MCQOPTIONS
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MCQOPTIONS
This section includes 197 Mcqs, each offering curated multiple-choice questions to sharpen your Advanced Biotech knowledge and support exam preparation. Choose a topic below to get started.
| 101. |
Positional cloning approach exploits information |
| A. | On the location of the gene in the genome |
| B. | On the status of its expression |
| C. | About the position of promoter relative to MCS |
| D. | About the position of the restriction sites |
| Answer» B. On the status of its expression | |
| 102. |
In human populations, 4% of the individuals are homozygous recessive to a specific RFLP marker. What percentage of individuals do you expect to be heterozygous for this trait ? |
| A. | 4% |
| B. | 8% |
| C. | 16% |
| D. | 32% |
| Answer» E. | |
| 103. |
The essential component of Ti plasmid required for integration into plant genome is |
| A. | Origin of replication |
| B. | Tumor inducing gene |
| C. | Nopaline utilization gene |
| D. | All of these |
| Answer» C. Nopaline utilization gene | |
| 104. |
DNA helicases catalyse the reaction |
| A. | DNA supercoiling |
| B. | DNA unwinding |
| C. | Cleavage of DNA helix |
| D. | Interconversion of DNA topoisomers |
| Answer» C. Cleavage of DNA helix | |
| 105. |
The substrate for restriction enzyme is |
| A. | Single stranded RNA |
| B. | Partially double stranded RNA |
| C. | Cell wall proteins |
| D. | Double stranded DNA |
| Answer» E. | |
| 106. |
Embryo rescue is a useful technique to |
| A. | Grow/generate hybrids between different plant species |
| B. | Complete the growth of embryos susceptible to defects in seed development |
| C. | Break the dormancy of seeds |
| D. | All of the above |
| Answer» E. | |
| 107. |
A gene can not be isolated from a human genomic DNA library by functional complementation in E. coli because of |
| A. | Non-functional promoter |
| B. | The absence of splicing machinery |
| C. | Coupled transcription and translation |
| D. | Codon bias |
| Answer» C. Coupled transcription and translation | |
| 108. |
Yeast artificial chromosome (YAC) is used for |
| A. | cloning large segments of DNA |
| B. | cloning only, yeast genomic sequences |
| C. | cloning of only cDNA sequences |
| D. | all DNA except plant DNA sequences |
| Answer» B. cloning only, yeast genomic sequences | |
| 109. |
Phage T7 promoter containing plasmids are used for overexpression of cloned genes because |
| A. | their convenient size |
| B. | their single stranded nature |
| C. | Exquisite specificity of T7 RNA polymerase to phage promoters |
| D. | T7 infects E. coli and lysogenizes the cell |
| Answer» D. T7 infects E. coli and lysogenizes the cell | |
| 110. |
Which of the following has been produced commercially from mammalian cell cultures? |
| A. | Plasminogen activator |
| B. | Antibacterial antibiotics |
| C. | Insulin |
| D. | Renin |
| Answer» B. Antibacterial antibiotics | |
| 111. |
Mung bean nuclease could be used for |
| A. | DNA synthesis |
| B. | nucleotide hydrolysis |
| C. | trimming single stranded regions in DNA |
| D. | removal of phosphate groups from the ends of the DNA |
| Answer» D. removal of phosphate groups from the ends of the DNA | |
| 112. |
Many plasmids have Ampr marker. This implies |
| A. | The plasmids contain genes for ampicillin biosynthesis |
| B. | Ampicillin is required for bacterial growth after transformation |
| C. | The plasmid contains the gene encoding -lactamase |
| D. | Ampicillin is essential for cell survival |
| Answer» D. Ampicillin is essential for cell survival | |
| 113. |
Majority of the cereals are highly recalcitrant to Agrobacterium-mediated transformation, and so direct transfor-mation methods have been developed to transform such plants. Which of the following direct transformation methods is applicable to intact plant tissues? |
| A. | Calcium chloride and PEG-mediated transformation |
| B. | Liposome-mediated transformation |
| C. | Electroporation |
| D. | Transformation using microprojectiles |
| Answer» C. Electroporation | |
| 114. |
What are the experimental steps needed for screening an expression library for a clone encoding a protein X that has been isolated and purified ? |
| A. | P, S |
| B. | Q, S |
| C. | Q, R |
| D. | R, S |
| Answer» C. Q, R | |
| 115. |
Identify the natural plant growth regulators from the following list. |
| A. | P, Q |
| B. | Q, S |
| C. | P, R |
| D. | R, S |
| Answer» D. R, S | |
| 116. |
All the cells that participate in immune responses originate from a population of |
| A. | neutrophils |
| B. | stem cells |
| C. | macrophages |
| D. | lymphocytes |
| Answer» E. | |
| 117. |
Which of the following statements is INCORRECT about immobilized plant cell cultures? |
| A. | It is possible to use high cell densities |
| B. | Cells remain active for long periods |
| C. | Cell products or inhibitors can be removed easily |
| D. | It provides low shear resistance to cells |
| Answer» C. Cell products or inhibitors can be removed easily | |
| 118. |
To isolate a gene coding for glucagon, the cDNA library has to be constructed using mRNA isolated from |
| A. | Intestine |
| B. | Pancreas |
| C. | Pituitary |
| D. | Brain |
| Answer» C. Pituitary | |
| 119. |
Resistance to herbicide chlorsulfuron in plants is due to a change in |
| A. | Glutamine synthetase |
| B. | Threonine deaminase |
| C. | Acetolactate synthase |
| D. | DNA polymerase |
| Answer» D. DNA polymerase | |
| 120. |
Large scale clonal propagation practically means raising a population of plantlets from |
| A. | A single cell |
| B. | A single explant |
| C. | Many explants from a single plant |
| D. | Many explants from a group of plants |
| Answer» D. Many explants from a group of plants | |
| 121. |
Callus formation from mature tissue explant occurs through |
| A. | Dedifferentiation |
| B. | Redifferentiation |
| C. | Both (a) and (b) of the above |
| D. | None of (a) and (b) |
| Answer» D. None of (a) and (b) | |
| 122. |
To PCR amply the sequence |
| A. | ATCTTCTA and CGAACGCC |
| B. | ATCTTCTA and CCGCAAGC |
| C. | TAGAAGAT and CGAACGCC |
| D. | TAGAAGAT and CCGCAAGC |
| Answer» C. TAGAAGAT and CGAACGCC | |
| 123. |
Enhanced axillary branching for multiple shoot productions is promoted by |
| A. | 2, 4 D |
| B. | Abscisic acid |
| C. | Gibberellic acid |
| D. | Benzyl adenine |
| Answer» B. Abscisic acid | |
| 124. |
What would be the effect of addition of 2,4-D on the production of berberine by cell culture of Thalictrum minus |
| A. | To stimulate growth and thereby urease secondary metabolite production |
| B. | Stimulate dedifferentiation and thereby decrease secondary metabolite production |
| C. | Stimulate proliferation and reduce secondary metabolite production |
| D. | None of these |
| Answer» E. | |
| 125. |
In animal cell cultures, the addition of serum to media is essential for providing |
| A. | amino acids for protein synthesis |
| B. | nucleotides for DNA synthesis |
| C. | growth factors |
| D. | all of these |
| Answer» E. | |
| 126. |
To be a cloning vector, a plasmid does NOT require |
| A. | an origin of replication |
| B. | an antibiotic resistance marker |
| C. | a restriction site |
| D. | to have a high copy number |
| Answer» C. a restriction site | |
| 127. |
Match the following genetic elements with their functions. |
| A. | A 4, B 2, C 1, D 3 |
| B. | A 2, B 4, C 1, D 3 |
| C. | A 1, B 4, C 2, D 3 |
| D. | A 4, B 2, C 3, D 1 |
| Answer» B. A 2, B 4, C 1, D 3 | |
| 128. |
Somatic embryogenesis is a procedure in plant tissue culture methodology described best as |
| A. | Formation of both shoot and root meristem |
| B. | Formation of stable embryos |
| C. | Formation of axillary buds |
| D. | None of the above |
| Answer» E. | |
| 129. |
During the functioning of biosensor which of the following sequences of events occurs |
| A. | Enzymatic/cellular reaction detector transducer |
| B. | Enzymatic/cellular reaction transducer detector |
| C. | Enzymatic/cellular reaction pressure gauge time |
| D. | Enzymatic/cellular reaction vibrator mechanical signal |
| Answer» C. Enzymatic/cellular reaction pressure gauge time | |
| 130. |
Nick translation of DNA is a method for making DNA probes. Identify from below what is NOT required for nick translation method |
| A. | DNA polymerase |
| B. | DNAase |
| C. | Primers |
| D. | Deoxyribonucleotides |
| Answer» D. Deoxyribonucleotides | |
| 131. |
For the growth of T-cell, the growth factor needed would be |
| A. | Epidermal growth factor |
| B. | Interleukin-2 |
| C. | Fibroblast growth factor |
| D. | TNF- |
| Answer» C. Fibroblast growth factor | |
| 132. |
Transfer of T - DNA from Ti plasmid into plant cell is mediated by |
| A. | mob gene |
| B. | vir gene |
| C. | nif gene |
| D. | octopine gene |
| Answer» C. nif gene | |
| 133. |
Match items in group 1 with correct options from those given in group 2 |
| A. | P 4, Q 3, R l, S 2 |
| B. | P l, Q 3, R 4, S 2 |
| C. | P 3, Q l, R 2, S 4 |
| D. | P 3, Q l, R 4, S 2 |
| Answer» E. | |
| 134. |
Which of the following reagents is used for harvesting anchorage-dependent animal cells from culture vessels ? |
| A. | Trypsin/Collagensase |
| B. | Trypsin/Collagen |
| C. | Collagen/Fibronectin |
| D. | DMSO |
| Answer» B. Trypsin/Collagen | |
| 135. |
Which of the following statements are true with respect to a special complex called dicer ? |
| A. | P, R |
| B. | Q, R |
| C. | P, S |
| D. | Q, S |
| Answer» C. P, S | |
| 136. |
Match the items in groupl with the terms given in group 2 |
| A. | P- 2 , Q- 4 , R-1 , S- 3 |
| B. | P- 3 , Q- 4 , R-1 , S- 2 |
| C. | P- 4 , Q- 1 , R-2 , S- 3 |
| D. | P- 1 , Q- 3 , R-4 , S- 2 |
| Answer» B. P- 3 , Q- 4 , R-1 , S- 2 | |
| 137. |
Determine the correctness or otherwise of following Assertion [a] and Reason [r] |
| A. | Both [a] and [r] are true and [r] is the correct reason for [a] |
| B. | Both [a] and [r] are true but [r] is not the correct reason for [a] |
| C. | [a] is true but [r] is false |
| D. | [a] is false but [r] is true |
| Answer» C. [a] is true but [r] is false | |
| 138. |
Identify the correct statement for the HAT medium |
| A. | P, Q |
| B. | P, S |
| C. | R, S |
| D. | Q, S |
| Answer» C. R, S | |
| 139. |
Multiplication of genetically identical copies of a cultivar by asexual reproduction is known as |
| A. | aclonal propagation |
| B. | vegetative propagation |
| C. | polyclonal propagation |
| D. | clonal propagation |
| Answer» E. | |
| 140. |
Parthenogenetic embryos in plants are those which are formed by |
| A. | unfertilized eggs |
| B. | fertilized eggs |
| C. | sporophytic cells |
| D. | male gametophyte |
| Answer» B. fertilized eggs | |
| 141. |
A RNA polymerization assay was performed using |
| A. | 15 nmol |
| B. | 105 nmol |
| C. | 150 nmol |
| D. | 50 nmol |
| E. | None of the above |
| Answer» F. | |
| 142. |
Determine the correctness or otherwise of the following Assertion (a) and the Reason (r) |
| A. | both (a) and (r) are true and (r) is the correct reason for (a) |
| B. | both (a) and (r) are true and (r) is not the correct reason for (a) |
| C. | (a) is true but (r) is false |
| D. | (a) is false but (r) is true |
| Answer» D. (a) is false but (r) is true | |
| 143. |
Hydrated synthetic seeds which are produced by ion exchange reaction involve mixing the somatic embryos in a solution of |
| A. | sodium alginate and dropping it in a solution of calcium nitrate |
| B. | calcium alginate and dropping it in a solution of sodium nitrate |
| C. | calcium alginate and dropping it in a solution of ammonium nitrate |
| D. | mannitol and dropping it in a solution of sodium nitrate |
| Answer» B. calcium alginate and dropping it in a solution of sodium nitrate | |
| 144. |
Match the products in Group 1 with their possible applications in Group 2: |
| A. | P-3, Q-1, R-4, S-2 |
| B. | P-3, Q-4, R-1, S-2 |
| C. | P-2, Q-3, R-1, S-4 |
| D. | P-2, Q-1, R-4, S-3 |
| Answer» C. P-2, Q-3, R-1, S-4 | |
| 145. |
Protein-protein interactions are studied by |
| A. | P and S only |
| B. | Q and S only |
| C. | P and R only |
| D. | Q and R only |
| Answer» C. P and R only | |
| 146. |
The study of evolutionary relationships is known as |
| A. | genomics |
| B. | proteomics |
| C. | phylogenetics |
| D. | genetics |
| Answer» D. genetics | |
| 147. |
Restriction endonucleases which recognize and cut same recognition sequences are known as |
| A. | isoschizomers |
| B. | isozymes |
| C. | isoaccepting endonucleases |
| D. | abzymes |
| Answer» B. isozymes | |
| 148. |
Shoot organogenesis by tissue culture results into |
| A. | a bipolar structure that has no vascular connection with the explant |
| B. | a monopolar structure that has a strong connection with the pre-existing vascular tissue of the explant |
| C. | a monopolar structure that has no vascular connection with the explant |
| D. | a bipolar structure that has a strong connection with the pre-existing vascular tissue of the explant |
| Answer» C. a monopolar structure that has no vascular connection with the explant | |
| 149. |
Hairy roots induced in vitro by the infection of Agrobacterium rhizogenes, are characterized by |
| A. | P and R only |
| B. | P and Q only |
| C. | Q and R only |
| D. | R and S only |
| Answer» B. P and Q only | |
| 150. |
The bacteria known to be naturally competent for transformation of DNA is |
| A. | Escherichia coli |
| B. | Bacillus subtilis |
| C. | Mycobacterium tuberculosis |
| D. | Yersinia pestis |
| Answer» B. Bacillus subtilis | |