MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 16 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Template independent polymerases are the enzymes which add nucleotide bases without a template. Which of the following statements is correct with respect to these? |
| A. | They only add a single nucleotide |
| B. | They only add a string of nucleotides and not a single nucleotide |
| C. | Terminal transferase adds a series of nucleotides at the 3’ end |
| D. | Taq polymerase adds a single nucleotide at the 5’ end of the PCR product |
| Answer» D. Taq polymerase adds a single nucleotide at the 5’ end of the PCR product | |
| 2. |
Which polymerase can be used in conjunction with appropriate phage promoters in order to have high levels of specific transcription? |
| A. | RNA dependent DNA polymerase |
| B. | DNA dependent RNA polymerase |
| C. | RNA dependent RNA polymerase |
| D. | DNA dependent DNA polymerase |
| Answer» C. RNA dependent RNA polymerase | |
| 3. |
Why reverse transcriptase enzymes are having comparatively high error rates than other polymerases? |
| A. | Because they are not having 3’-5’ proofreading exonucleolytic activity |
| B. | Because they are not having 5’-3’ proofreading exonucleolytic activity |
| C. | Because they are having slow rates of exonucleolytic activity |
| D. | It is difficult to synthesize DNA from RNA |
| Answer» B. Because they are not having 5’-3’ proofreading exonucleolytic activity | |
| 4. |
Which of the following enzyme is said as reverse transcriptase? |
| A. | DNA dependent DNA polymerase |
| B. | RNA dependent RNA polymerase |
| C. | RNA dependent DNA polymerase |
| D. | DNA dependent RNA polymerase |
| Answer» D. DNA dependent RNA polymerase | |
| 5. |
Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as _____________ |
| A. | polishing and end filling respectively |
| B. | end filling and polishing respectively |
| C. | polishing in both the cases |
| D. | end filling in both the cases |
| Answer» B. end filling and polishing respectively | |
| 6. |
Polymerase can be defined as ______________ |
| A. | an enzyme used to synthesize a new DNA or RNA strand on the basis of pre-existing strand or at times without a pre-existing strand |
| B. | an enzyme used for removal of nucleotides from the DNA or RNA strand |
| C. | an enzyme which can synthesize only a new DNA strand, not an RNA strand |
| D. | an enzyme which can synthesize either a new DNA or an RNA strand but only when a strand is there |
| Answer» B. an enzyme used for removal of nucleotides from the DNA or RNA strand | |
| 7. |
WHICH_POLYMERASE_CAN_BE_USED_IN_CONJUNCTION_WITH_APPROPRIATE_PHAGE_PROMOTERS_IN_ORDER_TO_HAVE_HIGH_LEVELS_OF_SPECIFIC_TRANSCRIPTION??$ |
| A. | RNA dependent DNA polymerase |
| B. | DNA dependent RNA polymerase |
| C. | RNA dependent RNA polymerase |
| D. | DNA dependent DNA polymerase |
| Answer» C. RNA dependent RNA polymerase | |
| 8. |
Template independent polymerases are the enzymes which add nucleotide bases without a template. Which of the following statements is correct with respect to these?$ |
| A. | They only add a single nucleotide |
| B. | They only add a string of nucleotides and not a single nucleotide |
| C. | Terminal transferase adds a series of nucleotides at the 3’ end |
| D. | Taq polymerase adds a single nucleotide at the 5’ end of the PCR product |
| Answer» D. Taq polymerase adds a single nucleotide at the 5‚Äö√Ñ√∂‚àö√ë‚àö¬• end of the PCR product | |
| 9. |
Why reverse transciptase enzymes are having comparatively high error rates than other polymerases? |
| A. | Because they are not having 3’-5’ proofreading exonucleolytic activity |
| B. | Because they are not having 5’-3’ proofreading exonucleolytic activity |
| C. | Because they are having slow rates of exonucleolytic activity |
| D. | It is difficult to synthesize DNA from RNA |
| Answer» B. Because they are not having 5‚Äö√Ñ√∂‚àö√ë‚àö¬•-3‚Äö√Ñ√∂‚àö√ë‚àö¬• proofreading exonucleolytic activity | |
| 10. |
Which of the following enzyme is said as reverse transciptase? |
| A. | DNA dependent DNA polymerase |
| B. | RNA dependent RNA polymerase |
| C. | RNA dependent DNA polymerase |
| D. | DNA dependent RNA polymerase |
| Answer» D. DNA dependent RNA polymerase | |
| 11. |
Thermostable DNA polymerases are very important in PCR. How are they obtained? |
| A. | They are obtained by heating the bacteria manually over high temperatures |
| B. | They are isolated from extremely stable thermophilic bacteria which are often found growing in oceanic vents |
| C. | They are found everywhere in the nature |
| D. | They are obtained by genetically modifying the E. coli bacteria with thermal stability property |
| Answer» C. They are found everywhere in the nature | |
| 12. |
Removal of single stranded portions produced due to exonucleolytic activity and due to polymerase activity are termed as: |
| A. | polishing and end filling respectively |
| B. | end filling and polishing respectively |
| C. | polishing in both the cases |
| D. | end filling in both the cases |
| Answer» B. end filling and polishing respectively | |
| 13. |
The E. coli DNA polymerase enzyme gives different domains with different activities on cleaving with protease subtilisin. Which of the following statements is correct with respect to the fragments generated? |
| A. | The smaller fragment is having C terminal and the larger fragment is having N terminal |
| B. | The smaller fragment is named as Klenlow fragment and the intact molecule is called as Kornberg fragment |
| C. | The smaller fragment is having 5’-3’ exonuclease activity whereas the larger fragment is having 5’-3’ polymerase and 3’-5’ exonuclease activity |
| D. | Both the fragments are having 5’-3’ polymerase and 3’-5’ exonuclease activity |
| Answer» D. Both the fragments are having 5‚Äö√Ñ√∂‚àö√ë‚àö¬•-3‚Äö√Ñ√∂‚àö√ë‚àö¬• polymerase and 3‚Äö√Ñ√∂‚àö√ë‚àö¬•-5‚Äö√Ñ√∂‚àö√ë‚àö¬• exonuclease activity | |
| 14. |
Which of the following activity is not possible in the case of DNA polymerase I? |
| A. | 3’-5’ exonuclease |
| B. | 5’-3’ exonuclease |
| C. | 5’-3’ DNA synthesis |
| D. | 3’-5’ DNA synthesis |
| Answer» E. | |
| 15. |
Polymerase can be defined as: |
| A. | an enzyme used to synthesize a new DNA or RNA strand on the basis of pre-existing strand or at times without a pre-existing strand |
| B. | an enzyme used for removal of nucleotides from the DNA or RNA strand |
| C. | an enzyme which can synthesize only a new DNA strand, not a RNA strand |
| D. | an enzyme which can synthesize either a new DNA or a RNA strand but only when a strand is there |
| Answer» B. an enzyme used for removal of nucleotides from the DNA or RNA strand | |
| 16. |
Basic classification of polymerases includes how many types? |
| A. | 2 |
| B. | 3 |
| C. | 4 |
| D. | 5 |
| Answer» E. | |