The molar extinction coefficients of Trp and Tyr at 280 nm are 5690 and 1280 M 1.cm 1, respectively. The polypeptide chain of yeast alcohol dehydrogenase (37 kDa) contains 5 Trp and 14 Tyr residues. The absorbance at 280 nm of a 0.32 mg.mL 1 solution of yeast alcohol dehydrogenase measured in a cuvette of 1 cm pathlength will be _________. (Assume that the molar extinction coefficient values for Trp and Tyr apply to these amino acids in the yeast alcohol dehydrogenase).

The molar extinction coefficients of Trp and Tyr at 280 nm are 5690 an

1.	The molar extinction coefficients of Trp and Tyr at 280 nm are 5690 and 1280 M 1.cm 1, respectively. The polypeptide chain of yeast alcohol dehydrogenase (37 kDa) contains 5 Trp and 14 Tyr residues. The absorbance at 280 nm of a 0.32 mg.mL 1 solution of yeast alcohol dehydrogenase measured in a cuvette of 1 cm pathlength will be _________. (Assume that the molar extinction coefficient values for Trp and Tyr apply to these amino acids in the yeast alcohol dehydrogenase).
A.	0.37 to 0.43
B.	0.37
C.	0.23
D.	None of the above
Answer» B. 0.37

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