1.

Which of the following is not included as a step in cloning in bacteriophage PI?

A. The growth of vector is done as a plasmid by using pBR322 origin of replication
B. Cleaving with BamH1 is done and then products cleaved with Sau3A are ligated
C. Incubation with mutant lysogen is done which doesn t cleaves the recombinants at pac sites
D. Incubation is done with mutant lysogen which doesn t contains head and tail proteins
Answer» D. Incubation is done with mutant lysogen which doesn t contains head and tail proteins


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