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This section includes 94 Mcqs, each offering curated multiple-choice questions to sharpen your SRMJEEE knowledge and support exam preparation. Choose a topic below to get started.
1. |
DNA fragments separated on an agarose gel can be visualized after staining with ____ |
A. | ethidium bromide |
B. | bromophenol blue |
C. | acetocarmine |
D. | aniline blue |
Answer» B. bromophenol blue | |
2. |
Animal biotechnology involves |
A. | production of valuable products in animals using rDNA technology |
B. | rapid multiplication of animals of desired genotypes |
C. | alteration of genes to make it more desirable |
D. | all of these |
Answer» E. | |
3. |
A gene whose expression helps to identify transformed cells is known as ____ |
A. | Plasmid |
B. | Selectable marker |
C. | Structural gene |
D. | vector |
Answer» C. Structural gene | |
4. |
In humans, the babies produced by in vitro fertilization and embryo transfer was popularly called as |
A. | invitro babies |
B. | test tube babies |
C. | invitro-invivo babies |
D. | all of these |
Answer» C. invitro-invivo babies | |
5. |
Which of the following is the quality of improved transgenic basmati rice? |
A. | Gives high yield but no characteristic aroma |
B. | Gives high yield and is rich in vitamin A |
C. | Does not require chemical fertilizers and growth hormones |
D. | Resistant to insects and diseases |
Answer» C. Does not require chemical fertilizers and growth hormones | |
6. |
PCR technique was invented by |
A. | Karry Mullis |
B. | Boyer |
C. | Sanger |
D. | Cohn |
Answer» B. Boyer | |
7. |
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form arecombinant plasmid using |
A. | Taq polymerase |
B. | Polymerase III |
C. | Ligase |
D. | Eco RI |
Answer» D. Eco RI | |
8. |
Hybrid antibodies are |
A. | antibodies produced in cell cultures |
B. | antibodies designed using rDNA technology produced in cell cultures |
C. | antibodies produced in invivo |
D. | both a and b |
Answer» C. antibodies produced in invivo | |
9. |
The first vaccine developed from animal cell culture was |
A. | Hepatitis B vaccine |
B. | Influenza vaccine |
C. | Small pox vaccine |
D. | Polio vaccine |
Answer» E. | |
10. |
Klenow fragment is derived from |
A. | DNA Ligase |
B. | DNA Pol-I |
C. | DNA Pol-II |
D. | Reverse Transcriptase |
Answer» C. DNA Pol-II | |
11. |
The Taq polymerase enzyme is obtained from |
A. | Thermus aquaticus |
B. | Thiobacillus ferrooxidans |
C. | Bacillus subtilis |
D. | Pseudomonas subtilis |
Answer» B. Thiobacillus ferrooxidans | |
12. |
The virus commonly used to infect cell cultures for the production of interferon is |
A. | Corona virus |
B. | Sendai virus |
C. | Polio virus |
D. | Small pox virus |
Answer» C. Polio virus | |
13. |
The production of complete animals from somatic cells of an animal is called |
A. | gene cloning |
B. | animal cloning |
C. | cell cloning |
D. | all of these |
Answer» C. cell cloning | |
14. |
The DNA fragments have sticky ends due to |
A. | Endonuclease |
B. | Unpaired bases |
C. | Calcium ions |
D. | Free methylation |
Answer» C. Calcium ions | |
15. |
Southern blotting is |
A. | Attachment of probes to DNA fragments |
B. | Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet |
C. | Comparison of DNA fragments to two sources |
D. | Transfer of DNA fragments to electrophoretic gel from cellulose membrane |
Answer» C. Comparison of DNA fragments to two sources | |
16. |
Excision and insertion of a gene is called |
A. | Biotechnology |
B. | Genetic engineering |
C. | Cytogenetics |
D. | Gene therapy |
Answer» C. Cytogenetics | |
17. |
Recombinant proteins are |
A. | proteins synthesized in animals |
B. | proteins synthesized by transgene in host cell by rDNA technology |
C. | proteins synthesised in cells that are produced by protoplast fusion |
D. | proteins synthesized in mutated cell lines |
Answer» C. proteins synthesised in cells that are produced by protoplast fusion | |
18. |
RNA interference helps in |
A. | Cell proliferation |
B. | Micropropagation |
C. | Cell defence |
D. | Cell differentiation |
Answer» D. Cell differentiation | |
19. |
Agarose extracted from sea weeds finds use in ____ |
A. | Spectrophotometry |
B. | Gel electrophoresis |
C. | PCR |
D. | tissue culture |
Answer» C. PCR | |
20. |
The first successfully cloned animal was |
A. | monkey |
B. | gibbon |
C. | sheep |
D. | rabbit |
Answer» D. rabbit | |
21. |
Restriction enzymes were discovered by |
A. | Smith and Nathans |
B. | Alexander Fleming |
C. | Berg |
D. | None |
Answer» B. Alexander Fleming | |
22. |
Which of the following is an endonuclease? |
A. | DNase I |
B. | Hind II |
C. | Protease |
D. | RNase restriction |
Answer» C. Protease | |
23. |
A single strand of nucleic acid tagged with a radioactive molecule is called |
A. | Plasmid |
B. | Probe |
C. | selectable marker |
D. | Vector |
Answer» C. selectable marker | |
24. |
Bacteria protect themselves from viruses by fragmenting viral DNA with |
A. | Ligase |
B. | Endonuclease |
C. | Exonuclease |
D. | Gyrase |
Answer» C. Exonuclease | |
25. |
The technique used in animal biotechnology for the rapid multiplication and production ofanimals with a desirable genotype is |
A. | protoplast fusion and embryo transfer |
B. | hybrid selection and embryo transfer |
C. | in vitro fertilization and embryo transfer |
D. | all of these |
Answer» D. all of these | |
26. |
The first transgenic plant to be produced is |
A. | Brinjal |
B. | Tobacco |
C. | Rice |
D. | Cotton |
Answer» C. Rice | |
27. |
The human genome project was launched in the year |
A. | 1980 |
B. | 1973 |
C. | 1990 |
D. | 1989 |
Answer» D. 1989 | |
28. |
Interferons are |
A. | anti bacterial proteins |
B. | anti-viral proteins |
C. | bacteriostatic proteins |
D. | all of these |
Answer» C. bacteriostatic proteins | |
29. |
The cell line used for the production of polio vaccine was |
A. | Primate kidney cell line |
B. | CHO cell line |
C. | Dog kidney cell line |
D. | mouse fibroblast cell line |
Answer» B. CHO cell line | |
30. |
The vaccines prepared through recombinant DNA technology are |
A. | Third generation vaccines |
B. | First-generation vaccines |
C. | Second-generation vaccines |
D. | None |
Answer» B. First-generation vaccines | |
31. |
Which of the following are commonly produced in animal cell cultures |
A. | Interferon |
B. | mab |
C. | vaccines |
D. | all of these |
Answer» E. | |
32. |
There is a restriction endonuclease called Eco RI. What does ‘co’ part in it stand for? |
A. | Coli |
B. | Colon |
C. | Cofactor |
D. | None of the above |
Answer» B. Colon | |
33. |
Which of the following restriction enzymes produce blunt ends? |
A. | Hind III |
B. | ho |
C. | Eco RV |
D. | Sal I |
Answer» D. Sal I | |
34. |
Animal cell cultures are used widely for the production of |
A. | insulin |
B. | somatostatin |
C. | mabs |
D. | thyroxine |
Answer» D. thyroxine | |
35. |
ELISA is |
A. | Using radiolabelled second antibody |
B. | Usage of RBCs |
C. | Using complement-mediated cell lysis |
D. | Addition of substrate that is converted into a coloured end product |
Answer» E. | |
36. |
The first clinical application of gene therapy over a 4 year old girl was for |
A. | Adenosine deaminase deficiency |
B. | Adenosine deficiency |
C. | Growth deficiency |
D. | Adenine deficiency |
Answer» B. Adenosine deficiency | |
37. |
Which of the following is not a component of downstream processing? |
A. | Expression |
B. | Preservation |
C. | Purification |
D. | Separation |
Answer» B. Preservation | |
38. |
Which is a genetically modified crop? |
A. | Bt-cotton |
B. | Bt-brinjal |
C. | Golden rice |
D. | All |
Answer» E. | |
39. |
The expression of a transgene in the target tissue is identified by a |
A. | Transgene |
B. | Promoter |
C. | Enhancer |
D. | Reporter |
Answer» E. | |
40. |
The Golden Rice variety is rich in |
A. | Vitamin C |
B. | Β-carotene and ferritin |
C. | Biotin |
D. | Lysine |
Answer» C. Biotin | |
41. |
Plasmids are used as cloning vectors for which of the following reasons? |
A. | Can be multiplied in culture |
B. | Self-replication in bacterial cells |
C. | Can be multiplied in laboratories with the help of enzymes |
D. | Replicate freely outside bacterial cells |
Answer» C. Can be multiplied in laboratories with the help of enzymes | |
42. |
Which bacterium is used in the production of insulin by genetic engineering? |
A. | Saccharomyces |
B. | Rhizobium |
C. | Escherichia |
D. | Mycobacterium |
Answer» D. Mycobacterium | |
43. |
———– is used as a vector for cloning into higher organisms |
A. | Retrovirus |
B. | Baculovirus |
C. | Salmonella typhimurium |
D. | Rhizopus nigricans |
Answer» B. Baculovirus | |
44. |
A cross shaped (+) or a ring involving four chromosomes may be formed during metaphase I in case of |
A. | translocation heterozygote |
B. | deficiency |
C. | haploid organism |
D. | duplication |
Answer» B. deficiency | |
45. |
The linking of antibiotic resistance gene with the plasmid vector became possible with: |
A. | Endonucleases |
B. | DNA ligase |
C. | Exonucleases |
D. | DNA polymerase |
Answer» C. Exonucleases | |
46. |
For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of |
A. | Silicon or Platinum |
B. | Gold or Tungsten |
C. | Silver or Platinum |
D. | Platinum or Zinc |
Answer» C. Silver or Platinum | |
47. |
Which part would be most suitable for raising virus-free plants for micropropagation? |
A. | Meristem |
B. | Node |
C. | Bark |
D. | Vascular tissue |
Answer» B. Node | |
48. |
Which one of the following is a case of wrong matching? |
A. | Micropropagation-In vitro production of plants in large numbers |
B. | Callus - Unorganized mass of cells produced in tissue culture |
C. | Somatic hybridization - Fusion of two diverse cells |
D. | Vector DNA - Site for t-RNA synthesis |
Answer» E. | |
49. |
Which one is a true statement regarding DNA polymerase used in PCR? |
A. | It is isolated from a virus |
B. | It remains active at high temperature |
C. | It is used to ligate introduced DNA in recipient cells |
D. | It serves as a selectable marker |
Answer» C. It is used to ligate introduced DNA in recipient cells | |
50. |
Which one is found only in RNA? |
A. | Cytosine |
B. | Adenine |
C. | Uracil |
D. | Guanine |
Answer» D. Guanine | |