For the synthesis of the second DNA strand, incorporated oligo-dT is of no use. Why?

It is so because it detaches itself after first strand synthesis

It is so because it is unstable

It is so because it is at the 3 end of the template molecule

It is so because it would be unable to initiate replication

It is so because it detaches itself after first strand synthesis

Explore topic-wise MCQs in Genetic Engineering.

This section includes 4 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.	For the synthesis of the second DNA strand, incorporated oligo-dT is of no use. Why?
A.	It is so because it is unstable
B.	It is so because it is at the 3 end of the template molecule
C.	It is so because it would be unable to initiate replication
D.	It is so because it detaches itself after first strand synthesis
Answer» D. It is so because it detaches itself after first strand synthesis

Discussion

2.	The first step for RNaseH method is to anneal a chemically synthesized oligo-dT primer to the 3 polyA tail of RNA.
A.	True
B.	False
Answer» B. False

Discussion

3.	Poly A tail from the column is eluted by using high salt concentration.
A.	True
B.	False
Answer» C.

Discussion

4.	A times partial sequencing of cloned cDNAs is carried out. These cDNA are known as ___________
A.	expressed RNA sequences
B.	expressed sequence tags (ESTs)
C.	expressed cDNA sequences
D.	library
Answer» C. expressed cDNA sequences

Discussion

Explore topic-wise MCQs in Genetic Engineering.

For the synthesis of the second DNA strand, incorporated oligo-dT is of no use. Why?

The first step for RNaseH method is to anneal a chemically synthesized oligo-dT primer to the 3 polyA tail of RNA.

Poly A tail from the column is eluted by using high salt concentration.

A times partial sequencing of cloned cDNAs is carried out. These cDNA are known as ___________