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This section includes 6 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
1. |
It is easier to subclone a restriction fragment if it belongs to? |
A. | small gene |
B. | large gene |
C. | prokaryotic organism |
D. | eukaryotic organism |
Answer» C. prokaryotic organism | |
2. |
If mixed oligonucleotides are used, it is regarded as ______________ |
A. | mixed mutagenesis |
B. | multiple mutagenesis |
C. | cassette mutagenesis |
D. | polymutagenesis |
Answer» D. polymutagenesis | |
3. |
Several different mutations can be induced at one site. |
A. | True |
B. | False |
Answer» B. False | |
4. |
Once the double stranded molecule with the mutation is introduced into E. coli for replication, how many types of molecules are produced? |
A. | 1 |
B. | 2 |
C. | 3 |
D. | 4 |
Answer» C. 3 | |
5. |
Which of the following statement is incorrect for the synthesis of the second strand? |
A. | The oligonucleotide is acting as a primer for the synthesis of the second strand |
B. | DNA polymerase and dNTPs are added for synthesis |
C. | The polymerase should have 5 -3 exonuclease activity |
D. | A polymerase having 5 -3 exonuclease activity would degrade the primer that carries the mutant sequence |
Answer» D. A polymerase having 5 -3 exonuclease activity would degrade the primer that carries the mutant sequence | |
6. |
If the template is double stranded, they need to be separated before annealing of oligonucleotide. |
A. | True |
B. | False |
Answer» B. False | |