Explore topic-wise MCQs in Genetic Engineering.

This section includes 12 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

Generally, amplification is carried out between the PCR primers. But if amplification is carried out outside the primers, it is called as __________

A. Inverse PCR
B. Circular PCR
C. Non-conventional PCR
D. In-situ PCR
Answer» B. Circular PCR
Discussion
2.

Topoisomerse I is also used for cloning of PCR product at times. Which of the statement holds true for such type of cloning?

A. The restriction site is induced into the vector and the topisomerase enzyme is induced into the PCR primers
B. The topoismerase I is used for cutting both the strands
C. The induction of topoisomerase enzyme is done into the vector in the case it is very small in size
D. The restriction site is induced into the PCR primers and the topoisomerase enzyme is induced into the vector
Answer» E.
Discussion
3.

Which of the following statement is incorrect regarding the cloning of PCR products?

A. In cloning via restriction enzymes, restriction sites are induced before amplification is carried out
B. The restriction sites are induced in the primers before annealing
C. The intermediate molecules are having restriction sites at both ends
D. The amplified molecules can be cut at both the ends by appropriate enzymes
Answer» D. The amplified molecules can be cut at both the ends by appropriate enzymes
Discussion
4.

How can PCR product be cloned into a vector?

A. It can be done only when PCR products are blunt-ended
B. It can be done only by restriction enzyme digestion
C. Both the methods can be used
D. The blunt-ended approach is favoured
Answer» C. Both the methods can be used
Discussion
5.

The process of inserting an amplified PCR product in a vector for cloning is known as __________

A. making library
B. insertion
C. making a hard copy
D. making a PCR based vector
Answer» D. making a PCR based vector
Discussion
6.

If two successive PCRs are carried out, in which PCR there are chances of having a non-specific product?

A. First PCR
B. Second PCR
C. Both the PCRs
D. It depends on the annealing temperature
Answer» B. Second PCR
Discussion
7.

If two successive PCR are carried out, it is called as __________

A. Touch-down PCR
B. Hot-start PCR
C. Combined PCR
D. Nested PCR
Answer» E.
Discussion
8.

Touch-down PCR is another modification. Its characteristics include:

A. Lowering the temperature for primer annealing
B. Primer annealing is done at higher temperatures initially
C. The temperature is abruptly reduced in the second cycle
D. In earlier cycles less stringent conditions are there and in later cycles, more stringent conditions are there
Answer» C. The temperature is abruptly reduced in the second cycle
Discussion
9.

The primer annealing temperature is often very low from the maximum temperature. This low temperature leads to some mismatches.

A. True
B. False
Answer» B. False
Discussion
10.

An alternative to adding polymerase at later stage is __________

A. Make the polymerase inactive by binding it to an antibody
B. Introduce the polymerase or Magnesium in clay beads
C. Make the polymerase inactive by attaching groups which cause stearic inhindrance
D. Introduction of polymerase or Magnesium in plastic wires
Answer» B. Introduce the polymerase or Magnesium in clay beads
Discussion
11.

Hot-start PCR is a modification of PCR. Which of the following is not corresponding to it?

A. The basis is that extension is not started until the first cycle reaches its maximum temperature
B. The polymerase is added after the first cycle has reached its maximum temperature or melting temperature
C. It is satisfactory for small number of samples
D. It leads to generation of non specific products
Answer» E.
Discussion
12.

What are the possibilities which can occur until the temperature has reached for primer annealing?

A. Extension doesn’t starts until the appropriate temperature is reached
B. Extension may start even when the temperature is low
C. At low temperature, there is specific annealing of primer taking place
D. There are more specific products which are generated
Answer» C. At low temperature, there is specific annealing of primer taking place
Discussion