Explore topic-wise MCQs in Genetic Engineering.

This section includes 18 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

DpnI cuts ________ strands.

A. methylated
B. non-methylated
C. phosphorylated
D. non-phosphorylated
Answer» B. non-methylated
Discussion
2.

What is the function of ung gene?

A. It is responsible for deamination of cytosine
B. It is responsible for deamination of uracil
C. It is responsible for removal of uracil
D. It is responsible for removal of cytosine
Answer» D. It is responsible for removal of cytosine
Discussion
3.

In a phosphothiorate nucleotide, oxygen atom is replaced by with atom?

A. Magnesium
B. Calcium
C. Sodium
D. Sulphur
Answer» E.
Discussion
4.

Replication by first strand leads to the formation of mutated molecules and functional antibiotic resistant gene.

A. True
B. False
Answer» C.
Discussion
5.

Once second strand synthesis is carried out, it is introduced into the host. Host is having which mutation?

A. mutS mutation
B. mutD mutation
C. mutE mutation
D. mutG mutation
Answer» B. mutD mutation
Discussion
6.

If PCR is used to introduce random mutations rather than specific mutations, it is called as ___________

A. mutagenic PCR
B. error-prone PCR
C. random PCR
D. general PCR
Answer» C. random PCR
Discussion
7.

PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer.

A. True
B. False
Answer» B. False
Discussion
8.

REPLICATION_BY_FIRST_STRAND_LEADS_TO_THE_FORMATION_OF_MUTATED_MOLECULES_AND_FUNCTIONAL_ANTIBIOTIC_RESISTANT_GENE._IS_THE_GIVEN_STATEMENT_TRUE_OR_FALSE??$

A. True
B. False
Answer» C.
Discussion
9.

What is the function of ung gene?$

A. It is responsible for deamination of cytosine
B. It is responsible for deamination of uracil
C. It is responsible for removal of uracil
D. It is responsible for removal of cytosine
Answer» D. It is responsible for removal of cytosine
Discussion
10.

In_a_phosphothiorate_nucleotide,_oxygen_atom_is_replaced_by_with_atom?$

A. Magnesium
B. Calcium
C. Sodium
D. Sulphur
Answer» E.
Discussion
11.

DpnI_cuts__________strands.

A. methylated
B. non-methylated
C. phosphorylated
D. non-phosphorylated
Answer» B. non-methylated
Discussion
12.

Once second strand synthesis is carried out, it is introduced into host. Host is having which mutation?

A. mutS mutation
B. mutD mutation
C. mutE mutation
D. mutG mutation
Answer» B. mutD mutation
Discussion
13.

Choose the incorrect statement for the methodology of selection of molecules with mutant sequences.

A. A vector is used which is having antibiotic resistance gene
B. Apart from antibiotic resistance gene, a second antibiotic resistance gene is also present
C. There are two mutagenic primers which are used
D. The second strand synthesis is carried out by only one primer
Answer» E.
Discussion
14.

For the selection of the molecules having mutated sequence, which of the statement is true?

A. It is suitable for methods which are PCR based
B. It is suitable for methods which are not PCR based
C. It is suitable for both PCR and not PCR based
D. Selection of molecules with mutant sequence is not possible
Answer» C. It is suitable for both PCR and not PCR based
Discussion
15.

If PCR is used to introduce random mutations rather than specific mutations, it is called as:

A. mutagenic PCR
B. error-prone PCR
C. random PCR
D. general PCR
Answer» C. random PCR
Discussion
16.

Sometimes mutagenesis is carried out with the help of primers. Choose the correct statement with respect to it.

A. Double stranded circular molecule is used as a template
B. Mutation is introduced into one of flanking primers
C. Single stranded circular molecule is used as a template
D. After amplification, mutation is introduced into one strand
Answer» B. Mutation is introduced into one of flanking primers
Discussion
17.

PCR using the mutagenic primer and one of the flanking primers is used to carry out amplification and generates a product corresponding to the part of the gene. It is called as megaprimer. Is the given statement true or false?

A. True
B. False
Answer» B. False
Discussion
18.

A megaprimer method is a ____ stage approach and uses _____ oligonucleotide primers.

A. two, two
B. two, three
C. one, two
D. one, three
Answer» C. one, two
Discussion