MCQOPTIONS
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MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Cell Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following is a commonly used label in blotting techniques? |
| A. | vimentin |
| B. | biotin |
| C. | avidin |
| D. | streptomycin |
| Answer» C. avidin | |
| 2. |
Which of the following molecules can be analyzed using a northern blot? |
| A. | RNA |
| B. | Carbohydrates |
| C. | Proteins |
| D. | DNA |
| Answer» B. Carbohydrates | |
| 3. |
In which of the following techniques, the DNA is labeled using complementary sequences and then analyzed using autoradiography? |
| A. | Western blotting |
| B. | Southern blotting |
| C. | Isopycnic centrifugation |
| D. | Spectrophotometry |
| Answer» C. Isopycnic centrifugation | |
| 4. |
The procedure by which DNA gel is transferred to a membrane is termed as ___________________ |
| A. | blotting |
| B. | centifugation |
| C. | electrophoresis |
| D. | paper chromatography |
| Answer» B. centifugation | |
| 5. |
Nucleic acid hybridization is used to identify ________________________ |
| A. | RNAs |
| B. | DNAs |
| C. | Complementary base sequences |
| D. | Proteins |
| Answer» D. Proteins | |
| 6. |
Which technique can be used to separate 15N and 14N? |
| A. | Rate-zonal centrifugation |
| B. | Isopycnic centrifugation |
| C. | HPLC |
| D. | Mass spectrometry |
| Answer» C. HPLC | |
| 7. |
Equilibrium centrifugation is also known as ___________________ centrifugation. |
| A. | isotonic |
| B. | isopycnic |
| C. | gradient |
| D. | sucrose |
| Answer» C. gradient | |
| 8. |
In rate-zonal sedimentation, the nucleic acids are separated base on __________________ |
| A. | nucleotide length |
| B. | relative charge |
| C. | relative mass |
| D. | mass-to-charge ratio |
| Answer» B. relative charge | |
| 9. |
One Svedberg unit is equivalent to 10-13 ______________ |
| A. | hours |
| B. | minutes |
| C. | seconds |
| D. | milli-seconds |
| Answer» D. milli-seconds | |
| 10. |
Which technique is used to separate nucleic acids of size greater than 25 kb? |
| A. | SDS-PAGE |
| B. | Pulsed-field electrophoresis |
| C. | 2D- gel electrophoresis |
| D. | Isoelectric focusing |
| Answer» C. 2D- gel electrophoresis | |
| 11. |
Which type of gel is used for large nucleic acids? |
| A. | acrylamide |
| B. | cellulose |
| C. | agarose |
| D. | sephadex |
| Answer» D. sephadex | |
| 12. |
Phenol is an active protein denaturant. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 13. |
Which of the following is used to precipitate the nucleic acids? |
| A. | glycerol |
| B. | ethanol |
| C. | methanol |
| D. | ethylene |
| Answer» C. methanol | |
| 14. |
In purification steps of nucleic-acids, phenol is used for _______________________ |
| A. | deproteinization |
| B. | denaturation |
| C. | lowering pH |
| D. | lowering viscosity |
| Answer» B. denaturation | |
| 15. |
Which of the following is used to lyse the nucleus and release the DNA? |
| A. | sodium dodecyl sulfate |
| B. | ammonium sulfate |
| C. | ferric phosphate |
| D. | fluorine |
| Answer» B. ammonium sulfate | |