Choose the incorrect statement with respect to the membranes used for adhering of bacterial cells onto them?

Nitrocellulose membranes were preferred earlier

They can be handled easily without breakage

The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps:

Cells are lysed and denaturing of DNA is being carried out

Peeling of membranes carrying away bacterial cells with it

Cells are lysed and denaturing of DNA is being carried out

Hybridization with labelled DNA

Placing the membrane onto plate containing recombinant cells

The library may be plaques on a bacterial lawn and in that case it is known as plaque lift

It is the base for screening based on nucleic acid hybridization

It is also known as Grunstein-Hogness technique

It is based on the fact that which members of the library have the same sequence as the DNA probe

The library may be plaques on a bacterial lawn and in that case it is known as plaque lift

1.	NITROCELLULOSE_MEMBRANES_ARE_LESS_SENSITIVE_THAN_NYLON_MEMBRANES._IS_THE_GIVEN_STATEMENT_TRUE_OR_FALSE??$
A.	True
B.	False
Answer» C.

2.	What_is_used_for_lysing_of_bacterial_cells_and_denaturation_of_DNA?$
A.	Exonuclease
B.	Sulphuric Acid
C.	Sodium Hydroxide
D.	Heat
Answer» D. Heat

3.	Choose the incorrect statement with respect to the membranes used for adhering of bacterial cells onto them?
A.	Nitrocellulose membranes were preferred earlier
B.	They bind DNA very efficiently
C.	They can be handled easily without breakage
D.	They are inflammable
Answer» D. They are inflammable

4.	The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps:
A.	Peeling of membranes carrying away bacterial cells with it
B.	Cells are lysed and denaturing of DNA is being carried out
C.	Hybridization with labelled DNA
D.	Placing the membrane onto plate containing recombinant cells
Answer» B. Cells are lysed and denaturing of DNA is being carried out

5.	Choose the incorrect statement for colony or plaque lift.
A.	It is the base for screening based on nucleic acid hybridization
B.	It is also known as Grunstein-Hogness technique
C.	It is based on the fact that which members of the library have the same sequence as the DNA probe
D.	The library may be plaques on a bacterial lawn and in that case it is known as plaque lift
Answer» D. The library may be plaques on a bacterial lawn and in that case it is known as plaque lift

6.	How many techniques are there for carrying out the screening of sequences encoding for RNAs?
A.	1
B.	2
C.	3
D.	4
Answer» C. 3

7.	If screening is carried out on the basis of sequences which are related to the desired sequence, then the process is called as:
A.	in-silico
B.	homologue search
C.	annotation
D.	partial search
Answer» D. partial search

8.	If we are having sequence data for a particular organism, but screening is carried out for homologues the program used is BLAST. Is the given statement true or false?
A.	True
B.	False
Answer» B. False

9.	If for a particular organism sequence data is available and we have to simply search in the data through computer, then this method is called as:
A.	annotation
B.	database search
C.	in silico
D.	electronic search
Answer» D. electronic search

10.	The process of finding a particular member of library which is having some defined properties is called as:
A.	searching
B.	screening
C.	locating
D.	narrowing
Answer» C. locating