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This section includes 6 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
1. |
If colEI derived origin of replication is replaced by the origin of replication of F plasmid, it is called as: |
A. | phagemid |
B. | F cosmid |
C. | plasmid |
D. | fosmid |
Answer» E. | |
2. |
What happens once the cosmid enters the E. coli cells? |
A. | There is strict size selection inside E. coli cells |
B. | Partial deletion may take place |
C. | The tendency of deletion may is increased by using low copy number |
D. | The tendency of deletion can t be altered |
Answer» C. The tendency of deletion may is increased by using low copy number | |
3. |
Which size of the insert is accepted by the cosmids? |
A. | 10-20 kbp |
B. | 35-45 kbp |
C. | 50-60 kbp |
D. | 100-120 kbp |
Answer» C. 50-60 kbp | |
4. |
Once cosmids are inside the E.coli cells, they don t generate phage but are propagated as plasmids. |
A. | True |
B. | False |
Answer» B. False | |
5. |
Which of the following doesn t takes place after replication? |
A. | The single stranded sequence is generated |
B. | It is circularized to form closed single stranded molecule |
C. | It may circularize or remain linear |
D. | The double stranded molecule can be synthesized by cellular DNA synthesis |
Answer» D. The double stranded molecule can be synthesized by cellular DNA synthesis | |
6. |
Choose the correct statement for the lambda ZAP vector. |
A. | It is not based on bacteriophage lambda |
B. | It contains a region that can be excised in vivo |
C. | The excision leads to the formation of bluescript plasmids and it contains an initiator region only |
D. | The multiple cloning site is not flanked by the initiator and the terminator region |
Answer» C. The excision leads to the formation of bluescript plasmids and it contains an initiator region only | |