MCQOPTIONS
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MCQOPTIONS
This section includes 20 Mcqs, each offering curated multiple-choice questions to sharpen your BITSAT knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The phenomenon of not allowing the recombinants to escape into the environment is termed as __________ |
| A. | blocking |
| B. | termination |
| C. | disablement |
| D. | inactivation |
| Answer» D. inactivation | |
| 2. |
Chloramphenicol amplification is carried out in various plasmids. Which of the statement is incorrect for it? |
| A. | It is used for increasing copy number in plasmids containing pMB1 origin |
| B. | Chloramphenicol promotes bacterial protein synthesis |
| C. | The protein synthesis is responsible for chromosomal DNA replication |
| D. | Chromosomal DNA replication is related to cell division |
| Answer» C. The protein synthesis is responsible for chromosomal DNA replication | |
| 3. |
If high copy number is there, the replication is called as ______ and if low copy number is there the replication is called as ______ |
| A. | stringent, relaxed |
| B. | relaxed, stringent |
| C. | relaxed, relaxed |
| D. | stringent, stringent |
| Answer» B. relaxed, stringent | |
| 4. |
If a plasmid is having two antibiotic resistant genes, say ampicillin resistant and chloramphenicol resistant. If the plasmid grows in ampicillin containing medium but not in chloramphenicol, what can be concluded? |
| A. | The insert is not present in any of the gene |
| B. | The insert is present in ampicillin gene but not in chloramphenicol gene |
| C. | The insert is present in choramphenicol gene but not in ampicillin gene |
| D. | The insert is present between both of the genes |
| Answer» D. The insert is present between both of the genes | |
| 5. |
Which of the statement is true for pBR322? |
| A. | It contains only an ampicillin resistance gene |
| B. | It contains both ampicillin resistant and tetracycline resistant gene |
| C. | The cloning site is present only in the ampicillin resistant gene |
| D. | It is a natural vector |
| Answer» C. The cloning site is present only in the ampicillin resistant gene | |
| 6. |
If a restriction site is 6 nucleotides long, what are the chances of finding it in a vector? |
| A. | Once every 46 base pairs |
| B. | Once every 64 base pairs |
| C. | Once every 24 base pairs |
| D. | Once every 16 base pairs |
| Answer» B. Once every 64 base pairs | |
| 7. |
Size of the vector is related to having a suitable single restriction site. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 8. |
Multiple cloning site (MCS) is defined as __________ |
| A. | site within the plasmid which contains a site for many restriction enzymes |
| B. | site within the plasmid which contains a site for many restriction enzymes and they are not present anywhere else in the plasmid |
| C. | as the site containing many sites for only one restriction enzyme |
| D. | cloning many inserts together |
| Answer» C. as the site containing many sites for only one restriction enzyme | |
| 9. |
It is required to distinguish between the cells that have taken up the vector and that have not. It is done by using __________ |
| A. | multiple cloning site |
| B. | origin of replication |
| C. | high copy number |
| D. | selectable marker |
| Answer» E. | |
| 10. |
What will be the consequence of not having an origin of replication (ori) in the vector? |
| A. | If an ori is absent, replication of vector would not take place |
| B. | As the cells divide after taking up the vector, both the daughter cells would be having the vector |
| C. | A colony of transformed colonies is observed |
| D. | The vector won’t be taken up by the cell |
| Answer» B. As the cells divide after taking up the vector, both the daughter cells would be having the vector | |
| 11. |
Which of the following cloning vector is used for insect and having a DNA genome? |
| A. | Cosmid |
| B. | P-element |
| C. | SV40 |
| D. | Baculovirus |
| Answer» E. | |
| 12. |
Name the first vector used for a mammalian cell? |
| A. | Lambda phage |
| B. | SV40 |
| C. | M13 |
| D. | EMBL3 |
| Answer» C. M13 | |
| 13. |
Name the vector system used for plant transformation? |
| A. | Agrobacterium |
| B. | Cosmids |
| C. | M13 phage |
| D. | Resistance plasmid |
| Answer» B. Cosmids | |
| 14. |
Which of the following is NOT an example of Lambda phages? |
| A. | EMBL3 |
| B. | M13 |
| C. | λgt10 |
| D. | λZAP |
| Answer» C. λgt10 | |
| 15. |
Which of the following is TRUE for YAC? |
| A. | Recognizes palindromic sequences |
| B. | Two fold axial symmetry |
| C. | Recognize specific site according to DNA sources |
| D. | Derived from DNA of the Yeast |
| Answer» E. | |
| 16. |
Which of the following is NOT the characteristics of the plasmid? |
| A. | Replicate independently in a host cell |
| B. | Selectable marker gene |
| C. | Contain single cloning site |
| D. | Small in size |
| Answer» D. Small in size | |
| 17. |
Cloning vector has the following properties, except one? |
| A. | Contain genetic marker for selection |
| B. | Ability to replicate |
| C. | Multiple restriction site for entry of DNA |
| D. | Contain nonessential DNA to optimize cloning |
| Answer» D. Contain nonessential DNA to optimize cloning | |
| 18. |
Which of the following is NOT a cloning vector? |
| A. | Bacterial plasmid |
| B. | Cosmids |
| C. | Bacteriophage |
| D. | E.coli |
| Answer» E. | |
| 19. |
Without origin of replication, DNA cannot be replicated in the cell. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 20. |
Circular DNA used for cloning is called___________ |
| A. | DNA polymerase |
| B. | RNA polymerase |
| C. | Phagosome |
| D. | Plasmid |
| Answer» E. | |