MCQOPTIONS
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MCQOPTIONS
This section includes 6 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Template independent polymerases are the enzymes which add nucleotide bases without a template. Which of the following statements is correct with respect to these? |
| A. | They only add a single nucleotide |
| B. | They only add a string of nucleotides and not a single nucleotide |
| C. | Terminal transferase adds a series of nucleotides at the 3 end |
| D. | Taq polymerase adds a single nucleotide at the 5 end of the PCR product |
| Answer» D. Taq polymerase adds a single nucleotide at the 5 end of the PCR product | |
| 2. |
Which polymerase can be used in conjunction with appropriate phage promoters in order to have high levels of specific transcription? |
| A. | RNA dependent DNA polymerase |
| B. | DNA dependent RNA polymerase |
| C. | RNA dependent RNA polymerase |
| D. | DNA dependent DNA polymerase |
| Answer» C. RNA dependent RNA polymerase | |
| 3. |
Why reverse transcriptase enzymes are having comparatively high error rates than other polymerases? |
| A. | Because they are not having 3 -5 proofreading exonucleolytic activity |
| B. | Because they are not having 5 -3 proofreading exonucleolytic activity |
| C. | Because they are having slow rates of exonucleolytic activity |
| D. | It is difficult to synthesize DNA from RNA |
| Answer» B. Because they are not having 5 -3 proofreading exonucleolytic activity | |
| 4. |
Which of the following enzyme is said as reverse transcriptase? |
| A. | DNA dependent DNA polymerase |
| B. | RNA dependent RNA polymerase |
| C. | RNA dependent DNA polymerase |
| D. | DNA dependent RNA polymerase |
| Answer» D. DNA dependent RNA polymerase | |
| 5. |
Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________ |
| A. | polishing and end filling respectively |
| B. | end filling and polishing respectively |
| C. | polishing in both the cases |
| D. | end filling in both the cases |
| Answer» B. end filling and polishing respectively | |
| 6. |
Polymerase can be defined as ______________ |
| A. | an enzyme used to synthesize a new DNA or RNA strand on the basis of pre-existing strand or at times without a pre-existing strand |
| B. | an enzyme used for removal of nucleotides from the DNA or RNA strand |
| C. | an enzyme which can synthesize only a new DNA strand, not an RNA strand |
| D. | an enzyme which can synthesize either a new DNA or an RNA strand but only when a strand is there |
| Answer» B. an enzyme used for removal of nucleotides from the DNA or RNA strand | |