MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 61 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 51. |
The inactivation of endogenous genes may also be helpful in directing the expression of the mutated gene in the absence of background expression of wild type gene. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 52. |
Small deletions at a restriction site can be generated by cutting and degrading the ________ with an exonuclease. |
| A. | double stranded ended |
| B. | circular molecules |
| C. | single stranded ends |
| D. | supercoiled molecules |
| Answer» D. supercoiled molecules | |
| 53. |
Restriction site can also be introduced by oligonucleotide directed mutagenesis of a region that is _______ and ______ to the original restriction site. |
| A. | not only similar, also exactly same |
| B. | similar, not same |
| C. | not similar, is entirely different |
| D. | different, near |
| Answer» C. not similar, is entirely different | |
| 54. |
If a functional gene is disrupted while disrupting a restriction site _______ is created. |
| A. | frameshift mutation |
| B. | point mutation |
| C. | either of the mutations |
| D. | any other kind of mutation |
| Answer» B. point mutation | |
| 55. |
When a series of deleted regions are replaced by some other DNA fragment of equal length, then it is known as: |
| A. | linker scanning |
| B. | generation of deletions |
| C. | mutation |
| D. | replacement |
| Answer» B. generation of deletions | |
| 56. |
Additional restriction sites can be introduced near the existing restriction site by mutagenesis. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 57. |
If there are multiple restriction sites for an enzyme in a given molecule, digestion is carried out in many steps. The initial digestion should be? |
| A. | complete |
| B. | partial |
| C. | carried out by a different enzyme then that the restriction site |
| D. | carried out by a mixture of restriction enzymes |
| Answer» C. carried out by a different enzyme then that the restriction site | |
| 58. |
At times, the gene which is cloned is not well known for the protein encoded by it. To access the function, the endogenous gene for the mutant strain is inactivated. This technique is called as ___________ |
| A. | reverse genetics |
| B. | protein engineering |
| C. | mutation |
| D. | location of function |
| Answer» B. protein engineering | |
| 59. |
Direct alteration of particular parts of protein as a way of probing the relationship between structure and function is termed as: |
| A. | genetic engineering |
| B. | protein engineering |
| C. | alteration of protein function |
| D. | structure engineering |
| Answer» C. alteration of protein function | |
| 60. |
In the case of deletion of a restriction site, it should be cleaved with the same restriction enzyme. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 61. |
Approaches for creating mutations can be divided into how many types? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |