MCQOPTIONS
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MCQOPTIONS
This section includes 7 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The correct statement with respect to lacIq is __________ |
| A. | It is a mutant of lac repressor gene and decreases the level of repressor |
| B. | It doesn t control the lacZ gene |
| C. | It is important for the vectors to replicate in a low copy number |
| D. | It doesn t titrate out the repressor produced by wild type Lac-I gene |
| Answer» B. It doesn t control the lacZ gene | |
| 2. |
lacZ M15 represents __________ |
| A. | fully present lacZ gene |
| B. | it is not required in alpha complementation |
| C. | it represents the lacZ gene with M15 mutation |
| D. | it is partially deleted lacZ gene which is needed for alpha complementation |
| Answer» E. | |
| 3. |
endA mutation is often induced in host molecules. Choose the correct statement. |
| A. | It activates the gene for DNA-specific endonuclease |
| B. | It enhances the yield of plasmid DNA preparations only |
| C. | It enhances the quality of plasmid DNA preparations only |
| D. | It enhances both the quality and yield of plasmid DNA preparations |
| Answer» E. | |
| 4. |
Disablement is also done in plasmids. Choose the correct statement. |
| A. | The strains carrying out the recombinant plasmids should not escape outside |
| B. | Preferred strains are having mutations which allow their growth in wild |
| C. | Mutations confer prototrophy |
| D. | If the recombinants escape outside, there is no such harm |
| Answer» B. Preferred strains are having mutations which allow their growth in wild | |
| 5. |
Choose the incorrect statement with respect to dam and dcm genes? |
| A. | Their enzymes are responsible for methylation of DNA |
| B. | Dam protein methylates cytosine in GATC- |
| C. | Dcm methylates cytosine in-CCAGG- and CCTGG- sequences |
| D. | The extent of methylation also has effects on the efficiency with which the other restriction enzymes cleave the DNA in vitro |
| Answer» C. Dcm methylates cytosine in-CCAGG- and CCTGG- sequences | |
| 6. |
Endogenous DNA-degrading system is not essential in determining transformation efficiency. |
| A. | True |
| B. | False |
| Answer» C. | |
| 7. |
The cell in which the recombinant molecules are propagated is termed as __________ |
| A. | host |
| B. | vector |
| C. | plasmid |
| D. | carrier |
| Answer» B. vector | |