Choose the incorrect statement with respect to the membranes used for adhering of bacterial cells onto them.

Nitrocellulose membranes were preferred earlier

They can be handled easily without breakage

Choose the incorrect statement for colony or plaque lift.

The library may be plaques on a bacterial lawn and in that case it is known as plaque lift

It is the base for screening based on nucleic acid hybridization

It is also known as Grunstein-Hogness technique

It is based on the fact that which members of the library have the same sequence as the DNA probe

The library may be plaques on a bacterial lawn and in that case it is known as plaque lift

1.	What is used for lysing of bacterial cells and denaturation of DNA?
A.	Exonuclease
B.	Sulphuric Acid
C.	Sodium Hydroxide
D.	Heat
Answer» D. Heat

2.	Nitrocellulose membranes are less sensitive than nylon membranes.
A.	True
B.	False
Answer» C.

3.	Choose the incorrect statement with respect to the membranes used for adhering of bacterial cells onto them.
A.	Nitrocellulose membranes were preferred earlier
B.	They bind DNA very efficiently
C.	They can be handled easily without breakage
D.	They are inflammable
Answer» D. They are inflammable

4.	The screening of libraries us carried out by nucleic acid hybridisation and constitutes of following steps:i) Peeling of membranes carrying away bacterial cells with itii) Cells are lysed and denaturing of DNA is being carried outiii) Hybridization with labelled DNAiv) Placing the membrane onto plate containing recombinant cellsChoose the correct sequence in which the steps are carried out (starting to ending).
A.	iv)-i)-ii)-iii)
B.	iv)-ii)-i)-iii)
C.	i)-ii)-iii)-iv)
D.	iii)-ii)-i)-iv)
Answer» B. iv)-ii)-i)-iii)

5.	Choose the incorrect statement for colony or plaque lift.
A.	It is the base for screening based on nucleic acid hybridization
B.	It is also known as Grunstein-Hogness technique
C.	It is based on the fact that which members of the library have the same sequence as the DNA probe
D.	The library may be plaques on a bacterial lawn and in that case it is known as plaque lift
Answer» D. The library may be plaques on a bacterial lawn and in that case it is known as plaque lift

6.	How many techniques are there for carrying out the screening of sequences encoding for RNAs?
A.	1
B.	2
C.	3
D.	4
Answer» C. 3

7.	If screening is carried out on the basis of sequences which are related to the desired sequence, then the process is called ___________
A.	in-silico
B.	homologue search
C.	annotation
D.	partial search
Answer» D. partial search

8.	If we are having sequence data for a particular organism, but screening is carried out for homologues the program used is BLAST.
A.	True
B.	False
Answer» B. False

9.	If for a particular organism sequence data is available and we have to simply search in the data through a computer, then this method is called as ___________
A.	annotation
B.	database search
C.	in silico
D.	electronic search
Answer» D. electronic search

10.	The process of finding a particular member of the library which is having some defined properties is called as ___________
A.	searching
B.	screening
C.	locating
D.	narrowing
Answer» C. locating