A protein is to be purified using ion- exchange column chromatography. The relationship between HETP (Height Equivalent to Theoretical Plate) and the linear liquid velocity of mobile phase is given by :H = A + Bu + Cuwhere H is HETP (m) and u is linear liquid velocity of mobile phase (m.s 1). The values of A, B and C are 3 10 8 m2.s 1, 3 s and 6 10 5 m, respectively. The number of theoretical plates based on minimum HETP for a column of 66 cm length will be ___________.

A protein is to be purified using ion- exchange column chromatography.

1.	A protein is to be purified using ion- exchange column chromatography. The relationship between HETP (Height Equivalent to Theoretical Plate) and the linear liquid velocity of mobile phase is given by :H = A + Bu + Cuwhere H is HETP (m) and u is linear liquid velocity of mobile phase (m.s 1). The values of A, B and C are 3 10 8 m2.s 1, 3 s and 6 10 5 m, respectively. The number of theoretical plates based on minimum HETP for a column of 66 cm length will be ___________.
A.	2000
B.	500
C.	1000
D.	All of the above
Answer» D. All of the above

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