 
			 
			MCQOPTIONS
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				This section includes 16 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. | If the cells containing plasmids are infected with helper phage, which type of DNA can be produced, packaged and secreted into the medium? | 
| A. | Single stranded DNA | 
| B. | Double stranded DNA | 
| C. | Both single and double stranded DNA | 
| D. | Circular DNA | 
| Answer» D. Circular DNA | |
| 2. | If transcription should not be carried out beyond the insert in the vector, then it should be linearized. | 
| A. | True | 
| B. | False | 
| Answer» B. False | |
| 3. | By selecting the appropriate polymerase to activate the promoter ________ can be carried out _______ | 
| A. | transcription, regardless of orientation | 
| B. | transcription, only in one orientation | 
| C. | translation, regardless of orientation | 
| D. | translation, only in one orientation | 
| Answer» B. transcription, only in one orientation | |
| 4. | Promoters are generally used after isolation from ___________ | 
| A. | bacteriophage T7 | 
| B. | bacteriophage SP6 | 
| C. | baceriophage Mu | 
| D. | both bacteriophage T7 and SP6 | 
| Answer» E. | |
| 5. | Isolation of RNA can be carried out easily from bacterial cells. | 
| A. | True | 
| B. | False | 
| Answer» C. | |
| 6. | Cloning vectors designed for the purpose of synthesis of RNA and proteins are known as ___________ | 
| A. | Cloning vectors | 
| B. | RNA vectors | 
| C. | Bacteriophage vectors | 
| D. | Expression vectors | 
| Answer» E. | |
| 7. | IF_THE_CELLS_CONTAINING_PLASMIDS_ARE_INFECTED_WITH_HELPER_PHAGE,_WHICH_TYPE_OF_DNA_CAN_BE_PRODUCED,_PACKAGED_AND_SECRETED_INTO_THE_MEDIUM??$ | 
| A. | Single stranded DNA | 
| B. | Double stranded DNA | 
| C. | Both single and double stranded DNA | 
| D. | Circular DNA | 
| Answer» D. Circular DNA | |
| 8. | If transcription should not be carried out beyond the insert in the vector, then it should be linearized. Is the given statement true or false? | 
| A. | True | 
| B. | False | 
| Answer» B. False | |
| 9. | Capping can be introduced by the use of cap analogue. Which of the statement is true? | 
| A. | Cap analogue can be introduced at the end of the transcript | 
| B. | Cap analogue can be introduced at the start of the transcript | 
| C. | Cap analogue can be introduced anywhere in the transcript | 
| D. | Cap analogue can be introduced both at the end and starting of the transcript | 
| Answer» C. Cap analogue can be introduced anywhere in the transcript | |
| 10. | Guanylyl transferase and GTP are used for? | 
| A. | Transcription | 
| B. | Translation | 
| C. | Capping of the message | 
| D. | Packaging | 
| Answer» D. Packaging | |
| 11. | By selecting the appropriate polymerase to activate the promoter, ________ can be carried out _______ | 
| A. | transcription, regardless of orientation | 
| B. | transcription, only in one orientation | 
| C. | translation, regardless of orientation | 
| D. | translation, only in one orientation | 
| Answer» B. transcription, only in one orientation | |
| 12. | Promoters are generally used after isolation from: | 
| A. | bacteriophage T7 | 
| B. | bacteriophage SP6 | 
| C. | baceriophage Mu | 
| D. | both bacteriophage T7 and SP6 | 
| Answer» E. | |
| 13. | Isolation of RNA can be carried out easily from bacterial cells. Is the given statement true or false? | 
| A. | True | 
| B. | False | 
| Answer» C. | |
| 14. | RNA can be synthesized by using vector. A vector with _______ is used and further through ________ RNA is isolated. | 
| A. | origin of replication, translation | 
| B. | promoter, transcription | 
| C. | promoter, translation | 
| D. | origin of replication, transcription | 
| Answer» C. promoter, translation | |
| 15. | For studying processes such as splicing and cleavage, RNA is required. Choose the correct statement for this. | 
| A. | A mixture of different types of RNA is required | 
| B. | Two types of RNA are required | 
| C. | A few contaminating proteins are required | 
| D. | Sodium hydroxide is required | 
| Answer» D. Sodium hydroxide is required | |
| 16. | Cloning vectors designed for the purpose of synthesis of RNA and proteins are known as: | 
| A. | Cloning vectors | 
| B. | RNA vectors | 
| C. | Bacteriophage vectors | 
| D. | Expression vectors | 
| Answer» E. | |