MCQOPTIONS
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MCQOPTIONS
This section includes 163 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 101. |
Expression of proteins using nuclear polyhedrosis viruses is advantageous because it gives _______ protein yields and post-translational modifications are _________ |
| A. | high, not possible |
| B. | high, possible |
| C. | low, possible |
| D. | low, not possible |
| Answer» C. low, possible | |
| 102. |
Cells that have lost SUP4 gene acquire _____ pigment and are visibly distinguishable from others. |
| A. | red |
| B. | blue |
| C. | green |
| D. | pink |
| Answer» B. blue | |
| 103. |
Cells mutant in _____ gene can be selected by using 5-fluoro orotic acid, which is turned into ____ products by wild type protein. |
| A. | SUP4, toxic |
| B. | SUP4, non-toxic |
| C. | URA3, toxic |
| D. | URA3, non-toxic |
| Answer» D. URA3, non-toxic | |
| 104. |
Phosphoribosyl amino-imidazole carboxylase is a component of _______ biosynthesis pathway. |
| A. | pyrimidine |
| B. | purine |
| C. | both purine and pyrimidine |
| D. | only adenine |
| Answer» C. both purine and pyrimidine | |
| 105. |
Yeast centromeric plasmid (YCp50) contains ampicillin and tetracycline resistant gene. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 106. |
Loss of SUP4 abolishes canavanine uptake, it is arginine analogue. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 107. |
The transfer of plasmid from one bacterial cell to another when cargo and conjugal plasmids are used, it is usually is carried out by _____ mating. |
| A. | diparental |
| B. | uniparental |
| C. | triparental |
| D. | multiparental |
| Answer» D. multiparental | |
| 108. |
Transfer of plasmids from one cell to another can also be done conjugation. The plasmid to be transferred known as ______ is often unable to do so by itself and thus it relies on other plasmids known as ______ |
| A. | cargo, conjugal |
| B. | conjugal, cargo |
| C. | cargo, helper |
| D. | conjugal, helper |
| Answer» B. conjugal, cargo | |
| 109. |
Members of genera Haematococcus are used for production of ____________ |
| A. | xanthenoid |
| B. | carotenoid |
| C. | chlorophyll |
| D. | carotenoid and xanthenoid both |
| Answer» C. chlorophyll | |
| 110. |
For Chlorella, which method is used for DNA uptake? |
| A. | Electroporation |
| B. | Direct uptake of naked DNA |
| C. | Conjugation |
| D. | Chemically induced uptake by protoplast |
| Answer» E. | |
| 111. |
Genetic manipulation of Volvox carteri is carried out using nitrogen reducatse or antibiotic resistance. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't Say |
| Answer» B. False | |
| 112. |
cw15 is a mutant strain, it ________ and thus called as ________ |
| A. | lacks cell wall, natural protoplast |
| B. | lacks cell wall, artificial protoplast |
| C. | has cell wall, artificial protoplast |
| D. | has cell wall, natural protoplast |
| Answer» B. lacks cell wall, artificial protoplast | |
| 113. |
Homologue of yeast ARG4 in Chlamydomonas is __________ |
| A. | ARG1 |
| B. | ARG2 |
| C. | ARG5 |
| D. | ARG7 |
| Answer» E. | |
| 114. |
T protein is required for replication. It is a product of ____________ |
| A. | delayed early transcription |
| B. | late transcription |
| C. | early transcription |
| D. | both early and late replication |
| Answer» D. both early and late replication | |
| 115. |
It is beneficial to use retrovirus for genetic manipulation of cells in the culture. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 116. |
It is necessary to analyze the genome of transgenic cells to confirm that the site of integration is correct. This is carried out by southern blotting. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 117. |
For introduction of exogenous DNA into S.cerevisiae ______ is added along with polyethylene glycol and heat shock treatment. |
| A. | calcium chloride |
| B. | sodium acetate |
| C. | lithium acetate |
| D. | magnesium chloride |
| Answer» D. magnesium chloride | |
| 118. |
Hygromycin is used as a selectable marker in mammalian cultured cells. It is used for ____________ |
| A. | initiating protein synthesis |
| B. | inhibiting protein synthesis |
| C. | initiating DNA binding process |
| D. | inhibiting DNA binding process |
| Answer» C. initiating DNA binding process | |
| 119. |
One of the commonly used markers is the URA3 gene. It encodes for ______ biosynthesis. |
| A. | adenine |
| B. | uracil |
| C. | guanine |
| D. | cytosine |
| Answer» C. guanine | |
| 120. |
If a gene is inactivated by gene targeting then it is called as ____________ |
| A. | knock-in gene |
| B. | knock-out gene |
| C. | gene disruption |
| D. | insertional inactivation |
| Answer» C. gene disruption | |
| 121. |
atpB encodes _______ subunit of ATP synthase, an enzyme used for generation of ______ |
| A. | beta, ADP |
| B. | alpha, ATP |
| C. | beta, ATP |
| D. | alpha, ADP |
| Answer» D. alpha, ADP | |
| 122. |
Transient gene silencing can be carried out by microinjecting ______ |
| A. | single stranded RNA |
| B. | double stranded DNA |
| C. | double stranded RNA |
| D. | either double stranded DNA or RNA |
| Answer» D. either double stranded DNA or RNA | |
| 123. |
Embryonic stem cells (ES) are isolated and are injected again into the blastocoel of a developing embryo. The embryo which develops is entirely made up of these cells only. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» C. May be True or False | |
| 124. |
nmt1+ gene is used for _____ biosynthesis. |
| A. | uracil |
| B. | adenine |
| C. | cytosine |
| D. | thiamine |
| Answer» E. | |
| 125. |
Transcription of GAL1 and GAL10 is suppressed in the presence of _______ |
| A. | glucose |
| B. | galactose |
| C. | maltose |
| D. | fructose |
| Answer» B. galactose | |
| 126. |
COX3 gene is a selectable marker. Choose the correct statement with respect to it. |
| A. | It confers the ability to grow by anaerobic respiration |
| B. | It confers the ability to grow by aerobic respiration |
| C. | It confers the ability to grow in absence of uracil |
| D. | It confers the ability to grow in lithium acetate medium |
| Answer» C. It confers the ability to grow in absence of uracil | |
| 127. |
Agrobacterium tumefaciens form ______ plasmids and Agrobacterium rhizogenes form _______ plasmids. |
| A. | root inducing, tumour inducing |
| B. | tumour inducing, root inducing |
| C. | tumour inducing, shoot inducing |
| D. | non-tumour inducing, shoot inducing |
| Answer» C. tumour inducing, shoot inducing | |
| 128. |
The replacement of one plasmid by another by introduction of the second plasmid into strain containing the first, followed by removal of the first by counterselection is known as __________ |
| A. | shuffle vector |
| B. | plasmid shuffle |
| C. | counterselection plasmid |
| D. | combination plasmid |
| Answer» C. counterselection plasmid | |
| 129. |
rosy gene is used as a selectable marker for transformation in Drosophila, it produces an enzyme required for the synthesis of ____________ |
| A. | wing pigment |
| B. | eye pigment |
| C. | both eye and wing pigment |
| D. | thorax pigment |
| Answer» C. both eye and wing pigment | |
| 130. |
What is the function of glyphosate? |
| A. | It is a fungicide |
| B. | It is an herbicide |
| C. | It is an enzyme used in place of glucose as a carbon source |
| D. | It is used for adding phosphate groups |
| Answer» C. It is an enzyme used in place of glucose as a carbon source | |
| 131. |
Choose the incorrect statement for shuttle vectors. |
| A. | These are vector hybrids constructed from E.coli and other plasmids |
| B. | They are having a varied use |
| C. | They can replicate and selected in both the species |
| D. | They are the plasmids which are having naturally broad host range |
| Answer» E. | |
| 132. |
If a plasmid can’t be transferred from one cell to another, then it is called as ___________ |
| A. | non-transmissible |
| B. | non-mobilizable |
| C. | untransferrable |
| D. | immobilized |
| Answer» C. untransferrable | |
| 133. |
If plasmids direct their own transfer from one bacterium cell to another, then they are called as ___________ |
| A. | self-transmissible |
| B. | auto-transmissible |
| C. | autonomously replicating |
| D. | auto-transfer |
| Answer» B. auto-transmissible | |
| 134. |
E.coli is a gram negative bacterium. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 135. |
An organism containing a gene which doesn’t belong to it and is derived from somewhere else then the organism is said to be ___________ |
| A. | transformed |
| B. | transgenic |
| C. | mutant |
| D. | modified |
| Answer» C. mutant | |
| 136. |
Technique of inserting Deoxyribonucleic Acid (DNA) into plants is known as |
| A. | bio-injection |
| B. | bio-fission |
| C. | bio-genetic |
| D. | bio-diffusion |
| Answer» C. bio-genetic | |
| 137. |
Technique of inserting DNA (Deoxyribonucleic Acid) into animal cells is known as |
| A. | micro injection |
| B. | macro injection |
| C. | fusion injection |
| D. | genome injection |
| Answer» B. macro injection | |
| 138. |
Traditional breeding methods are |
| A. | selective breeding |
| B. | cell fusion |
| C. | mutation breeding |
| D. | all of above |
| Answer» E. | |
| 139. |
Process of replacing defective genes with effective genes is known as |
| A. | transgenic fusion |
| B. | transgenic budding |
| C. | transgenic injection |
| D. | gene therapy |
| Answer» E. | |
| 140. |
Element which allows easy visualization of genetic modification products is known as |
| A. | green fluorescent protein |
| B. | blue fluorescent protein |
| C. | white fluorescent protein |
| D. | red fluorescent protein |
| Answer» B. blue fluorescent protein | |
| 141. |
For carrying out gene manipulation, the use of cultured cells is _____ transgenic organisms. |
| A. | less reliable |
| B. | more reliable |
| C. | may be less or more reliable |
| D. | is same reliable as |
| Answer» B. more reliable | |
| 142. |
The gene targeting approach produces individuals which are ______ for inactivation of the gene. |
| A. | homozygous |
| B. | heterozygous |
| C. | either only homozygous or only heterozygous |
| D. | both heterozygous and homozygous |
| Answer» C. either only homozygous or only heterozygous | |
| 143. |
If controlled inactivation of a gene is carried out and some of the consequences when inactivation of a target gene is deleterious are avoided. It is referred as ____________ |
| A. | specialized gene targeting |
| B. | controlled gene targeting |
| C. | conditional gene targeting |
| D. | specific gene targeting |
| Answer» D. specific gene targeting | |
| 144. |
OEE1 gene encodes a component of _________ |
| A. | photosystem I |
| B. | photosystem II |
| C. | both photosystem I and II |
| D. | which neither belongs to photosystem I nor II |
| Answer» C. both photosystem I and II | |
| 145. |
Transformation of Chlamydomonas is most efficient in which method? |
| A. | Lithium acetate method |
| B. | Electroporation |
| C. | Protoplast based method |
| D. | Both electroporation and protoplast based method |
| Answer» D. Both electroporation and protoplast based method | |
| 146. |
If a host other than E.coli is to be used, what property of DNA to be inserted is disadvantageous? |
| A. | Circular DNA |
| B. | Linear DNA |
| C. | Replicating DNA |
| D. | Non-replicating DNA |
| Answer» D. Non-replicating DNA | |
| 147. |
If genetic material of another species is added into the host then organism resulting from this is classified as |
| A. | clone genes |
| B. | transgenic |
| C. | cisgenic |
| D. | cross genes |
| Answer» C. cisgenic | |
| 148. |
If genetic material from same species is used or species that can naturally breed with the host then the organism generated is |
| A. | clone genes |
| B. | transgenic |
| C. | cisgenic |
| D. | cross genes |
| Answer» D. cross genes | |
| 149. |
The technique which uses homologous recombination to delete, add or change a gene is known as |
| A. | gene bundling |
| B. | gene linking |
| C. | gene targeting |
| D. | gene replication |
| Answer» D. gene replication | |
| 150. |
Transformation method of plants and animals in which plants and animals are given shocks is known as |
| A. | microinjection |
| B. | genome breeding |
| C. | electroporation |
| D. | genome engineering |
| Answer» D. genome engineering | |