Which of the given statements is incorrect about the Comparison of proteomes to EST databases of an organism?

• A. ESTs are single DNA sequence reads that contain a small fraction of incorrect base assessments, insertions, and deletions
• B. Many sequences arise from near the 5 end of the mRNA, although every effort is usually made to read as far 3 as possible into the upstream portion of the cDNA
• C. EST libraries are useful for preliminary identification of genes by database similarity searches
• D. An EST database of an organism can be analyzed for the presence of gene families,
• E. northologs, and paralogs