MCQOPTIONS
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MCQOPTIONS
This section includes 10 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
For the synthesis of the second DNA strand, incorporated oligo-dT is of no use. Why? |
| A. | It is so because it is unstable |
| B. | It is so because it is at the 3’ end of the template molecule |
| C. | It is so because it would be unable to initiate replication |
| D. | It is so because it detaches itself after first strand synthesis |
| Answer» D. It is so because it detaches itself after first strand synthesis | |
| 2. |
The first step for RNaseH method is to anneal a chemically synthesized oligo-dT primer to the 3’ polyA tail of RNA. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 3. |
What is the basis of RNaseH method? |
| A. | It is based on RNA synthesis by DNA strand |
| B. | It is based on complementary DNA synthesis by RNA strand through reverse transcriptase |
| C. | It is based on complementary DNA synthesis by RNA strand through RNaseH enzyme |
| D. | It is based on getting double strand RNA from a single strand |
| Answer» C. It is based on complementary DNA synthesis by RNA strand through RNaseH enzyme | |
| 4. |
Choose the correct with respect to RNA molecules. |
| A. | They are less labile than DNA molecules |
| B. | The 2’ hydroxyl group of ribose group decreases the activity |
| C. | There is no less of activity while boiling |
| D. | Baking of glassware, treatment with UV can be used for protection against degradation |
| Answer» E. | |
| 5. |
At times streptavidin is used in place of cellulose. Choose the correct statement for this alternative. |
| A. | oligo-dT streptavidin conjugate can be extracted by magnetic beads |
| B. | The magnetic beads are attached with activin |
| C. | Recovery is not based on the magnetic properties of the beads |
| D. | The interaction between polyA tail and the oligo-dT is reduced |
| Answer» B. The magnetic beads are attached with activin | |
| 6. |
Poly A tail from the column is eluted by using high salt concentration. |
| A. | True |
| B. | False |
| Answer» C. | |
| 7. |
Choose the incorrect statement for oligo-dT cellulose. |
| A. | It is used for separation of polyadenylated mRNA from another mRNA |
| B. | oligo-dT are covalently attached to the solid support via OH bonds |
| C. | A solution containing RNA is passed through the column |
| D. | Poly A tail attaches to the oligo-dT by ionic bonds |
| Answer» E. | |
| 8. |
Polyadenylation of RNA species is an important criterion for the production of cDNA species. Which of the following holds true? |
| A. | Polyadenylation should be at 3’ end |
| B. | Eukaryotic mRNAs are mostly non-polyadenylated |
| C. | Bacterial mRNAs and organelle mRNAs are polyadenylated |
| D. | It is carried out by the addition of T residues after synthesis |
| Answer» B. Eukaryotic mRNAs are mostly non-polyadenylated | |
| 9. |
A times partial sequencing of cloned cDNAs is carried out. These cDNA are known as ___________ |
| A. | expressed RNA sequences |
| B. | expressed sequence tags (ESTs) |
| C. | expressed cDNA sequences |
| D. | library |
| Answer» C. expressed cDNA sequences | |
| 10. |
Choose the incorrect statement for cDNA libraries. |
| A. | They constitute of DNA copies produced from the RNA sequences and usually mRNA |
| B. | They represent expressed sequences |
| C. | Introns are not represented |
| D. | Comparison of cDNA sequences with genomic sequences leads to the determination of polyadenylation sites |
| Answer» D. Comparison of cDNA sequences with genomic sequences leads to the determination of polyadenylation sites | |