The restriction endonuclease Eco 52I recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco 52I ?

The restriction endonuclease Eco 52I recognizes the sequence C/GGCGG a

1.	The restriction endonuclease Eco 52I recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco 52I ?
A.	EcoRI (G/AATTC)
B.	XmaIII (C/GGCGG)
C.	SmaI (CCC/GGG)
D.	SacII (CCGC/GG)
Answer» C. SmaI (CCC/GGG)

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The restriction endonuclease Eco 52I recognizes the sequence C/GGCGG and cuts between the first C and the first G, indicated by the slash. DNA cut by which of the following enzymes (given with their recognition sequences and cut sites) could be cloned into a plasmid digested with Eco 52I ?

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