MCQOPTIONS
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MCQOPTIONS
This section includes 12 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
If all the nucleotides are present with equal frequencies and at random, what are the chances of having a particular four nucleotide long motif? |
| A. | 1/256 |
| B. | 1/64 |
| C. | 1/16 |
| D. | 1/8 |
| Answer» B. 1/64 | |
| 2. |
The recognition sequence is at times palindromic in nature. Which of the following statements is correct with respect to it? |
| A. | The molecules which are cut by the same enzyme, anneal only if the sequence is palindromic in nature |
| B. | When the molecules are cleaved by the same enzyme and the recognition sequence is palindromic in nature, there is no effect on annealing |
| C. | There are increased chances of annealing if the recognition sequence is palindromic in nature |
| D. | The term ‘palindromic’ can be used whether the sequence is read from 5’ to 3’ or 3’ to 5’ |
| Answer» D. The term ‘palindromic’ can be used whether the sequence is read from 5’ to 3’ or 3’ to 5’ | |
| 3. |
The recognition sequence of Sau3A is 5’ |GATC 3’ and that for DpnI is 5’ GA|TC 3’. Which of the statements is true? |
| A. | The ends created by both the enzymes are compatible |
| B. | The ends created by both the enzymes are not-compatible |
| C. | The ends created by DpnI are single stranded |
| D. | The ends created by Sau3A are single stranded |
| Answer» C. The ends created by DpnI are single stranded | |
| 4. |
The recognition sequence for BamHI is 5’ G|GATCC 3’. The ‘|’ represents the cutting site. What can be inferred about the ends from it? |
| A. | The ends created are double stranded |
| B. | The single stranded end is 5’ in nature |
| C. | The single stranded end is 3’ in nature |
| D. | To decide about the nature of the ends more information is needed |
| Answer» C. The single stranded end is 3’ in nature | |
| 5. |
Blunt ends created by the restriction endonuclease can be joined. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 6. |
A sequence is having two ends, 5’ and 3’. Which of the following statements is correct regarding the nature of the ends? |
| A. | The 5’ end is having hydroxyl group |
| B. | The 5’ end is having phosphate group |
| C. | The 3’ end is having phosphate group |
| D. | Any group can be present at any end |
| Answer» C. The 3’ end is having phosphate group | |
| 7. |
After cleaving the sequence, the nature of the ends created by the type II endonuclease is __________ |
| A. | The ends created are always single stranded |
| B. | The ends created are always double stranded |
| C. | Either the ends are single stranded or they are double stranded |
| D. | One end is single stranded and one end is double stranded |
| Answer» D. One end is single stranded and one end is double stranded | |
| 8. |
Type II cuts the sequence in the following way __________ |
| A. | Within the recognition sequence |
| B. | At 100-1000 nucleotides away from the recognition sequence |
| C. | At 27-30 nucleotides away from the recognition sequence |
| D. | It cuts randomly |
| Answer» B. At 100-1000 nucleotides away from the recognition sequence | |
| 9. |
How many classes of restriction enzymes are there? |
| A. | 2 |
| B. | 1 |
| C. | 3 |
| D. | 4 |
| Answer» D. 4 | |
| 10. |
Even after replication, how the modified DNA remains protected? |
| A. | It remains protected because of conservative mode of replication |
| B. | It remains protected because of semi-conservative mode of replication |
| C. | The mode of replication has no role to play in the protection |
| D. | It is again modified after replication |
| Answer» C. The mode of replication has no role to play in the protection | |
| 11. |
The restriction endonuclease is having a defence mechanism in the bacterial system against foreign DNA such as viruses. But how it is able to protect its own DNA? |
| A. | By methylation of bacterial DNA by restriction enzyme |
| B. | By methylation of foreign DNA by restriction enzyme |
| C. | By phosphorylation of bacterial DNA by restriction enzyme |
| D. | By phosphorylation of foreign DNA by restriction enzyme |
| Answer» B. By methylation of foreign DNA by restriction enzyme | |
| 12. |
The term ‘endonuclease’ refers to cutting the DNA sequence from ______________ |
| A. | only within the polynucleotide chain, not at the ends |
| B. | the ends of the chain |
| C. | anywhere in the chain |
| D. | exactly in the middle of the chain |
| Answer» B. the ends of the chain | |