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This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
1. |
In fusion proteins, the reading frame in a vector is out of phase with the insert. |
A. | True |
B. | False |
Answer» C. | |
2. |
Termination codon is required for stopping high levels of transcription of other regions such as _______ which might interfere with ___________ of the vector. |
A. | origin, replication |
B. | end, replication |
C. | end, stability |
D. | origin, stability |
Answer» E. | |
3. |
Proteins synthesized by carrying out a translation of the vector region and continuing it in the insert region is called as _____________ |
A. | hybrid protein |
B. | fusion protein |
C. | combination protein |
D. | insert protein |
Answer» C. combination protein | |
4. |
Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon. |
A. | True |
B. | False |
Answer» B. False | |
5. |
If overexpression of proteins takes place, they become toxic at times. In such a case _______ colonies are recovered after ______ with the recombinant plasmid. |
A. | few, translation |
B. | few, transformation |
C. | no, transformation |
D. | no, translation |
Answer» D. no, translation | |
6. |
TERMINATION_CODON_IS_REQUIRED_FOR_STOPPING_HIGH_LEVELS_OF_TRANSCRIPTION_OF_OTHER_REGION_SUCH_AS_________WHICH_MIGHT_INTERFERE_WITH______OF_THE_VECTOR.?$ |
A. | origin, replication |
B. | end, replication |
C. | end, stability |
D. | origin, stability |
Answer» E. | |
7. |
In fusion proteins the reading frame in vector is out of phase with the insert. Is the given statement true or false?$ |
A. | True |
B. | False |
Answer» C. | |
8. |
Which of the following is present in E. coli rrnB operon for rRNA? |
A. | Initiation codon |
B. | Termination codon |
C. | Both initiation and termination codon |
D. | Both are absent |
Answer» D. Both are absent | |
9. |
It is also possible to generate a fusion protein in which ______ is encoded by the vector. |
A. | N terminal |
B. | C terminal |
C. | both N and C terminal |
D. | middle region |
Answer» C. both N and C terminal | |
10. |
Proteins synthesized by carrying out translation of the vector region and continuing it in the insert region is called as: |
A. | hybrid protein |
B. | fusion protein |
C. | combination protein |
D. | insert protein |
Answer» C. combination protein | |
11. |
If an expression vector contains all three, viz. a controllable promoter, a ribosome binding site and an initiation codon, then a hybrid protein is produced. The _______ region of hybrid protein is encoded by vector and the rest is expressed by the sequence inserted. |
A. | N terminal |
B. | C terminal |
C. | both N & C terminal |
D. | middle |
Answer» B. C terminal | |
12. |
Expression of cloned sequences in E.coli requires a promoter, a ribosome binding site and an initiation codon. Is the given statement true or false? |
A. | True |
B. | False |
Answer» B. False | |
13. |
Translation starts at first _____ codon in the mRNA downstream from the ribosome binding site. |
A. | ACG |
B. | AUG |
C. | UAC |
D. | CAG |
Answer» C. UAC | |
14. |
Ribosome binding site in E.coli is composed of which sequence primarily? |
A. | -GAGG- |
B. | -CTCC- |
C. | -ATGG- |
D. | -CGTT- |
Answer» B. -CTCC- | |
15. |
If overexpression of proteins takes place, they become toxic at times. In such a case, _______ colonies are recovered after ______ with the recombinant plasmid. |
A. | few, translation |
B. | few, transformation |
C. | no, transformation |
D. | no, translation |
Answer» D. no, translation | |