Explore topic-wise MCQs in Genetic Engineering.

This section includes 16 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

The hybrid intron is spliced from the transcript using the splice acceptor site in the vector.

A. True
B. False
Answer» B. False
Discussion
2.

Insertion of vector into intron gives rise to ________ it contains a part coming from intron and a part from ______

A. hybrid intron, exon
B. hybrid vector, exon
C. hybrid intron, target DNA sequence
D. hybrid vector, target DNA sequence
Answer» D. hybrid vector, target DNA sequence
Discussion
3.

The reporter gene in enhancer trap system is preceded by ______

A. intron
B. exon
C. promoter
D. origin of replication
Answer» B. exon
Discussion
4.

If detection of enhancers is done it is called as ______ and for detection of transcribed sequences, it is called as ______

A. enhancer trapping, promoter trapping
B. enhancer trapping, gene trapping
C. promoter trapping, gene trapping
D. promoter trapping, enhancer trapping
Answer» C. promoter trapping, gene trapping
Discussion
5.

Sequences can be cloned flanking the reporter genes using the transposon tagging approach. The use of reporter genes to identify sequences expressed in this way is called as ___________

A. trapping
B. tagging
C. identification
D. blotting
Answer» B. tagging
Discussion
6.

Desired changes are made in the structure of the promoter and is ligated onto reporter gene. The alteration can be known by measuring the activity.

A. True
B. False
Answer» B. False
Discussion
7.

INSERTION_OF_VECTOR_INTO_INTRON_GIVES_RISE_TO_________._IT_CONTAINS_A_PART_COMING_FROM_INTRON_AND_A_PART_FROM_______?$

A. hybrid intron, exon
B. hybrid vector, exon
C. hybrid intron, target DNA sequence
D. hybrid vector, target DNA sequence
Answer» D. hybrid vector, target DNA sequence
Discussion
8.

The_hybrid_intron_is_spliced_from_the_transcript_using_the_splice_acceptor_site_in_the_vector._Is_the_given_statement_true_or_false?$

A. True
B. False
Answer» B. False
Discussion
9.

The reporter gene in enhancer trap system is preceded by _____?

A. intron
B. exon
C. promoter
D. origin of replication
Answer» B. exon
Discussion
10.

For gene trap vector system, reporter gene and _____ are present. Reporter gene is _____

A. selectable marker, having promoter
B. selectable marker, promoterless
C. helper phage, promoterless
D. helper phage, having promoter
Answer» C. helper phage, promoterless
Discussion
11.

The enhancer trap system will _____ in the ______

A. not replicate independently, host
B. replicate independently, host
C. not replicate independently, vector
D. replicate independently, vector
Answer» B. replicate independently, host
Discussion
12.

If detection of enhancers is done it is called as ______ and for detection of transcribed sequences it is called as ______

A. enhancer trapping, promoter trapping
B. enhancer trapping, gene trapping
C. promoter trapping, gene trapping
D. promoter trapping, enhancer trapping
Answer» C. promoter trapping, gene trapping
Discussion
13.

Sequences can be cloned flanking the reporter genes using the transposon tagging approach. The use of reporter genes to identify sequences expressed in this way is called as:

A. trapping
B. tagging
C. identification
D. blotting
Answer» B. tagging
Discussion
14.

______ fluorescent protein should be used for determination of _______ of subcellular components.

A. One, age
B. One, location
C. More than one, location
D. More than one, age
Answer» D. More than one, age
Discussion
15.

Desired changes are made in the structure of the promoter and is ligated onto reporter gene. The alteration can be known by measuring the activity. Is the given statement true or false?

A. True
B. False
Answer» B. False
Discussion
16.

How many types of reporter gene constructs are there?

A. 1
B. 2
C. 3
D. 4
Answer» C. 3
Discussion