MCQOPTIONS
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MCQOPTIONS
This section includes 116 Mcqs, each offering curated multiple-choice questions to sharpen your Cell Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which observation was made by Avery, Macleod, and McCarty? |
| A. | DNA is a duplex molecule |
| B. | DNA can be taken up from medium |
| C. | DNA can denature at high temperatures |
| D. | DNA is more stable than RNA |
| Answer» C. DNA can denature at high temperatures | |
| 2. |
EcoR1 exhibits a two-fold rotational symmetry. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 3. |
The first recombinant DNA molecule was synthesized in the year ______________ |
| A. | 1962 |
| B. | 1972 |
| C. | 1982 |
| D. | 1992 |
| Answer» C. 1982 | |
| 4. |
Equilibrium centrifugation is also known as ___________________ centrifugation. |
| A. | isotonic |
| B. | isopycnic |
| C. | gradient |
| D. | sucrose |
| Answer» C. gradient | |
| 5. |
In purification steps of nucleic-acids, phenol is used for _______________________ |
| A. | deproteinization |
| B. | denaturation |
| C. | lowering pH |
| D. | lowering viscosity |
| Answer» B. denaturation | |
| 6. |
Which is the main ingredient in the sample preparation of mass spectrometry? |
| A. | papain |
| B. | pepsin |
| C. | vinculin |
| D. | trypsin |
| Answer» E. | |
| 7. |
Viral-mediated gene transfer is called _______________ |
| A. | conjugation |
| B. | transduction |
| C. | transformation |
| D. | transversion |
| Answer» C. transformation | |
| 8. |
Proteins with greater charge density move faster in the PAGE. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 9. |
Which enzyme is used to join together two different types of DNA molecules? |
| A. | ligase |
| B. | endonuclease |
| C. | exonuclease |
| D. | protease |
| Answer» B. endonuclease | |
| 10. |
Which of the following is used in PAGE to prevent the mixing of the sample with running buffer? |
| A. | ethanol |
| B. | methanol |
| C. | chloroform |
| D. | sucrose |
| Answer» E. | |
| 11. |
Proteins interacting with specific substances can be separated using ___________________ |
| A. | ion-exchange chromatography |
| B. | paper chromatography |
| C. | affinity chromatography |
| D. | gel-filtration chromatography |
| Answer» D. gel-filtration chromatography | |
| 12. |
In gel-filtration chromatography, the proteins ______________________ |
| A. | bind to the column |
| B. | diffuse through the column |
| C. | denature |
| D. | vaporize |
| Answer» C. denature | |
| 13. |
The procedure by which DNA gel is transferred to a membrane is termed as ___________________ |
| A. | blotting |
| B. | centifugation |
| C. | electrophoresis |
| D. | paper chromatography |
| Answer» B. centifugation | |
| 14. |
When was the technique of two-dimensional gel electrophoresis developed? |
| A. | 1955 |
| B. | 1965 |
| C. | 1975 |
| D. | 1985 |
| Answer» D. 1985 | |
| 15. |
Taq polymerase is a ________________________ polymerase. |
| A. | heat-stable |
| B. | buffering |
| C. | denaturant |
| D. | large |
| Answer» B. buffering | |
| 16. |
For a mass spectrometric analysis, the compounds are converted into ___________________ |
| A. | gaseous ions |
| B. | soluble form |
| C. | matrix |
| D. | antibody |
| Answer» B. soluble form | |
| 17. |
Who developed the chemical techniques to synthesize polynucleotides? |
| A. | Barbara McClintock |
| B. | James Watson |
| C. | Fredrick Sanger |
| D. | H. Gobind Khorana |
| Answer» E. | |
| 18. |
Protein’s solubility depends on the relative balance between protein-solvent and protein-protein interactions. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 19. |
DNA libraries are collection of ______________________ |
| A. | ribonucleic acid |
| B. | cloned DNA fragments |
| C. | bacteriophages |
| D. | viral particles |
| Answer» C. bacteriophages | |
| 20. |
Which of the following is used to lyse the nucleus and release the DNA? |
| A. | sodium dodecyl sulfate |
| B. | ammonium sulfate |
| C. | ferric phosphate |
| D. | fluorine |
| Answer» B. ammonium sulfate | |
| 21. |
Recombinant plasmids are added to a bacterial culture that has been pretreated with _________________ ions. |
| A. | iodine |
| B. | magnesium |
| C. | calcium |
| D. | ferric |
| Answer» D. ferric | |
| 22. |
In rate-zonal sedimentation, the nucleic acids are separated base on __________________ |
| A. | nucleotide length |
| B. | relative charge |
| C. | relative mass |
| D. | mass-to-charge ratio |
| Answer» B. relative charge | |
| 23. |
In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography? |
| A. | Western blotting |
| B. | Southern blotting |
| C. | Isopycnic centrifugation |
| D. | Spectrophotometry |
| Answer» C. Isopycnic centrifugation | |
| 24. |
Polyacrylamide gel electrophoresis uses ___________________ to separate proteins. |
| A. | pressure difference |
| B. | temperature difference |
| C. | electric field |
| D. | magnetic field |
| Answer» D. magnetic field | |
| 25. |
Which of the following can be used for selective precipitation of proteins? |
| A. | alcohol |
| B. | phenol |
| C. | ammonium sulfate |
| D. | sodium acetate |
| Answer» D. sodium acetate | |
| 26. |
Which was the first protein to have its structure determined using X-ray crystallography? |
| A. | keratin |
| B. | myoglobin |
| C. | immunoglobulin |
| D. | globulin |
| Answer» C. immunoglobulin | |
| 27. |
Which of the following is a commonly used label in blotting techniques? |
| A. | vimentin |
| B. | biotin |
| C. | avidin |
| D. | streptomycin |
| Answer» C. avidin | |
| 28. |
Which of the following uses non-compressible matrix and high pressure? |
| A. | HPLC |
| B. | GC-MS |
| C. | LC-MS |
| D. | MS-MS |
| Answer» B. GC-MS | |
| 29. |
Which of the following is radioactive? |
| A. | hydrogen sulfide |
| B. | vimentin |
| C. | tritium |
| D. | deuterium |
| Answer» D. deuterium | |
| 30. |
A beta particle is equivalent to an electron. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 31. |
At the critical drying point, there is no surface tension between ________________ |
| A. | gas and oil |
| B. | gas and liquid |
| C. | liquid and liquid |
| D. | oil and liquid |
| Answer» C. liquid and liquid | |
| 32. |
The biological materials have little intrinsic capability to ____________________ |
| A. | scatter electrons |
| B. | stain |
| C. | remain viable |
| D. | be captured |
| Answer» B. stain | |
| 33. |
Which of the following amino acid absorbs the light of 280 nm? |
| A. | tyrosine |
| B. | cysteine |
| C. | leucine |
| D. | valine |
| Answer» B. cysteine | |
| 34. |
Glutaraldehyde is a ________________ |
| A. | metal |
| B. | fixative |
| C. | non-metal |
| D. | atomic species |
| Answer» C. non-metal | |
| 35. |
PCR can generate large amounts of DNA. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 36. |
When the pH of a protein is lowered _________________________ |
| A. | temperature decreases |
| B. | negatively-charged groups neutralize |
| C. | positively-charged groups neutralize |
| D. | positively-charged groups decrease |
| Answer» C. positively-charged groups neutralize | |
| 37. |
Osmium is a ___________________ |
| A. | non metal |
| B. | heavy metal |
| C. | alloy |
| D. | light metal |
| Answer» C. alloy | |
| 38. |
Which of the following component of TEM focuses the beam of electrons on the sample? |
| A. | ocular lens |
| B. | condenser lens |
| C. | stage |
| D. | column |
| Answer» C. stage | |
| 39. |
In X-ray diffraction, the protein crystals are bombarded with ____________ |
| A. | UV rays |
| B. | X rays |
| C. | Gamma rays |
| D. | Infrared rays |
| Answer» C. Gamma rays | |
| 40. |
Bacteriophage Lambda is a major cloning vector. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 41. |
Electron cryomicroscopy is used mainly for _______________ |
| A. | soluble proteins |
| B. | non-soluble proteins |
| C. | carbohydrates |
| D. | lipids |
| Answer» C. carbohydrates | |
| 42. |
The half life of a radioisotope is _____________ |
| A. | half the time taken for complete decay |
| B. | half the time taken for half the decay |
| C. | time taken for complete decay |
| D. | time taken for half the decay |
| Answer» E. | |
| 43. |
Which of the following can be used to clone DNA sequence of size larger than 25 kb? |
| A. | Yeast artificial chromosome |
| B. | SV40 |
| C. | Plasmid |
| D. | Bacteriophage |
| Answer» B. SV40 | |
| 44. |
In mass-spectrometry, proteins are separated base on their _____________________ |
| A. | i-value |
| B. | c-value |
| C. | m/z ratio |
| D. | e/m ratio |
| Answer» D. e/m ratio | |
| 45. |
In the preparation of samples for SEM, the specimens are air-dried by a process of critical-point drying. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 46. |
Which enzyme is active at 72° in the polymerase chain reaction? |
| A. | isomerase |
| B. | exonuclease |
| C. | polymerase |
| D. | endonuclease |
| Answer» D. endonuclease | |
| 47. |
EDTA binds the ______________ ions. |
| A. | magnesium |
| B. | iron |
| C. | carbon |
| D. | calcium |
| Answer» E. | |
| 48. |
Synchrotrons generate _____________________ |
| A. | Peptides |
| B. | X rays |
| C. | Infrared rays |
| D. | Carcinogens |
| Answer» C. Infrared rays | |
| 49. |
HeLa cells are a cell line. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 50. |
Nucleic acid hybridization is used to identify ________________________ |
| A. | RNAs |
| B. | DNAs |
| C. | Complementary base sequences |
| D. | Proteins |
| Answer» D. Proteins | |