MCQOPTIONS
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MCQOPTIONS
This section includes 12 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Methylase is useful in cloning experiments. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 2. |
What is the function of methylase? |
| A. | Addition of methyl groups to DNA |
| B. | Removal of methyl groups from DNA |
| C. | Both in removal and addition of methyl groups from DNA |
| D. | It is used in production of methane gas |
| Answer» B. Removal of methyl groups from DNA | |
| 3. |
WHAT_IS_THE_FUNCTION_OF_METHYLASE??$ |
| A. | Addition of methyl groups to DNA |
| B. | Removal of methyl groups from DNA |
| C. | Both in removal and addition of methyl groups from DNA |
| D. | It is used in production of methane gas |
| Answer» B. Removal of methyl groups from DNA | |
| 4. |
Methylase_is_useful_in_cloning_experiments._True_or_false?$ |
| A. | True |
| B. | False |
| Answer» B. False | |
| 5. |
The extent of deletions can be manipulated by controlling which of them? |
| A. | Time of incubation only |
| B. | Amount of nuclease added |
| C. | They both have a role to play in the extent of deletion |
| D. | The amount of deletion which can be carried out for a particular amount of DNA is fixed regardless of the amount of nuclease and incubation time |
| Answer» D. The amount of deletion which can be carried out for a particular amount of DNA is fixed regardless of the amount of nuclease and incubation time | |
| 6. |
Bal31 is also an enzyme which is used. Which of the following statements hold true in its context? |
| A. | It is having only 3’-5’ exonuclease activity and no endonuclease activity |
| B. | It is having only an endonuclease activity |
| C. | It is having 5’-3’ exonuclease activity |
| D. | It leads to shortening from both the ends |
| Answer» E. | |
| 7. |
How can one end be protected from the action of Exonuclease III, so that the molecule is not shortened from both the ends? |
| A. | By using Phosphorothioate nucleotide analogue |
| B. | By making both the ends double stranded in nature |
| C. | By labelling one end with a radioactive compound |
| D. | By increasing the time of exposure of the DNA molecule to the enzyme |
| Answer» B. By making both the ends double stranded in nature | |
| 8. |
What happens if a DNA molecule is treated by first Exonuclease III and then followed by treatment with S1 nuclease? |
| A. | The molecule is shortened only from 3’ en. |
| B. | The molecule is shortened only from 5’ end |
| C. | The molecule is shortened from both the ends |
| D. | Only Exonuclease acts and S1 doesn’t acts |
| Answer» D. Only Exonuclease acts and S1 doesn‚Äö√Ñ√∂‚àö√ë‚àö¬•t acts | |
| 9. |
Which of the following statements is correct regarding S1 nuclease? |
| A. | It acts on double stranded DNA |
| B. | It acts on single stranded DNA |
| C. | It acts on both types of strands |
| D. | It is obtained from E. coli |
| Answer» C. It acts on both types of strands | |
| 10. |
What is the mode of action of exonuclease III? |
| A. | Exonuclease III acts on single stranded DNA in 3’-5’ direction |
| B. | Exonuclease III acts on double stranded DNA in 5’-3’ direction |
| C. | Exonuclease III acts on single stranded DNA in 5’-3’ direction |
| D. | Exonuclease III acts on double stranded DNA in 3’-5’ direction |
| Answer» E. | |
| 11. |
How many approaches are there which can be used for exonucleolytic activity in double stranded DNA molecules? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 12. |
Which of the following statements is correct with respect to exonuclease? |
| A. | They only act on single stranded DNA molecules |
| B. | They only act on double stranded DNA molecules |
| C. | They remove a single nucleotide base at a time |
| D. | They remove nucleotide bases from the middle of polynucleotide chain |
| Answer» D. They remove nucleotide bases from the middle of polynucleotide chain | |