MCQOPTIONS
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MCQOPTIONS
This section includes 18 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
For carrying out gene manipulation, the use of cultured cells is _____ transgenic organisms. |
| A. | less reliable |
| B. | more reliable |
| C. | may be less or more reliable |
| D. | is same reliable as |
| Answer» B. more reliable | |
| 2. |
If controlled inactivation of a gene is carried out and some of the consequences when inactivation of a target gene is deleterious are avoided. It is referred as ____________ |
| A. | specialized gene targeting |
| B. | controlled gene targeting |
| C. | conditional gene targeting |
| D. | specific gene targeting |
| Answer» D. specific gene targeting | |
| 3. |
The gene targeting approach produces individuals which are ______ for inactivation of the gene. |
| A. | homozygous |
| B. | heterozygous |
| C. | either only homozygous or only heterozygous |
| D. | both heterozygous and homozygous |
| Answer» C. either only homozygous or only heterozygous | |
| 4. |
It is necessary to analyze the genome of transgenic cells to confirm that the site of integration is correct. This is carried out by southern blotting. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 5. |
If a gene is inactivated by gene targeting then it is called as ____________ |
| A. | knock-in gene |
| B. | knock-out gene |
| C. | gene disruption |
| D. | insertional inactivation |
| Answer» C. gene disruption | |
| 6. |
ES cells are used in order to ensure that insertion is done at the required chromosomal location and it is called as ____________ |
| A. | gene targeting |
| B. | knocking out |
| C. | knocking in |
| D. | gene disruption |
| Answer» B. knocking out | |
| 7. |
Embryonic stem cells (ES) are isolated and are injected again into the blastocoel of a developing embryo. The embryo which develops is entirely made up of these cells only. |
| A. | True |
| B. | False |
| Answer» C. | |
| 8. |
If the embryo is at one-cell stage then it is found in ____________ |
| A. | ovary |
| B. | oviduct |
| C. | uterus |
| D. | either ovary or uterus |
| Answer» C. uterus | |
| 9. |
IF_CONTROLLED_INACTIVATION_OF_GENE_IS_CARRIED_OUT_AND_SOME_OF_THE_CONSEQUENCES_WHEN_INACTIVATION_OF_A_TARGET_GENE_IS_DELETERIOUS_ARE_AVOIDED._IT_IS_REFERRED_AS:?$ |
| A. | specialized gene targeting |
| B. | controlled gene targeting |
| C. | conditional gene targeting |
| D. | specific gene targeting |
| Answer» D. specific gene targeting | |
| 10. |
For_carrying_out_gene_manipulation,_use_of_cultured_cells_is_______transgenic_organisms.$ |
| A. | less reliable |
| B. | more reliable |
| C. | may be less or more reliable |
| D. | is same reliable as |
| Answer» B. more reliable | |
| 11. |
The gene targeting approach produces individuals which are ______ for inactivation of gene? |
| A. | homozygous |
| B. | heterozygous |
| C. | either only homozygous or only heterozygous |
| D. | both heterozygous and homozygous |
| Answer» C. either only homozygous or only heterozygous | |
| 12. |
It is necessary to analyse the genome of transgenic cells to confirm that the site of integration is correct. This is carried out by southern blotting. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» B. False | |
| 13. |
Integration events may be insertional involving _____ crossover or replacement involving _____ crossovers. |
| A. | single, single |
| B. | double, double |
| C. | double, single |
| D. | single, double |
| Answer» E. | |
| 14. |
If a gene is inactivated by gene targeting then it is called as: |
| A. | knock-in gene |
| B. | knock-out gene |
| C. | gene disruption |
| D. | insertional inactivation |
| Answer» C. gene disruption | |
| 15. |
ES cells are used in order to ensure that insertion is done at required chromosomal location and it is called as: |
| A. | gene targeting |
| B. | knocking out |
| C. | knocking in |
| D. | gene disruption |
| Answer» B. knocking out | |
| 16. |
Embryonic stem cells (ES) are isolated and are injected again into the blastocoel of a developing embryo. The embryo which develops is entirely made up of these cells only. Is the given statement true or false? |
| A. | True |
| B. | False |
| Answer» C. | |
| 17. |
Embryonic stem cells are also used for generation of transgenic organisms. They are obtained from _______ of a developing ________ |
| A. | trophoectoderm, gastrula |
| B. | trophoectoderm, blastula |
| C. | inner cell mass, blastula |
| D. | inner cell mass, gastrula |
| Answer» D. inner cell mass, gastrula | |
| 18. |
If the embryo is at one-cell stage then it is found in: |
| A. | ovary |
| B. | oviduct |
| C. | uterus |
| D. | either ovary or uterus |
| Answer» C. uterus | |