of cleavage is given by the arrow. If you want to clone a piece of DNA in a plasmid digested by HaeIII, what will be restriction enzyme of choice ?

Discussion

No Comment Found

Related MCQs

Reply to Comment