MCQOPTIONS
Saved Bookmarks
MCQOPTIONS
This section includes 115 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 101. |
For the synthesis of the second DNA strand, incorporated oligo-dT is of no use. Why? |
| A. | It is so because it is unstable |
| B. | It is so because it is at the 3’ end of the template molecule |
| C. | It is so because it would be unable to initiate replication |
| D. | It is so because it detaches itself after first strand synthesis |
| Answer» D. It is so because it detaches itself after first strand synthesis | |
| 102. |
What is the basis of RNaseH method? |
| A. | It is based on RNA synthesis by DNA strand |
| B. | It is based on complementary DNA synthesis by RNA strand through reverse transcriptase |
| C. | It is based on complementary DNA synthesis by RNA strand through RNaseH enzyme |
| D. | It is based on getting double strand RNA from a single strand |
| Answer» C. It is based on complementary DNA synthesis by RNA strand through RNaseH enzyme | |
| 103. |
The first step for RNaseH method is to anneal a chemically synthesized oligo-dT primer to the 3’ polyA tail of RNA. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't Say |
| Answer» B. False | |
| 104. |
At times streptavidin is used in place of cellulose. Choose the correct statement for this alternative. |
| A. | oligo-dT streptavidin conjugate can be extracted by magnetic beads |
| B. | The magnetic beads are attached with activin |
| C. | Recovery is not based on the magnetic properties of the beads |
| D. | The interaction between polyA tail and the oligo-dT is reduced |
| Answer» B. The magnetic beads are attached with activin | |
| 105. |
Choose the correct with respect to RNA molecules. |
| A. | They are less labile than DNA molecules |
| B. | The 2’ hydroxyl group of ribose group decreases the activity |
| C. | There is no less of activity while boiling |
| D. | Baking of glassware, treatment with UV can be used for protection against degradation |
| Answer» E. | |
| 106. |
DURING_STORAGE_THERE_ARE_CHANCES_OF_DEGRADATION_OF_DNA._LARGER_FRAGMENTS_ARE_HAVING_MORE_CHANCES_OF_BEING_GOT_LOST._THE_GIVEN_STATEMENT_IS_TRUE_OR_FALSE??$ |
| A. | True |
| B. | False |
| Answer» B. False | |
| 107. |
LIBRARIES_CONSTRUCTED_IN_PLASMID_VECTORS_CAN_BE_KEPT_AS:?$ |
| A. | plasmid containing cells |
| B. | naked DNA |
| C. | both plasmid containing cells and naked DNA |
| D. | naked DNA is preferred over plasmid containing cells |
| Answer» D. naked DNA is preferred over plasmid containing cells | |
| 108. |
Libraries using phage cloning vectors are often kept as? |
| A. | unpackaged phage |
| B. | packaged phage |
| C. | both packaged and unpackaged phage |
| D. | both packaged and unpackaged phage are used but packaged is favoured |
| Answer» C. both packaged and unpackaged phage | |
| 109. |
Vector and insert are mixed, ligated and packaged and introduced into the host by: |
| A. | transformation |
| B. | transduction |
| C. | infection |
| D. | transformation and infection both |
| Answer» E. | |
| 110. |
To avoid ligation of separate DNA fragments, which of the enzyme is used? |
| A. | phosphatase |
| B. | kinase |
| C. | ligase |
| D. | endonuclease |
| Answer» B. kinase | |
| 111. |
Complete digestion is preferred over partial digestion for the fragmentation of DNA. The given statement is true or false? |
| A. | True |
| B. | False |
| Answer» C. | |
| 112. |
The various steps for construction of libraries are: |
| A. | Fragmentation of DNA |
| B. | Isolation of genomic DNA |
| C. | Amplification |
| D. | Ligation and introduction to the host |
| Answer» C. Amplification | |
| 113. |
Choose the incorrect statement for preparation of genomic libraries. |
| A. | The first step is isolation of genomic DNA |
| B. | Physical damage to the DNA should be avoided |
| C. | If a nuclear DNA library is to be constructed, organelle DNA is to be removed |
| D. | For the construction of organelle library, organelle DNA is purified from the nuclear DNA |
| Answer» D. For the construction of organelle library, organelle DNA is purified from the nuclear DNA | |
| 114. |
Choose the correct statement for genomic libraries. |
| A. | Genomic libraries include the representation of the whole genome of the organism |
| B. | Sequences such as telomeres are also represented |
| C. | Telomeres can be readily cloned |
| D. | The larger the size of insert of genomic DNA in recombinants, the more is the number of recombinants required to represent the genome in the library |
| Answer» B. Sequences such as telomeres are also represented | |
| 115. |
Libraries can broadly be classified into how many types? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |