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This section includes 5 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
1. |
Which of the following statement is correct for the method of separation of large molecules? |
A. | The large DNA molecules can pass through the matrix in any direction, not necessarily in the direction parallel to the movement |
B. | The separation can be carried out if the fields are at right angles to each other |
C. | The time of pulse should be less than that of the reorientation time |
D. | Separation is not of the megabase level |
Answer» C. The time of pulse should be less than that of the reorientation time | |
2. |
If the molecules to be separated are larger than the size for conventional electrophoresis, which type of gels can be used? |
A. | Wedge gels |
B. | Buffer-gradient gels |
C. | Pulse field gels |
D. | Varying the amount of agarose will carry out the separation |
Answer» D. Varying the amount of agarose will carry out the separation | |
3. |
The DNA is sliced and is placed into the dialysis tube containing buffer. Further electrophoresis is performed. After it, the DNA moves out of the gel but is retained by buffer. |
A. | True |
B. | False |
Answer» B. False | |
4. |
If further electrophoresis is used for recovery of DNA from gels, the method is termed as ____________ |
A. | secondary electrophoresis |
B. | recovery electrophoresis |
C. | denaturing electrophoresis |
D. | electro-elution |
Answer» E. | |
5. |
A piece of DNA that is to be separated is crushed and soaked into the buffer. The majority of DNA diffuses, how can it be separated? |
A. | Filtration and centrifugation |
B. | Filtration or centrifugation |
C. | Allowing to sediment |
D. | By passing through a silica column |
Answer» C. Allowing to sediment | |