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This section includes 8 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
1. |
Purification of DNA by using silica derivatized groups by DEAE is termed as ____________ |
A. | ion exchange resin based method. |
B. | silica based purification |
C. | atom based resin exchange method |
D. | packed bed purification |
Answer» B. silica based purification | |
2. |
Which of the following components bind to the solid column made of silica, under high salt concentration? |
A. | Proteins |
B. | Polysaccharides |
C. | Both proteins and polysaccharides |
D. | Plasmid DNA |
Answer» E. | |
3. |
Adsorption onto a solid phase support followed by elution is used as an alternative for separation of which component? |
A. | chromosomal DNA |
B. | plasmid DNA |
C. | RNA alone |
D. | other impurities |
Answer» C. RNA alone | |
4. |
Treatment with exonuclease leads to removal of ____________ |
A. | remnants of chromosomal DNA |
B. | RNase |
C. | plasmid DNA which is circularized |
D. | proteins |
Answer» B. RNase | |
5. |
Nucleic acid precipitated constitutes of ____________ |
A. | plasmid DNA |
B. | plasmid DNA, along with RNA and chromosomal DNA |
C. | rna alone |
D. | chromosomal DNA only |
Answer» C. rna alone | |
6. |
The nucleic acid remaining in the solution can be precipitated by addition of sodium or ammonium acetate and ethanol. |
A. | False |
B. | True |
Answer» C. | |
7. |
Chromosomal or genomic DNA is separated by ____________ |
A. | Sedimentation |
B. | Dissolution in water |
C. | Centrifugation |
D. | Distillation |
Answer» D. Distillation | |
8. |
Isolation of genomic DNA follows the same principles as that of obtaining plasmid from E. coli. Which of the following is not included in it? |
A. | Cell lysis |
B. | Removal of proteins |
C. | Removal of chromosomal DNA |
D. | Dissolving plasmid in water |
Answer» E. | |