MCQOPTIONS
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This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Cell Biology knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The process of dedifferentiation in cell culture can give rise to ________________________ |
| A. | induced-pluripotent stem cells |
| B. | carcinoma cells |
| C. | single protoplasts |
| D. | fused protoplasts |
| Answer» B. carcinoma cells | |
| 2. |
Under favorable conditions, the protoplasts can grow into a ____________________ |
| A. | callus culture |
| B. | organ culture |
| C. | root culture |
| D. | shoot culture |
| Answer» B. organ culture | |
| 3. |
HeLa cells are a cell line. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 4. |
EDTA binds the ______________ ions. |
| A. | magnesium |
| B. | iron |
| C. | carbon |
| D. | calcium |
| Answer» E. | |
| 5. |
In the secondary culture, cells are obtained from _______________________ |
| A. | primary culture |
| B. | the organism |
| C. | organ culture |
| D. | phenotypic culture |
| Answer» B. the organism | |
| 6. |
Trypsin is used for dissociating the tissue into single cells. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 7. |
Silver thiosulphate is added to culture medium as it helps to |
| A. | maintain the pH |
| B. | remove toxic phenolics from plant cells |
| C. | prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition. |
| D. | all of the above |
| Answer» D. all of the above | |
| 8. |
What is 'nurse' or conditioned medium? |
| A. | It is the media full of growth factors used for the growth of cells |
| B. | It is the medium added to nurse the callus culture |
| C. | Both (a) and (b) |
| D. | It is the liquid medium removed from the suspension of fast growing cells |
| Answer» E. | |
| 9. |
Which of the following is not true about nurse or conditioned medium? |
| A. | It is liquid removed from the suspension of fast growing cells |
| B. | It contains uncharacterized growth factor released by growing cells |
| C. | It is used in the culture of regenerating protoplast |
| D. | it is removed aseptically from the culture and is autoclaved before use |
| Answer» E. | |
| 10. |
To maintain the pH of the culture |
| A. | organic acid such as citric, fumaric, malic and succinic acid is used |
| B. | synthetic buffers such as Tris, MES or HEPS are used |
| C. | both (a) and (b) |
| D. | ammonium salts are used |
| Answer» D. ammonium salts are used | |
| 11. |
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media |
| A. | utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise |
| B. | utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise |
| C. | utilization of both ammonium and nitrate result in rise in pH |
| D. | utilization of both ammonium and nitrate result in drop in pH |
| Answer» B. utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise | |
| 12. |
Which of the following growth regulator is used to stimulate embryo or shoot development? |
| A. | Auxins |
| B. | Cytokinins |
| C. | Gibberellins |
| D. | Brassinosteroids |
| Answer» D. Brassinosteroids | |
| 13. |
Which of the following growth regulator is added for short initiation during plant regeneration from callus? |
| A. | Auxins |
| B. | Cytokinins |
| C. | Gibberellins |
| D. | Brassinosteroids |
| Answer» C. Gibberellins | |
| 14. |
Which of the following growth regulator promote cell division? |
| A. | Auxins |
| B. | Cytokinins |
| C. | Gibberellins |
| D. | Brassinosteroids |
| Answer» C. Gibberellins | |
| 15. |
Which is the most common carbon source used in the plant cell culture media? |
| A. | Sucrose |
| B. | Glucose |
| C. | Fructose |
| D. | Maltose |
| Answer» B. Glucose | |