 
			 
			MCQOPTIONS
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				This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Cell Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | The process of dedifferentiation in cell culture can give rise to ________________________ | 
| A. | induced-pluripotent stem cells | 
| B. | carcinoma cells | 
| C. | single protoplasts | 
| D. | fused protoplasts | 
| Answer» B. carcinoma cells | |
| 2. | Under favorable conditions, the protoplasts can grow into a ____________________ | 
| A. | callus culture | 
| B. | organ culture | 
| C. | root culture | 
| D. | shoot culture | 
| Answer» B. organ culture | |
| 3. | HeLa cells are a cell line. | 
| A. | True | 
| B. | False | 
| Answer» B. False | |
| 4. | EDTA binds the ______________ ions. | 
| A. | magnesium | 
| B. | iron | 
| C. | carbon | 
| D. | calcium | 
| Answer» E. | |
| 5. | In the secondary culture, cells are obtained from _______________________ | 
| A. | primary culture | 
| B. | the organism | 
| C. | organ culture | 
| D. | phenotypic culture | 
| Answer» B. the organism | |
| 6. | Trypsin is used for dissociating the tissue into single cells. | 
| A. | True | 
| B. | False | 
| Answer» B. False | |
| 7. | Silver thiosulphate is added to culture medium as it helps to | 
| A. | maintain the pH | 
| B. | remove toxic phenolics from plant cells | 
| C. | prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition. | 
| D. | all of the above | 
| Answer» D. all of the above | |
| 8. | What is 'nurse' or conditioned medium? | 
| A. | It is the media full of growth factors used for the growth of cells | 
| B. | It is the medium added to nurse the callus culture | 
| C. | Both (a) and (b) | 
| D. | It is the liquid medium removed from the suspension of fast growing cells | 
| Answer» E. | |
| 9. | Which of the following is not true about nurse or conditioned medium? | 
| A. | It is liquid removed from the suspension of fast growing cells | 
| B. | It contains uncharacterized growth factor released by growing cells | 
| C. | It is used in the culture of regenerating protoplast | 
| D. | it is removed aseptically from the culture and is autoclaved before use | 
| Answer» E. | |
| 10. | To maintain the pH of the culture | 
| A. | organic acid such as citric, fumaric, malic and succinic acid is used | 
| B. | synthetic buffers such as Tris, MES or HEPS are used | 
| C. | both (a) and (b) | 
| D. | ammonium salts are used | 
| Answer» D. ammonium salts are used | |
| 11. | Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media | 
| A. | utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise | 
| B. | utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise | 
| C. | utilization of both ammonium and nitrate result in rise in pH | 
| D. | utilization of both ammonium and nitrate result in drop in pH | 
| Answer» B. utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise | |
| 12. | Which of the following growth regulator is used to stimulate embryo or shoot development? | 
| A. | Auxins | 
| B. | Cytokinins | 
| C. | Gibberellins | 
| D. | Brassinosteroids | 
| Answer» D. Brassinosteroids | |
| 13. | Which of the following growth regulator is added for short initiation during plant regeneration from callus? | 
| A. | Auxins | 
| B. | Cytokinins | 
| C. | Gibberellins | 
| D. | Brassinosteroids | 
| Answer» C. Gibberellins | |
| 14. | Which of the following growth regulator promote cell division? | 
| A. | Auxins | 
| B. | Cytokinins | 
| C. | Gibberellins | 
| D. | Brassinosteroids | 
| Answer» C. Gibberellins | |
| 15. | Which is the most common carbon source used in the plant cell culture media? | 
| A. | Sucrose | 
| B. | Glucose | 
| C. | Fructose | 
| D. | Maltose | 
| Answer» B. Glucose | |