Explore topic-wise MCQs in Genetic Engineering.

This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

In fusion proteins, the reading frame in a vector is out of phase with the insert.

A. True
B. False
Answer» C.
2.

Termination codon is required for stopping high levels of transcription of other regions such as _______ which might interfere with ___________ of the vector.

A. origin, replication
B. end, replication
C. end, stability
D. origin, stability
Answer» E.
3.

Proteins synthesized by carrying out a translation of the vector region and continuing it in the insert region is called as _____________

A. hybrid protein
B. fusion protein
C. combination protein
D. insert protein
Answer» C. combination protein
4.

Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon.

A. True
B. False
Answer» B. False
5.

If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid.

A. few, translation
B. few, transformation
C. no, transformation
D. no, translation
Answer» D. no, translation
6.

TERMINATION_CODON_IS_REQUIRED_FOR_STOPPING_HIGH_LEVELS_OF_TRANSCRIPTION_OF_OTHER_REGION_SUCH_AS_________WHICH_MIGHT_INTERFERE_WITH______OF_THE_VECTOR.?$

A. origin, replication
B. end, replication
C. end, stability
D. origin, stability
Answer» E.
7.

In fusion proteins the reading frame in vector is out of phase with the insert. Is the given statement true or false?$

A. True
B. False
Answer» C.
8.

Which of the following is present in E. coli rrnB operon for rRNA?

A. Initiation codon
B. Termination codon
C. Both initiation and termination codon
D. Both are absent
Answer» D. Both are absent
9.

It is also possible to generate a fusion protein in which ______ is encoded by the vector.

A. N terminal
B. C terminal
C. both N and C terminal
D. middle region
Answer» C. both N and C terminal
10.

Proteins synthesized by carrying out translation of the vector region and continuing it in the insert region is called as:

A. hybrid protein
B. fusion protein
C. combination protein
D. insert protein
Answer» C. combination protein
11.

If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted.

A. N terminal
B. C terminal
C. both N & C terminal
D. middle
Answer» B. C terminal
12.

Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon. Is the given statement true or false?

A. True
B. False
Answer» B. False
13.

Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site.

A. ACG
B. AUG
C. UAC
D. CAG
Answer» C. UAC
14.

Ribosome binding site in E.coli is composed of which sequence primarily?

A. -GAGG-
B. -CTCC-
C. -ATGG-
D. -CGTT-
Answer» B. -CTCC-
15.

If overexpression of proteins takes place, they become toxic at times. In such a case, _______ colonies are recovered after ______ with the recombinant plasmid.

A. few, translation
B. few, transformation
C. no, transformation
D. no, translation
Answer» D. no, translation