MCQOPTIONS
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MCQOPTIONS
This section includes 163 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 51. |
Species which can grow on _______ as a carbon source are called as methylotropic. |
| A. | methylase |
| B. | methanol |
| C. | methane |
| D. | methanoic acid |
| Answer» C. methane | |
| 52. |
The promoter from fbp1+ gene is suppressed in the presence of glucose. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 53. |
If the embryo is at one-cell stage then it is found in ____________ |
| A. | ovary |
| B. | oviduct |
| C. | uterus |
| D. | either ovary or uterus |
| Answer» C. uterus | |
| 54. |
Transformed cells in the case of YAC can be selected by the presence of both URA3 and TRP1 genes. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 55. |
The disease crown gall is caused by which bacteria? |
| A. | Agrobacterium tumefaciens |
| B. | Agrobacterium rhizogenes |
| C. | Agrobacterium tumefaciens & rhizogenes bacterium causes the disease crown gall |
| D. | Any bacteria belonging to genera Rhizobium |
| Answer» B. Agrobacterium rhizogenes | |
| 56. |
DNA replication of the AcNPV takes 6 hours after infection. After how many hours of infection new virus particles are produced by budding? |
| A. | 8 hrs |
| B. | 10 hrs |
| C. | 12 hrs |
| D. | 16 hrs |
| Answer» C. 12 hrs | |
| 57. |
Binary vector systems can also be used for transferring the DNA via agrobacterium. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 58. |
If a small intermediate vector system is used along with a selectable marker, then it is called as __________ |
| A. | fusion plasmids |
| B. | hybrid plasmids |
| C. | co-integrative plasmids |
| D. | complex plasmids |
| Answer» D. complex plasmids | |
| 59. |
Wild type flies have crimson red eyes whereas rosy mutant have brick-red eyes. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» C. May be True or False | |
| 60. |
If the DNA is linearized, what is the effect on the recombination frequency? |
| A. | The recombination frequency decreases |
| B. | The recombination frequency increases |
| C. | The recombination may increase or decrease depending on the amount of DNA |
| D. | There is no effect on the recombination frequency |
| Answer» C. The recombination may increase or decrease depending on the amount of DNA | |
| 61. |
Histidinol dehydrogenase allows synthesis of histidine from exogenous histanol. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 62. |
Vaccinia virus produces its own ______ which is functional in _____ of infected cells where the virus also replicates. |
| A. | DNA polymerase, nucleus |
| B. | DNA polymerase, cytoplasm |
| C. | RNA polymerase, cytoplasm |
| D. | RNA polymerase, nucleus |
| Answer» D. RNA polymerase, nucleus | |
| 63. |
Choose the incorrect statement for YAC vectors. |
| A. | The YAC molecule is approximately 10 kb in size |
| B. | It contains both yeast origin of replication and prokaryotic origin of replication |
| C. | It doesn’t contain ampicillin resistant gene |
| D. | It contains TEL sequence |
| Answer» D. It contains TEL sequence | |
| 64. |
The enzyme used for breaking down of sucrose into glucose and fructose is called as _______________ |
| A. | sucrase |
| B. | glucanase |
| C. | maltase |
| D. | invertase |
| Answer» E. | |
| 65. |
Syncytium is a layer of ______ that have not been separated into individual cells. |
| A. | nuclei |
| B. | mitochondria |
| C. | cytoplasm |
| D. | nuclei and cytoplasm |
| Answer» B. mitochondria | |
| 66. |
What is the function of onc genes in TDNA? |
| A. | Tumour suppressing potential |
| B. | Tumour inducing potential |
| C. | Either tumour inducing or suppressing depending on the conditions |
| D. | Act as replicative genes |
| Answer» C. Either tumour inducing or suppressing depending on the conditions | |
| 67. |
In Drosophila, only nuclei cells give rise to germline cells. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 68. |
If gene of interest is inserted into protoplasts but the transformation is not stable, then it is called as __________ expression systems. |
| A. | permanent |
| B. | temporary |
| C. | transient |
| D. | unstable |
| Answer» D. unstable | |
| 69. |
Lesch-Nyhan syndrome is caused by the deficiency of the enzyme hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Cells deficient in HGPRT die in a medium containing which of the following? |
| A. | Hypoxanthine and thymidine |
| B. | Thymidine |
| C. | Aminopterin and thymidine |
| D. | Hypoxanthine, thymidine and aminopterin (HAT medium) |
| Answer» E. | |
| 70. |
Transgenic Drosophila can be created by microinjection of DNA into the embryos. These embryos are at which stage of development? |
| A. | One-cell stage |
| B. | Pre-blastoderm stage |
| C. | Blastoderm |
| D. | Morula |
| Answer» C. Blastoderm | |
| 71. |
The host should ____ P elements, these elements lead to _______ |
| A. | not have, instability |
| B. | have, stability |
| C. | not have, increase the time taken for integration |
| D. | have, reduces the time taken for integration |
| Answer» B. have, stability | |
| 72. |
The ARG8m gene which produces an enzyme for arginine biosynthesis is located in _______ and is of ______ origin. |
| A. | mitochondrial, nuclear |
| B. | nuclear, mitochondrial |
| C. | nuclear, nuclear |
| D. | mitochondrial, mitochondrial |
| Answer» B. nuclear, mitochondrial | |
| 73. |
The size of the viral genome is large and thus poses a problem. This problem can be resolved by using ____________ |
| A. | transfer vector |
| B. | co-integration plasmid |
| C. | hybrid vector |
| D. | fusion vector |
| Answer» B. co-integration plasmid | |
| 74. |
A wide range of host cell lines are available and commonly used human cell lines are obtained from ___________ |
| A. | kidney |
| B. | liver |
| C. | lymphoblast from leukaemia patient |
| D. | both kidney and lymphoblast from a leukaemia patient |
| Answer» E. | |
| 75. |
The polyhedrin and p10 protein are produced in ____________ amount and other genes are expressed in _________ amount. |
| A. | low, high |
| B. | high, high |
| C. | high, low |
| D. | low, low |
| Answer» D. low, low | |
| 76. |
The virus particles are held together by polyhedron and p10 protein. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 77. |
How many types of the chloroplast are there in Chlamydomonas? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» B. 2 | |
| 78. |
Puromycin is a protein synthesis inhibitor. It is conferred by _______ gene. |
| A. | streptococcal |
| B. | bacilovirus |
| C. | streptomyces |
| D. | both streptococcal and streptomyces |
| Answer» D. both streptococcal and streptomyces | |
| 79. |
Gram-positive Bacillus, Streptococcus and Streptomyces include species that don’t exhibit natural competence. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» C. May be True or False | |
| 80. |
Cells deficient in thymidine kinase (TK) are also killed in HAT medium. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 81. |
The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is? |
| A. | Deletion of the packaging signal in the helper genome |
| B. | Deletion of multiple parts of the helper genome |
| C. | Division of the helper genome into several pieces |
| D. | All of the mentioned |
| Answer» E. | |
| 82. |
Basically, there are how many methods for introduction of DNA into the bacterial cells? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» D. 4 | |
| 83. |
Which of the bacteria are used as hosts? |
| A. | Gram-positive |
| B. | Gram-negative |
| C. | Both gram positive and negative are preferred equally |
| D. | Both can be used but gram positive is preferred |
| Answer» E. | |
| 84. |
How many deletion variations of GAL1 promoter are there? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 85. |
Natural transformation can be relieved by the use of protoplasts, in the presence of osmotic buffer and polyethylene glycol. What are protoplasts? |
| A. | Protoplasts are the cells from which cell membrane has been removed |
| B. | Protoplasts are the cells from which cell wall has been removed |
| C. | Protoplasts are the cells from which vacuole has been removed |
| D. | Protoplasts are the cells from which golgi bodies are removed |
| Answer» C. Protoplasts are the cells from which vacuole has been removed | |
| 86. |
35S promoter is obtained from _________ |
| A. | Tobacco mosaic virus |
| B. | Cauliflower mosaic virus |
| C. | Agrobacterium |
| D. | Arabdopsis |
| Answer» C. Agrobacterium | |
| 87. |
Competence is determined by the excretion into growth medium of _______ & _________ proteins called as competence proteins. |
| A. | cellular & high molecular weight |
| B. | cellular & low molecular weight |
| C. | extracellular & low molecular weight |
| D. | extracellular & high molecular weight |
| Answer» D. extracellular & high molecular weight | |
| 88. |
CAN1 gene encodes a permease that causes uptake of _________ analogue. |
| A. | toxic guanidine |
| B. | toxic arginine |
| C. | non-toxic guanidine |
| D. | non-toxic arginine |
| Answer» C. non-toxic guanidine | |
| 89. |
In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _____ of tetracycline to the control sequence and _______ transcription. |
| A. | presence, activates |
| B. | absence, activates |
| C. | absence, inactivates |
| D. | presence, inactivates |
| Answer» D. presence, inactivates | |
| 90. |
Integration events may be insertional involving _____ crossover or replacement involving _____ crossovers. |
| A. | single, single |
| B. | double, double |
| C. | double, single |
| D. | single, double |
| Answer» E. | |
| 91. |
Chlamyodomonas reinhardtii is a green _________ algae. |
| A. | unicellular |
| B. | di-cellular |
| C. | multicellular |
| D. | either unicellular or di-cellular |
| Answer» B. di-cellular | |
| 92. |
Cells whose contents have been removed and replaced, by swelling and shrinking in solutions of suitable osmotic strength are called as ____________ |
| A. | protoplast |
| B. | ghosts |
| C. | shrunken cells |
| D. | vacuole |
| Answer» C. shrunken cells | |
| 93. |
Caenorhabditis elegans is a model organism of great importance in biological systems. It is a/an _________ |
| A. | algae |
| B. | parasite |
| C. | fungi |
| D. | nematode |
| Answer» E. | |
| 94. |
Which of the plant growth regulators are produced by TDNA? |
| A. | Salicyclic acid |
| B. | Cytokinin |
| C. | Cytokinin and Auxin |
| D. | Jasmonic Acid |
| Answer» D. Jasmonic Acid | |
| 95. |
Atrazine is a herbicide and it acts on _________ |
| A. | reaction centre in photosystemI |
| B. | reaction centre in photosytemII |
| C. | reaction centre in both the photosystems |
| D. | neither of the photosystems |
| Answer» C. reaction centre in both the photosystems | |
| 96. |
The region which is transferred from bacterium to the nucleus of the plant cell is called as T-DNA. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 97. |
Baciullus thuringiensis is used for the production of toxins which can be used as _________ |
| A. | insecticides |
| B. | pesticides |
| C. | germicides |
| D. | fungicides |
| Answer» B. pesticides | |
| 98. |
Which of the following promoter is having high tissue specificity? |
| A. | 35S promoter |
| B. | nos promoter |
| C. | 35S & nos promoters are having high specificity |
| D. | neither of the promoter is having high specificity |
| Answer» E. | |
| 99. |
Chloroplast can be transferred through pollen in all crops. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» C. May be True or False | |
| 100. |
The replicating plasmid vectors in yeast can be divided broadly into how many categories? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |