MCQOPTIONS
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MCQOPTIONS
This section includes 26 Mcqs, each offering curated multiple-choice questions to sharpen your Biophysical knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The molar extinction coefficients of Trp and Tyr at 280 nm are 5690 and 1280 M 1.cm 1, respectively. The polypeptide chain of yeast alcohol dehydrogenase (37 kDa) contains 5 Trp and 14 Tyr residues. The absorbance at 280 nm of a 0.32 mg.mL 1 solution of yeast alcohol dehydrogenase measured in a cuvette of 1 cm pathlength will be _________. (Assume that the molar extinction coefficient values for Trp and Tyr apply to these amino acids in the yeast alcohol dehydrogenase). |
| A. | 0.37 to 0.43 |
| B. | 0.37 |
| C. | 0.23 |
| D. | None of the above |
| Answer» B. 0.37 | |
| 2. |
Match the entries in the Group I with the elution conditions in Group II. Group I P. Ion-exchange Q. Hydrophobic chromatography R. Gel filtration column chromatography S. Chromatofocusing Group II 1. Isocratic solvent chromatography 2. Ampholytes 3. Increasing gradient of salt 4. Decreasing gradient of polarity |
| A. | P-4,Q-1,R-2,S-3 |
| B. | P-4,Q-3,R-1,S-2 |
| C. | P-3,Q-4,R-1,S-2 |
| D. | P-3,Q-4,R-2,S-1 |
| Answer» D. P-3,Q-4,R-2,S-1 | |
| 3. |
A protein is to be purified using ion- exchange column chromatography. The relationship between HETP (Height Equivalent to Theoretical Plate) and the linear liquid velocity of mobile phase is given by :H = A + Bu + Cuwhere H is HETP (m) and u is linear liquid velocity of mobile phase (m.s 1). The values of A, B and C are 3 10 8 m2.s 1, 3 s and 6 10 5 m, respectively. The number of theoretical plates based on minimum HETP for a column of 66 cm length will be ___________. |
| A. | 2000 |
| B. | 500 |
| C. | 1000 |
| D. | All of the above |
| Answer» D. All of the above | |
| 4. |
Match each parameter in group 1 with the appropriate measuring device in group 2 Group 1 P. Pressure Q. Foam R. Turbidity S. Flow rate Group 2 1. Photometer 2. Rotameter 3. Diaphragm gauge 4. Rubber sheathed electrode |
| A. | P-3, Q-4, R-l, S-2 |
| B. | P-l, Q-3, R-2, S-4 |
| C. | P-4, Q-l, R-2, S-3 |
| D. | P-l, Q-2, R-3, S-4 |
| Answer» B. P-l, Q-3, R-2, S-4 | |
| 5. |
Match the items in Group I with Group II. Group I P. Circular dichroism Q. X-ray crystallography R. Freeze-drying S. Ultracentrifugation Group II 1. Concentration 2. Sedimentation coefficient 3. Secondary structure determination 4. Tertiary structure determination |
| A. | P - 4 , Q - 1 , R - 2 , S - 3 |
| B. | P - 1 , Q - 4 , R - 3 , S - 2 |
| C. | P - 2 , Q - 3 , R - 4 , S - 1 |
| D. | P - 3 , Q - 4 , R - 1 , S - 2 |
| Answer» E. | |
| 6. |
Match the entries in Group I with the methods of sterilization in Group II. Group I P. Serum Q. Luria broth f ltration R. Polypropylene tubes S. Biological safety cabinets Group II 1. Autoclave 2. Membrane 3. UV irradiation 4. Gamma irradiation 5. Dry heat |
| A. | P - 5 , Q - 3 , R - 1 , S - 4 |
| B. | P - 1 , Q - 4 , R - 5 , S - 3 |
| C. | P - 2 , Q - 1 , R - 4 , S - 3 |
| D. | P - 4 , Q - 1 , R - 3 , S - 5 |
| Answer» D. P - 4 , Q - 1 , R - 3 , S - 5 | |
| 7. |
Match the coefficients in group 1 with their corresponding downstream processing steps given in group 2 Group 1 P. Sedimentation coefficient Q. Partition coefficient R. Rejection coefficient S. Activity coefficient Group 2 1. Aqueous two phase extraction 2. Ultrafiltration 3. Dialysis 4. Centrifugation |
| A. | P-3 ,Q-1 ,R-4 , S-2 |
| B. | P-2 ,Q-1 ,R-4 , S-3 |
| C. | P-4 ,Q-3 ,R-1 , S-2 |
| D. | P-4 ,Q-1 ,R-2 , S-3 |
| Answer» E. | |
| 8. |
Match the properties in Group 1 with the downstream operation in Group 2 : Group 1 Group 2 P. Size 1. Extraction Q. Density 2. Distillation R. Volatility 3. Filtration S. Solubility 4. Sedimentation |
| A. | P-3, Q-4, R-2, S-1 |
| B. | P-4, Q-1, R-2, S-3 |
| C. | P-4, Q-3, R-1, S-2 |
| D. | P-3, Q-2, R-4, S-1 |
| Answer» B. P-4, Q-1, R-2, S-3 | |
| 9. |
Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, , of a double stranded Cs+ DNA is given by the equation = 1.66 + 0.098XG + C where XG + C denotes |
| A. | total number of G and C |
| B. | mole fraction of G + C |
| C. | number of GC repeats |
| D. | ratio of G + C to A + T content |
| Answer» C. number of GC repeats | |
| 10. |
When electroporation is used for introducing DNA into mammalian cells (P) a carrier for DNA is not required (Q) the lipid bilayer (membrane) interacts with an electric pulse to generate permeation sites (R) the viability of the cells becomes approximately zero percent (S) the first step involves absorption of DNA on the cell membrane |
| A. | P, Q |
| B. | Q, R |
| C. | P, S |
| D. | Q, S |
| Answer» B. Q, R | |
| 11. |
Mechanism of separation of contaminants present in air by fibrous media are |
| A. | Interception |
| B. | Inertial impaction |
| C. | Diffusion |
| D. | All of these |
| Answer» E. | |
| 12. |
Cibacren Blue dye affinity chromatography can be used for affinity purification of |
| A. | NADPH dehydrogenase |
| B. | glucoamylase |
| C. | subtilisin |
| D. | caspase |
| Answer» B. glucoamylase | |
| 13. |
Ultrafiltration process can not be used for |
| A. | fractionation of proteins |
| B. | desalting |
| C. | harvesting of cells |
| D. | selective removal of solvents |
| Answer» D. selective removal of solvents | |
| 14. |
When electroporation is used for introducing DNA into mammalian cells |
| A. | P, Q |
| B. | Q, R |
| C. | P, S |
| D. | Q, S |
| Answer» B. Q, R | |
| 15. |
The molar extinction coefficients of Trp and Tyr at 280 nm are 5690 and 1280 M |
| A. | 0.37 to 0.43 |
| B. | 0.37 |
| C. | 0.23 |
| D. | None of the above |
| Answer» B. 0.37 | |
| 16. |
In transmission electron microscopy, electron opacity is greatly enhanced by treating the specimen with |
| A. | ferrous ammonium sulfate |
| B. | uranium acetate |
| C. | sodium chloride |
| D. | basic fuchsin |
| Answer» C. sodium chloride | |
| 17. |
In dead-end filtration, rate of filtration is |
| A. | directly proportional to the square root of pressure drop across the filter medium |
| B. | inversely proportional to the pressure drop across the filter medium |
| C. | inversely proportional to the viscosity of the solution |
| D. | inversely proportional to the square of viscosity of the solution |
| Answer» D. inversely proportional to the square of viscosity of the solution | |
| 18. |
Which one of the following techniques relies on the spin angular momentum of a photon? |
| A. | CD spectroscopy |
| B. | Fluorescence spectroscopy |
| C. | IR spectroscopy |
| D. | Raman spectroscopy |
| Answer» B. Fluorescence spectroscopy | |
| 19. |
Cesium chloride density gradient centrifugation is commonly used for the separation of DNA molecules. The buoyant density, , of a double stranded Cs |
| A. | total number of G and C |
| B. | mole fraction of G + C |
| C. | number of GC repeats |
| D. | ratio of G + C to A + T content |
| Answer» C. number of GC repeats | |
| 20. |
Match the entries in the Group I with the elution conditions in Group II. |
| A. | P-4,Q-1,R-2,S-3 |
| B. | P-4,Q-3,R-1,S-2 |
| C. | P-3,Q-4,R-1,S-2 |
| D. | P-3,Q-4,R-2,S-1 |
| Answer» D. P-3,Q-4,R-2,S-1 | |
| 21. |
A protein is to be purified using ion- exchange column chromatography. The relationship between HETP (Height Equivalent to Theoretical Plate) and the linear liquid velocity of mobile phase is given by : |
| A. | 2000 |
| B. | 500 |
| C. | 1000 |
| D. | All of the above |
| Answer» D. All of the above | |
| 22. |
Match the coefficients in group 1 with their corresponding downstream processing steps given in group 2 |
| A. | P-3 ,Q-1 ,R-4 , S-2 |
| B. | P-2 ,Q-1 ,R-4 , S-3 |
| C. | P-4 ,Q-3 ,R-1 , S-2 |
| D. | P-4 ,Q-1 ,R-2 , S-3 |
| Answer» E. | |
| 23. |
Match the properties in Group 1 with the downstream operation in Group 2 : |
| A. | P-3, Q-4, R-2, S-1 |
| B. | P-4, Q-1, R-2, S-3 |
| C. | P-4, Q-3, R-1, S-2 |
| D. | P-3, Q-2, R-4, S-1 |
| Answer» B. P-4, Q-1, R-2, S-3 | |
| 24. |
Match the items in Group I with Group II. |
| A. | P - 4 , Q - 1 , R - 2 , S - 3 |
| B. | P - 1 , Q - 4 , R - 3 , S - 2 |
| C. | P - 2 , Q - 3 , R - 4 , S - 1 |
| D. | P - 3 , Q - 4 , R - 1 , S - 2 |
| Answer» E. | |
| 25. |
Match the entries in Group I with the methods of sterilization in Group II. |
| A. | P - 5 , Q - 3 , R - 1 , S - 4 |
| B. | P - 1 , Q - 4 , R - 5 , S - 3 |
| C. | P - 2 , Q - 1 , R - 4 , S - 3 |
| D. | P - 4 , Q - 1 , R - 3 , S - 5 |
| Answer» D. P - 4 , Q - 1 , R - 3 , S - 5 | |
| 26. |
Match each parameter in group 1 with the appropriate measuring device in group 2 |
| A. | P-3, Q-4, R-l, S-2 |
| B. | P-l, Q-3, R-2, S-4 |
| C. | P-4, Q-l, R-2, S-3 |
| D. | P-l, Q-2, R-3, S-4 |
| Answer» B. P-l, Q-3, R-2, S-4 | |