MCQOPTIONS
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MCQOPTIONS
This section includes 115 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 51. |
Choose the incorrect statement for the preparation of genomic libraries. |
| A. | The first step is the isolation of genomic DNA |
| B. | Physical damage to the DNA should be avoided |
| C. | If a nuclear DNA library is to be constructed, organelle DNA is to be removed |
| D. | For the construction of organelle library, organelle DNA is purified from the nuclear DNA |
| Answer» D. For the construction of organelle library, organelle DNA is purified from the nuclear DNA | |
| 52. |
In case if molecules smaller than the fragments required for making a full genomic library are used for making a collection. This collection is called as ___________ |
| A. | library |
| B. | shelf |
| C. | small library |
| D. | mini library |
| Answer» C. small library | |
| 53. |
Any cDNA library would represent a fraction of RNA species of an organism. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't Say |
| Answer» B. False | |
| 54. |
Choose the correct statement for libraries constructed by mixing cell types differing in the sequences they are having. |
| A. | The driver and tracer are mixed in double stranded form |
| B. | Tracer is in stoichiometric excess in comparison to driver |
| C. | Tracer and driver are in the same amount |
| D. | The sequences are allowed to hybridize |
| Answer» E. | |
| 55. |
Which library should be used, genomic or cDNA when the screening is carried out by oligonucleotide probes? |
| A. | Genomic |
| B. | cDNA |
| C. | Both genomic and cDNA library can be used equivalently |
| D. | None of the libraries are preferred if oligonucleotide probes are used |
| Answer» C. Both genomic and cDNA library can be used equivalently | |
| 56. |
Libraries in which a particular sequence is present in one organism but are absent from another organism, are called as ___________ |
| A. | normalized libraries |
| B. | subtractive libraries |
| C. | selective libraries |
| D. | partial libraries |
| Answer» C. selective libraries | |
| 57. |
By synthesizing two strands separately then annealing them leads to formation of double stranded oligonucleotide. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 58. |
Libraries using phage cloning vectors are often kept as ___________ |
| A. | unpackaged phage |
| B. | packaged phage |
| C. | both packaged and unpackaged phage |
| D. | both packaged and unpackaged phage are used but packaged is favoured |
| Answer» C. both packaged and unpackaged phage | |
| 59. |
Which statement holds true for hydroxyapatite? |
| A. | It binds to single stranded molecules more tightly than double stranded molecules |
| B. | It binds to linear molecules more tightly than circular molecules |
| C. | It binds to circular molecules more tightly than linear molecules |
| D. | It binds to double stranded molecules more tightly than single stranded molecules |
| Answer» E. | |
| 60. |
If for a particular organism sequence data is available and we have to simply search in the data through a computer, then this method is called as ___________ |
| A. | annotation |
| B. | database search |
| C. | in silico |
| D. | electronic search |
| Answer» D. electronic search | |
| 61. |
Promoter-probe vectors are used often. Choose the correct statement for these vectors. |
| A. | They are used for identifying sequences that can function as promoter in vivo |
| B. | It contains a reporter gene which contains its promoter along |
| C. | The reporter gene is having a cloning site |
| D. | After insertion of DNA into the cloning site, selection of plasmids is carried out by blue white screening |
| Answer» B. It contains a reporter gene which contains its promoter along | |
| 62. |
Choose the incorrect statement for the homopolymer tailing of cDNA strands. |
| A. | Choose the incorrect statement for the homopolymer tailing of cDNA strands. |
| B. | Vector is also treated with terminal transferase and dGTPs |
| C. | The vector and cDNA can now anneal with the help of DNA ligase |
| D. | If gaps are created they can be repaired by physiological processes |
| Answer» D. If gaps are created they can be repaired by physiological processes | |
| 63. |
Choose the correct statement if the screening is carried out by screening by expression in vivo. |
| A. | The proportion of recombinants carrying gene of interest is small |
| B. | The recombinants carrying gene of interest don’t complement the host mutation |
| C. | Mutation should be affecting many genes |
| D. | Most of the products selected would be result of complementation |
| Answer» B. The recombinants carrying gene of interest don’t complement the host mutation | |
| 64. |
Sometimes the required mRNA is present in less number. So the process of increasing the representation of rare mRNAs is called as ____________ |
| A. | amplification |
| B. | normalization |
| C. | selection |
| D. | narrowing |
| Answer» C. selection | |
| 65. |
Protein-protein interactions such as in electron transport lead to activation of the reporter gene. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» C. May be True or False | |
| 66. |
Xenopus oocyte cells are also used at the time to carry out screening. Choose the incorrect statement for this procedure. |
| A. | DNA from collections is transcribed within in vitro and the transcription products are microinjected into oocytes |
| B. | The RNA is translated within oocytes |
| C. | Screening is carried out on the basis of DNA |
| D. | The screening is helpful for the proteins whose function can be easily screened such as transport of ions |
| Answer» D. The screening is helpful for the proteins whose function can be easily screened such as transport of ions | |
| 67. |
In the case of ligand binding by the expressed protein, the library can be screened by ______________ |
| A. | In the case of ligand binding by the expressed protein, the library can be screened by ______________ |
| B. | ligands if the sequence we are looking for encodes a protein specific to a ligand |
| C. | using a specific DNA sequence which can bind to the protein encoded by the sequence of interest |
| D. | all of the mentioned |
| Answer» E. | |
| 68. |
If the clone for a gene is obtained on the basis of its position in the gene map, then it is called as ___________ |
| A. | locational cloning |
| B. | positional cloning |
| C. | chromosome walking |
| D. | transposon tagging |
| Answer» C. chromosome walking | |
| 69. |
Sequences that can function as origins of replication are called as ___________ |
| A. | partial replicating sequences |
| B. | self replicating sequences |
| C. | autonomously replicating sequences |
| D. | modified replicating sequences |
| Answer» D. modified replicating sequences | |
| 70. |
The loop region is single stranded. It can be cleaved by using which enzyme? |
| A. | Exonuclease |
| B. | S1 nuclease |
| C. | RNaseH |
| D. | DNase |
| Answer» C. RNaseH | |
| 71. |
Sometimes successive rounds of screening of a genomic library are carried out and an ordered collection of clones is done in a linear fashion, then the process is called as ___________ |
| A. | chromosome jumping |
| B. | chromosome sorting |
| C. | chromosome walking |
| D. | transposon tagging |
| Answer» D. transposon tagging | |
| 72. |
Choose the incorrect statement for the method homopolymer tailing. |
| A. | The first step is the RNA: DNA hybrid synthesis |
| B. | Terminal transferase is used for the addition of nucleotides on 3’ end |
| C. | Terminal transferase adds only at DNA strands |
| D. | The DNA strand is now having known sequence at 3’ end |
| Answer» D. The DNA strand is now having known sequence at 3’ end | |
| 73. |
What is the final product of the RNaseH method? |
| A. | blunt ended dsDNA |
| B. | staggered dsDNA at both ends |
| C. | staggered dsDNA at 3’ end |
| D. | staggered dsDNA at 5’ end |
| Answer» B. staggered dsDNA at both ends | |
| 74. |
If an expression of the coding sequence is to be carried in the vitro, then which of the statement holds true? |
| A. | The extract in which the host DNA constructs are incubated is capable of translation only |
| B. | The extract in which the host DNA constructs are incubated is capable of transcription only |
| C. | The extract is made up of lysate of E. coli cells, containing RNA polymerase, ribosomes, tRNAs etc |
| D. | Non radiolabelled amino acids are used |
| Answer» D. Non radiolabelled amino acids are used | |
| 75. |
If screening is carried out by using a combination of nascent peptide and mRNA, then it is called as ________ |
| A. | nascent peptide display |
| B. | mRNA display |
| C. | ribosome display |
| D. | ribozyme display |
| Answer» D. ribozyme display | |
| 76. |
Choose the incorrect statement for prehybridization mixture. |
| A. | The function of it is to carry out specific binding |
| B. | It constitutes of non-labelled DNA of non-specific sequence which is obtained from salmon or herring testes or sperm |
| C. | It is added in a small amount |
| D. | It is carried out before the hybridization is carried out |
| Answer» B. It constitutes of non-labelled DNA of non-specific sequence which is obtained from salmon or herring testes or sperm | |
| 77. |
In ______ organisms, firstly the gene is looked into model organisms. |
| A. | eukaryotic |
| B. | prokaryotic |
| C. | both eukaryotic and prokaryotic |
| D. | large sized |
| Answer» B. prokaryotic | |
| 78. |
For two hybrid systems, activation domains can be present in different proteins also rather than being on a single protein. A sequence encodes _______ protein for which we want to find an interacting protein. |
| A. | prey |
| B. | bait |
| C. | binding |
| D. | activation |
| Answer» C. binding | |
| 79. |
The transcription domain is ________ if some of the bait and prey proteins are non-specific in nature? |
| A. | deactivated |
| B. | activated |
| C. | destroyed |
| D. | may be activated or not |
| Answer» E. | |
| 80. |
The ligand should bind to a single protein only for the screening process. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't Say |
| Answer» B. False | |
| 81. |
What is used for lysing of bacterial cells and denaturation of DNA? |
| A. | Exonuclease |
| B. | Sulphuric Acid |
| C. | Sodium Hydroxide |
| D. | Heat |
| Answer» D. Heat | |
| 82. |
Choose the correct statement if the RNA is non polydenylated. |
| A. | A collection of chemically synthesized oligonucleotides is used as primers |
| B. | They are usually tetramer |
| C. | Unequal quantities of A, G, T and C are used |
| D. | The primers attach at only specific sequences for first strand synthesis |
| Answer» B. They are usually tetramer | |
| 83. |
A times partial sequencing of cloned cDNAs is carried out. These cDNA are known as ___________ |
| A. | expressed RNA sequences |
| B. | expressed sequence tags (ESTs) |
| C. | expressed cDNA sequences |
| D. | library |
| Answer» C. expressed cDNA sequences | |
| 84. |
The first cDNA strand in 5’ RACE is tailed with oligo-dA tail. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 85. |
Libraries constructed in plasmid vectors can be kept as ___________ |
| A. | plasmid containing cells |
| B. | naked DNA |
| C. | both plasmid containing cells and naked DNA |
| D. | naked DNA is preferred over plasmid containing cells |
| Answer» D. naked DNA is preferred over plasmid containing cells | |
| 86. |
In case of promoter-probe vectors, the same or related species should be as a vector whose DNA is to be screened. |
| A. | True |
| B. | False |
| C. | May be True or False |
| D. | Can't say |
| Answer» B. False | |
| 87. |
If translation and transcription are carried out separately, then what is added to carry out translation? |
| A. | DNA polymerase |
| B. | RNA polymerase |
| C. | Taq polymerase |
| D. | Tfl polymerase |
| Answer» C. Taq polymerase | |
| 88. |
Vector and insert are mixed, ligated and packaged and introduced into the host by ___________ |
| A. | transformation |
| B. | transduction |
| C. | infection |
| D. | transformation and infection |
| Answer» E. | |
| 89. |
In the case of immunochemical screening, the position of ________ antibody is detected by ________ antibody. |
| A. | secondary, primary |
| B. | primary, secondary |
| C. | primary, tertiary |
| D. | secondary, tertiary |
| Answer» C. primary, tertiary | |
| 90. |
Choose the correct statement for RNA fractionation. |
| A. | The RNA is fractioned by size but before separating on oligo-dT cellulose |
| B. | A sucrose density gradient is used |
| C. | The RNA is applied to the top of a pre-poured gradient and during centrifugation smaller molecules move down the tube faster |
| D. | Different bands are formed according to the density in the sucrose density gradient |
| Answer» C. The RNA is applied to the top of a pre-poured gradient and during centrifugation smaller molecules move down the tube faster | |
| 91. |
At times screening is done for the protein product of DNA of interest rather than the sequence itself. How many methods are there to carry out this? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 92. |
Choose the correct statement with respect to the bacterial genome. |
| A. | They are easy to sequence |
| B. | They are difficult to sequence |
| C. | Methods such as transcription and translation are satisfactory |
| D. | Only transcription can be carried out |
| Answer» C. Methods such as transcription and translation are satisfactory | |
| 93. |
Screening is often carried out for a sequence that interacts with a protein for which already a clone is present. It is carried out in which host? |
| A. | Bacterial host |
| B. | Fungal host |
| C. | Parasitic host |
| D. | Yeast host |
| Answer» E. | |
| 94. |
Introns are ________ in nature and the sequence is _______ than exons. |
| A. | coding, less random |
| B. | non-coding, less random |
| C. | non-coding, more random |
| D. | coding, more random |
| Answer» D. coding, more random | |
| 95. |
Choose the correct statement for construction of a library subsection. |
| A. | The size of a particular restriction fragment on which the gene is located is not known |
| B. | The size of the restriction fragment can be known by carrying out southern blotting |
| C. | Another digest of the genomic DNA is carried out by a different enzyme |
| D. | DNA fragments of different size are recovered after carrying out gel electrophoresis |
| Answer» C. Another digest of the genomic DNA is carried out by a different enzyme | |
| 96. |
For cloning purposes, the intact chromosomes should be separated by ___________ |
| A. | agarose gel electrophoresis |
| B. | fluorescence- activated sorter |
| C. | polyacrylamide gel electrophoresis |
| D. | chromatography |
| Answer» C. polyacrylamide gel electrophoresis | |
| 97. |
Choose the incorrect statement for second DNA strand synthesis. |
| A. | The RNA: DNA complex is treated with the enzyme RNaseH and DNA pol |
| B. | The enzyme RNaseH is responsible for nicking the RNA strand and leaving free 3’ hydroxyl ends |
| C. | These RNA fragments can be used as primers |
| D. | There is no portion of RNA left attached to the DNA strand |
| Answer» E. | |
| 98. |
The process of examining stained chromosomes in a light microscope and removing appropriate regions with a micro-manipulator is called as ___________ |
| A. | microdissection |
| B. | chromosome sorting |
| C. | chromosome walking |
| D. | chromosome jumping |
| Answer» B. chromosome sorting | |
| 99. |
What is the second primer in the case of 5’ RACE? |
| A. | Internal primer |
| B. | Oligo-dA sequence |
| C. | Adaptor-oligo-dT primer |
| D. | Oligo-dT adaptor molecule |
| Answer» D. Oligo-dT adaptor molecule | |
| 100. |
RNaseH method and homopolymer tailing method generates blunt ended cDNA molecules. Which of the following can be used for attaching them to vector? |
| A. | Blunt ended ligation |
| B. | Addition of linkers |
| C. | Using appropriate restriction enzymes |
| D. | All the methods can be used equivalently |
| Answer» E. | |