MCQOPTIONS
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MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Cell Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Polyacrylamide gel electrophoresis uses ___________________ to separate proteins. |
| A. | pressure difference |
| B. | temperature difference |
| C. | electric field |
| D. | magnetic field |
| Answer» D. magnetic field | |
| 2. |
The yeast two-hybrid system is used for studying ________________________ |
| A. | protein-protein interactions |
| B. | pressure changes |
| C. | molecular size |
| D. | differentiation pattern |
| Answer» B. pressure changes | |
| 3. |
Proteins interacting with specific substances can be separated using ___________________ |
| A. | ion-exchange chromatography |
| B. | paper chromatography |
| C. | affinity chromatography |
| D. | gel-filtration chromatography |
| Answer» D. gel-filtration chromatography | |
| 4. |
In gel-filtration chromatography, the proteins ______________________ |
| A. | bind to the column |
| B. | diffuse through the column |
| C. | denature |
| D. | vaporize |
| Answer» C. denature | |
| 5. |
Gel-filtration chromatography separates proteins based on their ___________________ |
| A. | pH |
| B. | temperature |
| C. | morphology |
| D. | effective size |
| Answer» E. | |
| 6. |
In ion-exchange chromatography, proteins bound to the resin can be displaced by increasing the _____________________ |
| A. | strength of ionic buffer |
| B. | size of sample |
| C. | column volume |
| D. | column width |
| Answer» B. size of sample | |
| 7. |
Which of the following is used as an ion-exchanger resin? |
| A. | ethanol |
| B. | cellulose |
| C. | starch |
| D. | collagen |
| Answer» C. starch | |
| 8. |
A protein is neutral at the isoelectric point. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 9. |
When the pH of a protein is lowered _________________________ |
| A. | temperature decreases |
| B. | negatively-charged groups neutralize |
| C. | positively-charged groups neutralize |
| D. | positively-charged groups decrease |
| Answer» C. positively-charged groups neutralize | |
| 10. |
Which of the following uses non-compressible matrix and high pressure? |
| A. | HPLC |
| B. | GC-MS |
| C. | LC-MS |
| D. | MS-MS |
| Answer» B. GC-MS | |
| 11. |
In the liquid column chromatography, there are two phases namely ___________________ and ____________________ |
| A. | mobile, immobile |
| B. | liquid, gel |
| C. | viscous, non-viscous |
| D. | flammable, inflammable |
| Answer» B. liquid, gel | |
| 12. |
Which of the following can be used for selective precipitation of proteins? |
| A. | alcohol |
| B. | phenol |
| C. | ammonium sulfate |
| D. | sodium acetate |
| Answer» D. sodium acetate | |
| 13. |
Protein’s solubility depends on the relative balance between protein-solvent and protein-protein interactions. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 14. |
Total nitrogen measurement can be used to measure ____________ |
| A. | pH drift |
| B. | total protein |
| C. | specific enzyme |
| D. | viscosity |
| Answer» C. specific enzyme | |
| 15. |
Purification of a protein can be measured as an increase in _____________ |
| A. | temperature |
| B. | pH value |
| C. | specific activity |
| D. | polarity |
| Answer» D. polarity | |