What are the effects of increasing concentration of reaction components?

It decreases chances of ligation in intramolecular and increases in that of intermolecular

It increases chances of ligation in both intramolecular and intermolecular reactions

It increases chances of ligation only in intermolecular and no effect on intramolecular

It decreases chances of ligation in intramolecular and increases in that of intermolecular

It decreases chances of ligation in both types of reaction

What is the kinetics of the intramolecular and intermolecular ligation reactions?

Second order kinetics for intramolecular and first order for intermolecular

First order kinetics for intramolecular and second order for intermolecular

In the case of blunt-end ligation, blunt ends can be generated by ___________

by the action of restriction endonuclease which gives staggered ends

simply the action of restriction endonuclease which gives straight ends

the polishing of staggered ends

both the action of restriction endonuclease which gives straight ends and polishing of staggered ends

by the action of restriction endonuclease which gives staggered ends

Ligation is defined as ______________

Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands

Alignment of only double stranded DNA molecules at the ends and the formation of phosphodiester bonds between both the strands

Alignment of either of the double or single stranded DNA molecules and formation of glycosidic bonds between both the strands

Alignment of either of the double or single stranded DNA molecules and the formation of phosphodiester bonds. The bond can be between either one or both the strands

Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands

Explore topic-wise MCQs in Genetic Engineering.

This section includes 6 Mcqs, each offering curated multiple-choice questions to sharpen your Genetic Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.	What are the effects of increasing concentration of reaction components?
A.	It increases chances of ligation in both intramolecular and intermolecular reactions
B.	It increases chances of ligation only in intermolecular and no effect on intramolecular
C.	It decreases chances of ligation in intramolecular and increases in that of intermolecular
D.	It decreases chances of ligation in both types of reaction
Answer» C. It decreases chances of ligation in intramolecular and increases in that of intermolecular

Discussion

2.	What is the kinetics of the intramolecular and intermolecular ligation reactions?
A.	Second order kinetics for intramolecular and first order for intermolecular
B.	First order kinetics for intramolecular and second order for intermolecular
C.	Both are first order
D.	Both are second order
Answer» C. Both are first order

Discussion

3.	Ligation reaction can be both intramolecular and intermolecular in nature.
A.	True
B.	False
Answer» B. False

Discussion

4.	In the case of blunt-end ligation, blunt ends can be generated by ___________
A.	simply the action of restriction endonuclease which gives straight ends
B.	the polishing of staggered ends
C.	both the action of restriction endonuclease which gives straight ends and polishing of staggered ends
D.	by the action of restriction endonuclease which gives staggered ends
Answer» D. by the action of restriction endonuclease which gives staggered ends

Discussion

5.	If only one bond is broken in the sugar-phosphate backbone, it is called as ___________
A.	gap
B.	nick
C.	break
D.	leakage
Answer» C. break

Discussion

6.	Ligation is defined as ______________
A.	Alignment of only double stranded DNA molecules at the ends and the formation of phosphodiester bonds between both the strands
B.	Alignment of either of the double or single stranded DNA molecules and formation of glycosidic bonds between both the strands
C.	Alignment of either of the double or single stranded DNA molecules and the formation of phosphodiester bonds. The bond can be between either one or both the strands
D.	Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands
Answer» D. Alignment of single stranded DNA molecules and formation of glycosidic bonds between these strands

Discussion

Explore topic-wise MCQs in Genetic Engineering.

What are the effects of increasing concentration of reaction components?

What is the kinetics of the intramolecular and intermolecular ligation reactions?

Ligation reaction can be both intramolecular and intermolecular in nature.

In the case of blunt-end ligation, blunt ends can be generated by ___________

If only one bond is broken in the sugar-phosphate backbone, it is called as ___________

Ligation is defined as ______________