What will happen if the amino acid sequence is used directly for primer designing?

• A. There would be certainty because the genetic code is unique for each amino acid
• B. There would be uncertainty as the genetic code is degenerate and none of the amino acid is having a unique code
• C. There would be uncertainty as the genetic code is degenerate but some of the amino acids such as methionine are having a unique codon
• D. The amount of uncertainty or certainty is a matter of chance