Natural transformation can be relieved by the use of protoplasts, in the presence of osmotic buffer and polyethylene glycol. What are protoplasts?

• A. Protoplasts are the cells from which cell membrane has been removed
• B. Protoplasts are the cells from which cell wall has been removed
• C. Protoplasts are the cells from which vacuole has been removed
• D. Protoplasts are the cells from which golgi bodies are removed