MCQOPTIONS

Explore topic-wise MCQs in Genetic Engineering.

This section includes 17 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.	What are the effects of increasing concentration of reaction components?
A.	It increases chances of ligation in both intramolecular and intermolecular reactions
B.	It increases chances of ligation only in intermolecular and no effect on intramolecular
C.	It decreases chances of ligation in intramolecular and increases in that of intermolecular
D.	It decreases chances of ligation in both types of reaction
Answer» C. It decreases chances of ligation in intramolecular and increases in that of intermolecular

2.	What is the kinetics of the intramolecular and intermolecular ligation reactions?
A.	Second order kinetics for intramolecular and first order for intermolecular
B.	First order kinetics for intramolecular and second order for intermolecular
C.	Both are first order
D.	Both are second order
Answer» C. Both are first order

3.	Ligation reaction can be both intramolecular and intermolecular in nature.
A.	True
B.	False
Answer» B. False

4.	In the case of blunt-end ligation, blunt ends can be generated by ___________
A.	simply the action of restriction endonuclease which gives straight ends
B.	the polishing of staggered ends
C.	both the action of restriction endonuclease which gives straight ends and polishing of staggered ends
D.	by the action of restriction endonuclease which gives staggered ends
Answer» D. by the action of restriction endonuclease which gives staggered ends

5.	If only one bond is broken in the sugar-phosphate backbone, it is called as ___________
A.	gap
B.	nick
C.	break
D.	leakage
Answer» C. break

6.	Ligation is defined as ______________
A.	Alignment of only double stranded DNA molecules at the ends and the formation of phosphodiester bonds between both the strands
B.	Alignment of either of the double or single stranded DNA molecules and formation of glycosidic bonds between both the strands
C.	Alignment of either of the double or single stranded DNA molecules and the formation of phosphodiester bonds. The bond can be between either one or both the strands
D.	Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands
Answer» D. Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands

7.	WHAT_IS_THE_KINETICS_OF_THE_INTRAMOLECULAR_AND_INTERMOLECULAR_LIGATION_REACTIONS??$
A.	Second order kinetics for intramolecular and first order for intermolecular
B.	First order kinetics for intramolecular and second order for intermolecular
C.	Both are first order
D.	Both are second order
Answer» C. Both are first order

8.	What are the effects of increasing concentration of reaction components?$
A.	It increases chances of ligation in both intramolecular and intermolecular reactions
B.	It increases chances of ligation only in intermolecular and no effect on intramolecular
C.	It decreases chances of ligation in intramolecular and increase in that of intermolecular
D.	It decreases chances of ligation in both types of reaction
Answer» C. It decreases chances of ligation in intramolecular and increase in that of intermolecular

9.	If a ligation reaction is being carried out and recircularization is observed, which type of reaction is being carried out?
A.	Intramolecular
B.	Intermolecular
C.	Both observe recircularization equally
D.	Recircurlization is not possible in any of the case
Answer» B. Intermolecular

10.	Ligation reaction can be both intramolecular and intermolecular in nature. True or false?
A.	True
B.	False
Answer» B. False

11.	Why sticky ended ligations are carried out at temperatures lower than room temperature?
A.	It is so because the vibrational and kinetic energy of the molecules at room temperature is lower than that of the energy required to break the bonds holding the ends
B.	The energy required to break the bonds holding the ends is very less than that of the kinetic and vibrational energy at room temperature
C.	The enzyme carrying out ligation is unstable at low temperature
D.	The sticky ends created, don‚Äö√Ñ√∂‚àö√ë‚àö¬•t just religate at low temperature
Answer» B. The energy required to break the bonds holding the ends is very less than that of the kinetic and vibrational energy at room temperature

12.	The sticky ends are held together by which type of bonds?
A.	Hydrogen bond
B.	Covalent bond
C.	Ionic bond
D.	Van-der-waal forces
Answer» B. Covalent bond

13.	The ligation reaction is more efficient in which case?
A.	Blunt end ligation
B.	Sticky end ligation
C.	Both have same efficiency
D.	Depends on the reaction conditions
Answer» C. Both have same efficiency

14.	In the case of blunt-end ligation, blunt ends can be generated by:
A.	simply the action of restriction endonuclease which gives straight ends
B.	the polishing of staggered ends
C.	both the action of restriction endonuclease which give straight ends and polishing of staggered ends
D.	by the action of restriction endonuclease which gives staggered ends
Answer» D. by the action of restriction endonuclease which gives staggered ends

15.	How many categories of ligation reaction are there on the basis of ends created?
A.	1
B.	2
C.	3
D.	4
Answer» C. 3

16.	If only one bond is broken in the sugar-phosphate backbone, it is called as:
A.	gap
B.	nick
C.	break
D.	leakage
Answer» C. break

17.	Ligation is defined as:
A.	Alignment of only double stranded DNA molecules at the ends and the formation of phosphodiester bonds between both the strands
B.	Alignment of either of the double or single stranded DNA molecules and formation of glycosidic bonds between both the strands
C.	Alignment of either of the double or single stranded DNA molecules and then formation of phosphodiester bonds. The bond can be between either one or both the strands
D.	Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands
Answer» D. Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands