Explore topic-wise MCQs in Genetic Engineering.

This section includes 17 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

Cis-acting REP 3 sequence is present in Yeast episomal plasmids. Choose the statement which holds true for it?

A. It is present along with chromosomal centromeric sequence
B. It is the site of action of proteins that help in partitioning
C. It gives less stability to that of YCs
D. The copy number is low than that of YCs
Answer» C. It gives less stability to that of YCs
Discussion
2.

Yeast episomal plasmids have the following feature?

A. They contain ARS and CEL both
B. They contain ARS but not CEL
C. They contain CEL but not ARS
D. CEL is necessarily present but ARS may or may not be present
Answer» C. They contain CEL but not ARS
Discussion
3.

CEN4 in YCp50 is an example of _______

A. tetracycline resistant gene
B. ampicillin resistant gene
C. chromosomal centromeric sequence
D. autonomously replicating sequence
Answer» D. autonomously replicating sequence
Discussion
4.

Yeast centromeric plasmid (YCp50) contains ampicillin and tetracycline resistant gene.

A. True
B. False
Answer» B. False
Discussion
5.

Loss of SUP4 abolishes canavanine uptake, it is arginine analogue.

A. True
B. False
Answer» B. False
Discussion
6.

One of the commonly used markers is the URA3 gene. It encodes for ______ biosynthesis.

A. adenine
B. uracil
C. guanine
D. cytosine
Answer» C. guanine
Discussion
7.

YEAST_EPISOMAL_PLASMIDS_HAVE_THE_FOLLOWING_FEATURE??$

A. They contain ARS and CEL both
B. They contain ARS but not CEL
C. They contain CEL but not ARS
D. CEL is necessarily present but ARS may or may not be present
Answer» C. They contain CEL but not ARS
Discussion
8.

Cis-acting REP 3 sequence is present in Yeast episomal plasmids. Choose the statement which holds true for it?$

A. It is present along with chromosomal centromeric sequence
B. It is the site of action of proteins that help in partitioning
C. It gives less stability to that of YCs
D. The copy number is low than that of YCs
Answer» C. It gives less stability to that of YCs
Discussion
9.

CEN4 in YCp50 is an example of ______?

A. tetracycline resistant gene
B. ampicillin resistant gene
C. chromosomal centromeric sequence
D. autonomously replicating sequence
Answer» D. autonomously replicating sequence
Discussion
10.

Yeast centromeric plasmid (YCp50) contains ampicillin and tetracycline resistant gene. Is the given statement true or false?

A. True
B. False
Answer» B. False
Discussion
11.

The replicating plasmid vectors in yeast can be divided broadly into how many categories?

A. 1
B. 2
C. 3
D. 4
Answer» C. 3
Discussion
12.

Cells mutant in _____ gene can be selected by using 5-fluoro orotic acid, which is turned into ____ products by wild type protein.

A. SUP4, toxic
B. SUP4, non-toxic
C. URA3, toxic
D. URA3, non-toxic
Answer» D. URA3, non-toxic
Discussion
13.

Cells that have lost SUP4 gene acquire _____ pigment and are visibly distinguishable from others.

A. red
B. blue
C. green
D. pink
Answer» B. blue
Discussion
14.

Phosphoribosyl amino-imidazole carboxylase is a component of _______ biosynthesis pathway.

A. pyrimidine
B. purine
C. both purine and pyrimidine
D. only adenine
Answer» C. both purine and pyrimidine
Discussion
15.

Loss of SUP4 abolishes canavanine uptake, it is arginine analogue. Is the given statement true or false?

A. True
B. False
Answer» B. False
Discussion
16.

CAN1 gene encodes a permease that causes uptake of _________ analogue.

A. toxic guanidine
B. toxic arginine
C. non-toxic guanidine
D. non-toxic arginine
Answer» C. non-toxic guanidine
Discussion
17.

One of the commonly used markers is URA3 gene. It encodes for ______ biosynthesis.

A. adenine
B. uracil
C. guanine
D. cytosine
Answer» C. guanine
Discussion